Cardioprotective effect of amlodipine in oxidative stress induced by experimental myocardial infarction in rats

Authors

  • Sudhira Begum Department of Pharmacology, Bangabandhu Sheikh Mujib Medical University, Shahbag, Dhaka 1000
  • Nargis Akhter Department of Pharmacology, Bangabandhu Sheikh Mujib Medical University, Shahbag, Dhaka 1000

DOI:

https://doi.org/10.3329/bjp.v2i2.570

Keywords:

Amlodipine, Aspertate aminotransferase, Cardioprotection, Lactate dehydrogenase, Malondialdehyde

Abstract

The present study investigated whether the administration of amlodipine ameliorates oxidative stress induced by experimental myocardial infarction in rats. Adrenaline was administered and myocardial damage was evaluated biochemically [significantly increased serum aspertate aminotransferase (AST), lactate dehydrogenase (LDH) and malondialdehyde (MDA) levels of myocardial tissue] and histologically (morphological changes of myocardium). Amlodipine was administered as pretreatment for 14 days in adrenaline treated rats. Statistically significant amelioration in all the biochemical parameters supported by significantly improved myocardial morphology was observed in amlodipine pretreatment. It was concluded that amlodipine afforded cardioprotection by reducing oxidative stress induced in experimental myocardial infarction of catecholamine assault.

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References

Boon NA, Fox KAA, Bloomfield P. Disease of cardiovascular system. In. Davidson,s Principles and practice of medicine. Haselett C, Chilvers ER, Hunter JAA, Boon NA, (eds). 18th ed. Edinbergh, Churchill Livingstone, 1999, p 191.

Debashis B, Aindrila C, Goutam G, Asoke GD. Oxidative stress-induced ischemic heart disease protected by antioxidant. Curr Med Chem. 2004; 11: 369-87.

Gatsura SV. Oxygen-dependent mechanisms underlying the antiischemic effect of verapamil and amlodipine. Bull Exp Biol Med. 2004; 137: 40-42.

Gupta M, Chari S. Lipid peroxidation and antioxidant enzymes in coronary and peripheral artery disease. Indian J Med Sci. 2001; 55: 79-82.

Hagan TM, Inger Soll RT, Laykkesfeld J, Liu J, Wehr CM, Vinarsky V, Bartholomew JC, Ames BN. (R)-2-Lipoic acid supplemented old rats have improved mitochondrial function, decreased oxidative damage and increased metabolic rate. FASEB J. 1999; 13: 411-18.

Isil M, Sara H, Nevbahar T, Zuhal P, Dilek O, Oya B, Aysegul U. Amlodipine and glutathione cycle in hypercholesterolaemia. Acta Cardiologica. 2004; 59: 485-92.

Karlsberg RP, Cryer PE, Roberts R. Serial plasma catecholamine response early in the course of clinical acute myocardial infarction: Relationship to infarct extent and mortality. Am Heart J. 1981; 102: 24-29.

Khan I. Effect of antioxidant vitamin E and C on adrenaline induced myocardial damage in rats. M. Phil Thesis, Dhaka, University of Dhaka. 1996.

Khatun M, Chowdhury SAR, Misbahuddin M. Infarct like myocardial lesion produced by catecholamine in rat. Bangladesh J Physiol Pharmacol. 1996; 12: 4-5.

Landmark K, Abdelnoor M, Kilhovd B, Dorum HP. Infarct size as estimated from peak creatine kinase and lactate dehydrogenase is probably reduced in patients using calcium antagonists at the onset of symptoms. Cardiovasc Drugs Ther. 1997:11: 557-65.

Lemely JM, Meneely GR. Effect of anoxia on metabolism of myocardial tissue. Am J Med. 1952; 2: 66-73.

Lovric- Bencic M, Sikiric P, Hanzevacki LS, Seiwerth S, Rogic D, Kusec V, Aralica G, Konjevoda P, Batelja L, Blagaic AB. Doxorubicine-congestive heart failure-increased big endothelin-1 plasma concentration: reversal by amlodipine, losartan and gastric pentadecapeptide BPC157 in rats and mouse. J Pharmacol Sci. 2004; 95: 19-26.

Lowary OH, Rosenbrough NJ, Farr AL, Randall RJ. Protein measurement with the folin phenol reagent. J Biol Chem. 1951; 265-275.

Lucchesi BR, Hoff PT, Tamura Y. Cardioprotective effects of amlodipine in animal models of ischemia and reperfusion. J Cardiovasc Pharmacol. 1991; 1: 34-39.

Mason RP, Mak IT, Trumbore MW, Mason PE, Milton P, Alan M. Antioxidant properties of calcium antagonists related to membrane biophysical interactions. Am J Cardiol. 1999; 84: 16-22.

Mathieu M. IFCC method for L-Aspertate aminotransferase. J Clin Chem Clin Biochem. 1986; 24: 497-510. Cited by BIOLAB in document provided with AST (GOT) IFCC Single Vial Method. Package 80125.

Mohibullah AKM, Hossain MFA, Chowdhury FI, Islam MN. Pattern of cardiovascular diseases in Sir Salimullah Medical College and Mitford Hospital, Dhaka a six-year study. Sir Salimullah Med Coll J. 1998; 6: 13-16.

Nahar N, Akhter N, Rahman MS. Protective role of carvidilol in experimental myocardial infarction. Bangladesh J Physiol Pharmacol. 2004; 20: 9-12.

Pandry NR, Kaur G, Chandra M, Sanwal GG, Misra MK. Enzymatic oxidant and antioxidants of human blood platelets in unstable angina and myocardial infarction. Int J Cardiol. 2000; 76: 33-38.

Pushpa B, Chandra M, Misra WK. Oxidative stress parameters in erythocytes of post-reperfused patients with infarction. J Enz Inhib Med Chem. 2005; 20: 377-81.

Rahman W. Role of nonselective beta-blockers in the prevention of adrenaline induced cardiac damage following myocardial infarction. M.Phil Thesis Dhaka. BSMMU. 2007.

Rona G, Chappel CL, Balazs T, Gaudry R. An infarct like myocardial lesion and other toxic manifestations produced by isoproterenol in the rat. AMA Arch Pathol. 1959; 67: 443-55.

Singal PK, Kapur N, Dhillon KS, Beamish RE, Dhalla NS. Role of free radicals in catecholamine-induced cardiomyopathy. Can J Physiol Pharmacol. 1981; 60: 1390-97.

Yogeeta SK, Gnanapragasam A, Kumar SS, Subhashini R, Sathivel A, Devaki T. Synergistic interactions of ferulic acid with ascorbic acid: Its cardioprotetive role during isoproterenol-induced myocardial infarction in rats. Mol Cell Biochem. 2006; 283: 139-46.

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How to Cite

Begum, S., and N. Akhter. “Cardioprotective Effect of Amlodipine in Oxidative Stress Induced by Experimental Myocardial Infarction in Rats”. Bangladesh Journal of Pharmacology, vol. 2, no. 2, June 2007, pp. 55-60, doi:10.3329/bjp.v2i2.570.

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Research Articles