In vitro displacement interaction of atenolol and amlodipine on binding with bovine serum albumin when co-administered

  • Md. Ashraful Alam Department of Pharmacology, Bangladesh Agricultural University, Mymensingh 2202
  • Md. Abdul Awal Department of Pharmacology, Bangladesh Agricultural University, Mymensingh 2202
  • Mahbub Mostofa Department of Pharmacology, Bangladesh Agricultural University, Mymensingh 2202
  • Md. Kamrul Islam Department of Physiology, Bangladesh Agricultural University, Mymensingh 2202
  • Nusrat Subhan Pharmacy Discipline, Khulna University, Khulna 9208
Keywords: Amlodipine, Atenolol, Equilibrium dialysis, Interaction


The binding of atenolol (selective ?1-blocker) and amlodipine (calcium channel blocker) to bovine serum albumin (BSA) was studied by equilibrium dialysis method in order to have an insight into the binding chemistry of these two to BSA. Free atenolol concentration was increased due to addition of amlodipine which reduced the binding of the compounds to BSA. However, the free fraction was increased to a level as it was expected from direct competitive displacement while the free atenolol concentration was increased according to increasing the amlodipine concentration when only the BSA was present. The result obtained when the binding site was blocked by sufficient amount of amlodipine was that the increment of free concentration of atenolol was prominent. When no amlodipine was added the free concentration of atenolol was only 28% whereas this release was 93 % to 98.01% when amlodipine was added with increasing concentration.


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How to Cite
Alam, M., M. Awal, M. Mostofa, M. Islam, and N. Subhan. “In Vitro Displacement Interaction of Atenolol and Amlodipine on Binding With Bovine Serum Albumin When Co-Administered”. Bangladesh Journal of Pharmacology, Vol. 2, no. 1, 1, pp. 20-26, doi:10.3329/bjp.v2i1.496.
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