Larvicidal activity of Cestrum nocturnum on Aedes aegypti


  • Chetan Jawale Department of Zoology, HPT Arts & RYK Science College, Nashik 422005, Maharashtra
  • Rambhau Kirdak Directorate of Higher Education, Pune, Maharashtra
  • Laxmikant Dama Department of Zoology, D. B. F. Dayanand College of Arts and Science, Solapur 413004, Maharashtra



Aedes aegypti, Cestrum nocturnum, Dengue fever, Larvicidal


Aedes aegypti is a vector parasite of the Dengue. New method to control the population of this insect is necessary. In the present work we evaluated the potential of extract from Cestrum nocturnum as larvicide. Methanol extract outstand as highly active larvicide, achieving  100% larval mortality in 24 hours when tested in the concentration of 45 µg/mL (soxhlet) and 25 µg/mL (percolation). Any extract exhibiting significant larvicide activity was further fractioned and the fraction tested according to the WHO protocol. One fraction derived from methanol extract present remarkable LC100 at 12 µg/mL. LC50 of methanol extract and active fraction were found 14 µg/mL and 6 µg/mL respectively. These fractions will be submitted to further fractions aiming to identify the molecules responsible for the larvicide activity.


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Author Biography

Laxmikant Dama, Department of Zoology, D. B. F. Dayanand College of Arts and Science, Solapur 413004, Maharashtra

Assistant Professor


Finney DJ. Probit analysis. Cambridge (England), Cambridge University Press, 1971.

Fulmali PV, Walimbe A, Mahadev PV. Spread, establishment and prevalence of dengue vector Aedes aegypti (L.) in Konkan region, Maharashtra, India. Indian J Med Res. 2008; 127: 589-601.

Ghosh A, Chandra G. Biocontrol efficacy of Cestrum diurnum L. (Solanaceae: Solanales) against the larval forms of Anopheles stephensi. Nat Prod Res. 2006; 20: 371-79.

Ghosh A, Chowdhury N, Chandra G. Laboratory evaluation of phytosteroid compound of mature leaves of Day Jasmine (Solanaceae: Solanales) against larvae of Culex quinquefasciatus (Diptera: Culicidae) and nontarget organisms. Parasitol Res. 2008; 103: 271-77.

Kumar K, Chhabra M, Katyal R, Patnaik PK, Kukreti H, Rai A, Saxena VK, Mittal V, Lal S. Investigation of an outbreak of Chikungunya in Malegaon municipal areas of Nashik District, Maharashtra (India) and its control. J Vector Borne Dis. 2008; 45: 157-63.

Mimaki Y, Wantanabe K, Sakagami H, Sashida Y. Steroidal glycosides from the leaves of Cestrum nocturnum. J Nat Prod. 2002; 65: 1863-68.

Pelah D, Abramovich Z, Markus A, Wiesman Z. The use of commercial saponin from Quillaja saponaria bark as a natural larvicidal agent against Aedes aegypti and Culex pipiens. J Ethnopharmacol. 2002; 81: 407-09.

Sukumar K, Perich MJ, Boobar LR. Botanical derivatives in mosquito control: A review. J Am Mosq Control Assoc. 1991; 7: 210-37.

World Heath Organization, Instructions for determining the susceptibility or resistance of mosquito larvae to insecticides. WHO/VBC. 1981; 81: 807-962.

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How to Cite

Jawale, C., R. Kirdak, and L. Dama. “Larvicidal Activity of Cestrum Nocturnum on Aedes Aegypti”. Bangladesh Journal of Pharmacology, vol. 5, no. 1, June 2010, pp. 39-40, doi:10.3329/bjp.v5i1.4714.



Research Articles