LC-MS analysis, antioxidant and alpha-glucosidase inhibitory activities of Centaurea papposa extracts

Authors

  • Cheribet Mawahib Valorization of Natural Resources, Bioactive Molecules and Biological Analysis Unit, Department of Chemistry, University of Mentouri Constantine, Constantine 25000, Algeria. http://orcid.org/0000-0001-6807-5090
  • Zaabat Nabila Valorization of Natural Resources, Bioactive Molecules and Biological Analysis Unit,Department of Chemistry, University of Mentouri Constantine, Constantine 25000, Algeria. http://orcid.org/0000-0002-3661-7366
  • Souilah Nabila Valorization of Natural Resources, Bioactive Molecules and Biological Analysis Unit, Department of Chemistry, University of Mentouri Constantine, Constantine 25000, Algeria. http://orcid.org/0000-0001-7762-9657
  • Bensouici Chawki Laboratory of Biochemistry, Biotechnology and Health Division, Center for Research in Biotechnology, Constantine 25000, Algeria. http://orcid.org/0000-0003-4612-4642
  • Akkal Salah Valorization of Natural Resources, Bioactive Molecules and Biological Analysis Unit, Department of Chemistry, University of Mentouri Constantine, Constantine 25000, Algeria. http://orcid.org/0000-0002-4999-5673

DOI:

https://doi.org/10.3329/bjp.v14i4.41246

Keywords:

Alpha-glucosidase inhibitor, Antioxidant, Centaurea papposa

Abstract

This work aimed to ascertain the phenolic compounds and assess the antioxidant capacity and alpha-glucosidase inhibitory activity of Centaurea papposa extracts. Phenolic compounds were appraised using LC-MS technique. Moreover, antioxidant activity was investigated using DPPH, ABTS, CUPRAC and FRAP assays. In vitro alpha-glucosidase inhibitory effect was carried out. LC-MS analysis revealed the presence of 21 compounds among which 13 were phenolic acids, 6 flavonoids, 1 phenolic aldehyde and 1 benzopyrone. The ethyl acetate extract exhibited the highest activity in ferric reducing antioxidant power (FRAP) assay (IC50: 22.9 ± 2.8 µg/mL). Nevertheless the n-butanol extract was the most active in cupric reducing antioxidant capacity assay (IC50: 3.1 ± 0.1 µg/mL). A significant alpha-glucosidase inhibitory activity was displayed by dichloromethane extract (IC50: 227.6 ± 4.4 µg/mL).

Video Clip of Methodology:

Cupric reduction antioxidant capacity: 1 min 47 sec:  Click to watch

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Published

2019-12-13

How to Cite

Mawahib, C., Z. Nabila, S. Nabila, B. Chawki, and A. Salah. “LC-MS Analysis, Antioxidant and Alpha-Glucosidase Inhibitory Activities of Centaurea Papposa Extracts”. Bangladesh Journal of Pharmacology, vol. 14, no. 4, Dec. 2019, pp. 159-65, doi:10.3329/bjp.v14i4.41246.

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Section

Research Articles