Antidepressant, neuropharmacological activity and mode of action of theaflavin-3-gallate in in vitro and in vivo models of depression

Authors

  • Ying Wang School of Psychology, North China University of Science and Technology, Tangshan, Hebei 063000, China http://orcid.org/0000-0003-4670-4786
  • Jing Chen College of Life Sciences, North China University of Science and Technology, Tangshan, Hebei 063000, China http://orcid.org/0000-0001-7721-2608
  • Jing Wang Department of Clinic, Tangshan Vocational and Technical College, Tangshan, Hebei 063000, China
  • Xin Guo School of Graduate, North China University of Science and Technology, Tangshan, Hebei 063000, China
  • Ning Wang School of Graduate, North China University of Science and Technology, Tangshan, Hebei 063000, China http://orcid.org/0000-0001-8090-1412
  • Jie Yuan Institute of Mental Health, North China University of Science and Technology, Tangshan, Hebei 063000, China. http://orcid.org/0000-0001-7103-111X

DOI:

https://doi.org/10.3329/bjp.v13i4.37773

Keywords:

Antidepressant, MTT

Abstract

Herein the antidepressant-like effect of theaflavin-3-gallate, a plant polyphenolic compound in 1-methyl-4-phenyl pyridinium-induced neuronal injury in N2a cells used as an in vitro model of depression as well as in vivo. The results from MTT (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide) and lactic dehydrogenase assay revealed that theaflavin-3-gallate exerted a neuroprotective effect in N2a cells. Treatments with mixtures of 1-methyl-4-phenyl pyridinium (20 µM) and four concentration levels of theaflavin-3-gallate increased cell viability from 23.3 to 79.6% while lactic dehydrogenase leakage value decreased from 63.2 to 23.4 U/L. Fura-2-acetoxymethyl ester assay for Ca2+ concentration measurement revealed that different doses of theaflavin-3-gallate or fluoxetine (20 µM) reduced the intracellular Ca2+ overloading in N2a cells induced by 1-methyl-4-phenyl pyridinium. Inverted phase microscopy showed that theaflavin-3-gallate induced neurite outgrowth in 1-methyl-4-phenyl pyridinium-induced neurotoxicity in N2a cells. Theaflavin-3-gallate could significantly reduce the immobility time of mice in both forced swimming test and tail suspension test.

Video Clip of Methodology:

MTT Assay: 3 min 38 sec  Click to watch

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Published

2018-11-21

How to Cite

Wang, Y., J. Chen, J. Wang, X. Guo, N. Wang, and J. Yuan. “Antidepressant, Neuropharmacological Activity and Mode of Action of Theaflavin-3-Gallate in in Vitro and in Vivo Models of Depression”. Bangladesh Journal of Pharmacology, vol. 13, no. 4, Nov. 2018, pp. 340-8, doi:10.3329/bjp.v13i4.37773.

Issue

Vol. 13 No. 4 (2018)

Section

Research Articles

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