Isolation of phytoconstituents and evaluation of biological potentials of Berberis hispanica from Algeria

  • Redouane Lemoui Unité de Recherche Valorisation des Ressources Naturelles, Molécules Bioactives et Analyses Physicochimiques et Biologiques. Université Frères Mentouri, Constantine, Route d’Ain El Bey 25000, Constantine http://orcid.org/0000-0002-7084-8758
  • Samira Benyahia Unité de Recherche Valorisation des Ressources Naturelles, Molécules Bioactives et Analyses Physicochimiques et Biologiques. Université Frères Mentouri, Constantine, Route d’Ain El Bey 25000, Constantine http://orcid.org/0000-0002-2382-9669
  • Labib Noman Laboratory of Plant Research, Department of Chemistry, Faculty of Science, Cankiri Karatekin University, Cankiri
  • Ilham Bencherchar Unité de Recherche Valorisation des Ressources Naturelles, Molécules Bioactives et Analyses Physicochimiques et Biologiques. Université Frères Mentouri, Constantine, Route d’Ain El Bey 25000, Constantine
  • Feyza Oke-Altuntas Department of Biology, Faculty of Science, Gazi University, Ankara 06500 http://orcid.org/0000-0001-6696-9090
  • Khellaf Rebbas Université de M’Sila, 28000 M’Sila http://orcid.org/0000-0002-2846-3838
  • Samir Benayache Unité de Recherche Valorisation des Ressources Naturelles, Molécules Bioactives et Analyses Physicochimiques et Biologiques. Université Frères Mentouri, Constantine, Route d’Ain El Bey 25000, Constantine http://orcid.org/0000-0003-2298-2466
  • Fadila Benayache Unité de Recherche Valorisation des Ressources Naturelles, Molécules Bioactives et Analyses Physicochimiques et Biologiques. Université Frères Mentouri, Constantine, Route d’Ain El Bey 25000, Constantine http://orcid.org/0000-0002-2282-6343
  • Ibrahim Demirtas Laboratory of Plant Research, Department of Chemistry, Faculty of Science, Cankiri Karatekin University, Cankiri http://orcid.org/0000-0001-8946-647X
Keywords: Berberis hispanica, Phytoconstituent

Abstract

The aim of this study was to isolate the phytoconstituents and evaluate the biological activities of Berberis hispanica. Three phenolic compounds (tamarixetin, caffeic acid and rutin)  were isolated from B. hispanica. The structures of the pure compounds were elucidated by spectroscopic and mass-spectrometric analyses, including 1D-, 2D-NMR, and HPLC-TOF/MS. In addition, antimicrobial, anti-oxidant and anti-proliferative effects of the extracts, some fractions and isolated compounds were evaluated. The extracts of B. hispanica were evaluated against six bacterial strains and exhibited the highest activity against Klebsiella pneumonia (15 mm at 100 mg/mL). Fraction T36 (IC50<5 μg/mL) from the n-butanol extract displayed higher radical scavenging activity than butylated hydroxytoluene. The isolated compounds were evaluated for their antiproliferative effects against human cervical adenocarcinoma (HeLa) cell line by real time cell analyzer assay and tamarixetin exhibited the remarkable effect (IC50<50 μg/mL) on HeLa cells. This study supports the documented medicinal effects of B. hispanica.

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Antiproliferative Assay: 4 min 31 sec   Click to watch

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Author Biography

Ibrahim Demirtas, Laboratory of Plant Research, Department of Chemistry, Faculty of Science, Cankiri Karatekin University, Cankiri
Bioactivity of natural products

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Published
2018-06-07
How to Cite
Lemoui, R., S. Benyahia, L. Noman, I. Bencherchar, F. Oke-Altuntas, K. Rebbas, S. Benayache, F. Benayache, and I. Demirtas. “Isolation of Phytoconstituents and Evaluation of Biological Potentials of Berberis Hispanica from Algeria”. Bangladesh Journal of Pharmacology, Vol. 13, no. 2, June 2018, pp. 179-86, doi:10.3329/bjp.v13i2.36133.
Section
Research Articles