Chemical analysis of phenolic compounds and determination of antioxidant, antimicrobial and cytotoxic activities of organic extracts of Pinus coulteri

Authors

DOI:

https://doi.org/10.3329/bjp.v13i2.35479

Keywords:

Antimicrobial, Antioxidant, Cytotoxic, Pinus coulteri

Abstract

New bioactive natural products, the phenolic composition and the biological activities of organic extracts from the needles of the Algerian Pinus coulteri were investigated. The analysis by HPLC-DAD of crude extract revealed the presence of 10 phenolic acids and nine flavonoids. In vitro anti-oxidant activities were performed using four different tests. The greatest antiradical activity was found in the ethyl acetate fraction (IC50 = 3.2 ± 0.3 µg/mL), whereas the diethyl ether fraction had the higher contents of total phenolics and flavonoids and exhibited a highest activity in reducing power and β-carotene–linoleic acid tests with EC50= 67.1 ± 0.4 μg/mL and 71.5 ± 0.2% of inhibition, respectively. Furthermore, a low to moderate antimicrobial activity according to all extracts was revealed against eight bacteria tested. The MIC value of chloroform fraction showed a strong degree of antibacterial activity (<0.09 mg/mL). The crude extract was found toxic with LC50 value of 15.2 μg/mL by brine shrimp toxicity assay. The needle extract of P. coulteri is rich in valuable biologically active compounds and could represent a new resource of anti-oxidant agents for the treatment of diseases.

Video Clip of Methodology:

Disc diffusion test: 6 min 41 sec   Click to watch

Downloads

Download data is not yet available.
Abstract
2903
Download
1968 Read
822

Downloads

Additional Files

Published

2018-04-10

How to Cite

Merah, S., D. Dahmane, S. Krimat, H. Metidji, A. Nouasri, L. Lamari, and T. Dob. “Chemical Analysis of Phenolic Compounds and Determination of Antioxidant, Antimicrobial and Cytotoxic Activities of Organic Extracts of Pinus Coulteri”. Bangladesh Journal of Pharmacology, vol. 13, no. 2, Apr. 2018, pp. 120-9, doi:10.3329/bjp.v13i2.35479.

Issue

Vol. 13 No. 2 (2018)

Section

Research Articles

License

Authors who publish with this journal agree to the following terms:

  1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).