Antioxidant, DNA-damage protection and anti-cancer properties of n-butanol extract of the endemic Perralderia coronopifolia

  • Khadidja Bekhouche Departement of Animal Biology, Faculty of Natural and Life Sciences Unversity of Mentouri 1 Constantine http://orcid.org/0000-0002-1039-6681
  • Tevfik Ozen Department of Chemistry, Faculty of Science and Arts, Ondokuz May?s University, Samsun http://orcid.org/0000-0003-0133-5630
  • Sara Boussaha Research Unit and Valuation of Natural Resources, Bioactive Molecules, Physicochemical and Biological Analyzes (VARENBIOMOL), Constantine University 1 Constantine
  • Serkan Koldas Plant Research Laboratory, Department of Chemistry, University of Cankiri Karatekin, Cankiri http://orcid.org/0000-0002-7917-4008
  • Semiha Yenigun Department of Chemistry, Faculty of Science and Arts, Ondokuz May?s University, Samsun http://orcid.org/0000-0002-1979-5427
  • Somia Lassed Departement of Animal Biology, Faculty of Natural and Life Sciences Unversity of Mentouri 1 Constantine http://orcid.org/0000-0003-1684-880X
  • Ibrahim Demirtas Plant Research Laboratory, Department of Chemistry, University of Cankiri Karatekin, Cankiri http://orcid.org/0000-0001-8946-647X
  • Fadila Benayache Research Unit and Valuation of Natural Resources, Bioactive Molecules, Physicochemical and Biological Analyzes (VARENBIOMOL), Constantine University 1 Constantine http://orcid.org/0000-0002-2282-6343
  • Samir Benayache Research Unit and Valuation of Natural Resources, Bioactive Molecules, Physicochemical and Biological Analyzes (VARENBIOMOL), Constantine University 1 Constantine http://orcid.org/0000-0003-2298-2466
  • Djamila Zama Departement of Animal Biology, Faculty of Natural and Life Sciences Unversity of Mentouri 1 Constantine
Keywords: Anti-cancer, Antioxidant, DNA-damage, Perralderia coronopifolia

Abstract

This study was designed to evaluate in vitro the total phenolic, flavonoid content, antioxidant activity, oxidative DNA-damage protection and anti-cancer activity of n-butanol extract from Perralderia coronopifolia, endemic plant. DNA protection capacity was performed using 46966 plasmid DNA against UV-photolysis of H2O2-induced oxidative damage. The antiproliferative effects of extract were performed on HeLa and C6 cells. Experimental results showed that extract had convenient number of phenolic and flavonoids. Furthermore, it provided strong anti-oxidant, reducing power, hydrogen peroxide and higher DPPH· scavenging ability with IC50= 7.02 ± 0.02 µg/mL. As it shown an oxidative plasmid DNA-damage inhibition against UV-photolysis of H2O2, an anti-cancer activity athigher concentrations in cells. The data obtained that P. coronopifolia extract could be useful in human protection against infection and degenerative illness.

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Published
2018-03-15
How to Cite
Bekhouche, K., T. Ozen, S. Boussaha, S. Koldas, S. Yenigun, S. Lassed, I. Demirtas, F. Benayache, S. Benayache, and D. Zama. “Antioxidant, DNA-Damage Protection and Anti-Cancer Properties of N-Butanol Extract of the Endemic Perralderia Coronopifolia”. Bangladesh Journal of Pharmacology, Vol. 13, no. 1, Mar. 2018, pp. 82-89, doi:10.3329/bjp.v13i1.34255.
Section
Research Articles