Separation and evaluation of natural antileishmanial potential against Leishmania major and infuntum isolated from the Tunisia strains

Authors

  • Salwa Bouabdallah Environmental Biomonitoring Laboratory LBE (LR01/ES14), Faculty of Sciences of Bizerta
  • Dhafer Laouini Laboratory of Transmission, Control and Immunobiology of Infection (11 LR IPT 02), Pasteur Institute
  • Nabiha Bouzouita High School of Food Industry Tunis (ESIAT), Tunisia; Tunis Faculty of Sciences, University of Tunis El-Manar, 2092 Tunis
  • Safia El-Bok Laboratory of Biodiversity, Biotechnology and Climate Changes, Department of Biology, Tunis Faculty of Sciences, University of Tunis El-Manar, El-Manar, 2092 Tunis
  • Rabiaa M. Sghaier Laboratory of Transmission, Control and Immunobiology of Infection (11 LR IPT 02), Pasteur Institute
  • Sawssen Selmi Laboratory of Bioactive Substances, Biotechnology Centre in Borj-CedriaTechnopol, BP. 901, 2050 Hammam-lif
  • Mossadok Ben Attia Environmental Biomonitoring Laboratory LBE (LR01/ES14), Faculty of Sciences of Bizerta

DOI:

https://doi.org/10.3329/bjp.v13i1.33908

Keywords:

Antileishmanial, Leishmania infuntum, Leishmania major

Abstract

Besides its nutritional value as a dietary supplement, Tribulus terrestris is used as a remedy for fertility disorder in Ayurveda and Chinese medicine as well as by modern herbalists. The aim of this study was to explore the biological potential (antileishmanial effect) of an extract rich in saponins from Tunisian tribulus. The chloroform extract of the various parts of T. terrestris was subjected to partial purification by solvent partitioning with ethanol and n-butanol. All prepared extracts were tested for their anti-leishmanial activity. The result showed that n-butanolic extract (saponin fraction, when isolated from leaves part) exhibited the best antileishmanial effect against both pathogenic parasites Leshmania L. major (GlC94) and L. infuntum (LV50) evaluated in vitro assessment through MTT assay. n-Butanolic extract had been detected, quantified and purified using the RP-HPLC finger print (Hypersil ODS coupled to UV-vis). High peak area (5116.82 at 3.03 min) was detected at 205 nm.

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Published

2018-03-10

How to Cite

Bouabdallah, S., D. Laouini, N. Bouzouita, S. El-Bok, R. M. Sghaier, S. Selmi, and M. B. Attia. “Separation and Evaluation of Natural Antileishmanial Potential Against Leishmania Major and Infuntum Isolated from the Tunisia Strains”. Bangladesh Journal of Pharmacology, vol. 13, no. 1, Mar. 2018, pp. 74-81, Mar 10, 2018, doi:10.3329/bjp.v13i1.33908.

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Section

Research Articles