HPLC analysis, anti-oxidant activity of Genista ferox and its anti-proliferative effect in HeLa cell line

  • Ilhem Bencherchar Department of Chemistry, Faculty of Science, Cankiri Karatekin University, Cankiri
  • Ibrahim Demirtas Department of Chemistry, Faculty of Science, Cankiri Karatekin University, Cankiri http://orcid.org/0000-0001-8946-647X
  • Muhammed Altun Department of Chemistry, Faculty of Science, Cankiri Karatekin University, Cankiri http://orcid.org/0000-0002-0387-1819
  • Fatih Gul Department of Chemistry, Faculty of Science, Cankiri Karatekin University, Cankiri http://orcid.org/0000-0002-4297-786X
  • Djamel Sarri Valorization of Natural Resources, Bioactive Moleculesand Biological Analysis Unit, Department of Chemistry, University of Mentouri Constantine 1, Constantine 25000
  • Fadila Benayache Valorization of Natural Resources, Bioactive Moleculesand Biological Analysis Unit, Department of Chemistry, University of Mentouri Constantine 1, Constantine 25000 http://orcid.org/0000-0002-2282-6343
  • Samir Benayache Valorization of Natural Resources, Bioactive Moleculesand Biological Analysis Unit, Department of Chemistry, University of Mentouri Constantine 1, Constantine 25000 http://orcid.org/0000-0003-2298-2466
  • Ratiba Mekkiou Valorization of Natural Resources, Bioactive Moleculesand Biological Analysis Unit, Department of Chemistry, University of Mentouri Constantine 1, Constantine 25000
Keywords: Antiproliferative, Folin–Ciocalteu, Genista ferox, HPLC-TOF/MS

Abstract

The prevention and treatment of the cancer using plants have attracted increasing interest. The present study was aimed to determine the phenolic compounds of Genista ferox using HPLC-TOF/MS and the anti-oxidant acti-vity associated with anti-cancer activity against human cervical adenocarcinoma (HeLa) cell line. Total anti-oxidant capacities of different extracts of G. ferox were assessed by DPPH assay, and their total phenolic and flavonoids contents measured by FolinCiocalteu and aluminum trichloride assays. The amounts of total phenolic (105.2 ± 0.6 308.5 ± 5.7 mg/g) of extract measured as gallic acid equivalent and flavonoids (8.1 ± 0.1 124.0 ± 0.7 mg/g) of extract measured as quercetin equivalent varied from chloroform to n-butanol extract of the two parts of the plant (leaf and stem). The ethyl acetate extract of G. ferox exhibited the most powerful effect on the DPPH scavenging activity with 94% from the leaf and 93% from the stem, while the chloroform extract from the leaf exhibited the most effective anti-proliferative activity against HeLa cell lines.

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Published
2017-07-20
How to Cite
Bencherchar, I., I. Demirtas, M. Altun, F. Gul, D. Sarri, F. Benayache, S. Benayache, and R. Mekkiou. “HPLC Analysis, Anti-Oxidant Activity of Genista Ferox and Its Anti-Proliferative Effect in HeLa Cell Line”. Bangladesh Journal of Pharmacology, Vol. 12, no. 3, July 2017, pp. 260-7, doi:10.3329/bjp.v12i3.32310.
Section
Research Articles