HPLC analysis, anti-oxidant activity of Genista ferox and its anti-proliferative effect in HeLa cell line
The prevention and treatment of the cancer using plants have attracted increasing interest. The present study was aimed to determine the phenolic compounds of Genista ferox using HPLC-TOF/MS and the anti-oxidant acti-vity associated with anti-cancer activity against human cervical adenocarcinoma (HeLa) cell line. Total anti-oxidant capacities of different extracts of G. ferox were assessed by DPPH assay, and their total phenolic and flavonoids contents measured by FolinCiocalteu and aluminum trichloride assays. The amounts of total phenolic (105.2 ± 0.6 308.5 ± 5.7 mg/g) of extract measured as gallic acid equivalent and flavonoids (8.1 ± 0.1 124.0 ± 0.7 mg/g) of extract measured as quercetin equivalent varied from chloroform to n-butanol extract of the two parts of the plant (leaf and stem). The ethyl acetate extract of G. ferox exhibited the most powerful effect on the DPPH scavenging activity with 94% from the leaf and 93% from the stem, while the chloroform extract from the leaf exhibited the most effective anti-proliferative activity against HeLa cell lines.
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Abay G, Altun M, Koldas S, Tufekci AR, Demirtas I. Determination of anti-proliferative activities of volatile contents and HPLC profiles of dicranumscoparium (Dicranaceae, Bryophyta). Comb Chem High T Scr. 2015; 18: 453-63.
Belle R, Barrachina MD, Martinez-Cuesta MA, Esplugues J. Pharmacological screening of the methanol and dichloromethanol extracts of Genista patens. Phytotherapy Res. 1995; 9: 495-99.
Ciulel I. Methodology for analysis of vegetable drugs. Romania, 1982.
Demirtas I, Gecibesler IH, Yaglioglu AS. Anti-proliferative activities of isolated flavone glycosides and fatty acids from stachysbyzantina. Phytochem Lett. 2013; 6: 209-14.
Djeridane A, Yousfi M, Nadjemi B, Boutassouna D, Stocker P, Vidal N. Anti-oxidant activity of some algerian medicinal plants extracts containing phenolic compounds. Food Chem. 2006; 97: 654-60.
Dzoyem JP, McGaw, LJ, Eloff1 JN. In vitro antibacterial, anti-oxidant and cytotoxic activity of acetone leaf extracts of nine under-investigated Fabaceae tree species leads to potentially useful extracts in animal health and productivity. BMC Complement Altern Med. 2014.
Erbil N, Duzguner V, Durmuskahya C, Alan Y. Antimicrobial and anti-oxidant effects of some Turkish fodder plants belongs to Fabaceae family (viciavillosa, trifoliumo-chroleucum and onobrychisaltissima). Orient J Chem. 2015; 31: 53-58.
Fukumoto LR, Mazza G. Assessing anti-oxidant and pro-oxidant activities of phenolic compounds. J Agric Food Chem. 2000; 48; 3597-604.
Herrera MD, Marhuenda E, Gibson A. Effects of genistein, an isoflavone isolated from genista trident ata, on isolated guinea-pig ileum and guinea-pig heal myenteric plexus. Planta Med. 1992.
Krishnaiah D, Sarbatly R, Nithyanandam R. A review of the anti-oxidant potential of medicinal plant species. Food and Bioproducts Processing, 2011; 21733.
LingaRao M, Savithramma N. Phytochemical studies of svensoniahyderobadensis (Walp.) Mold: A rare medicinal plant. Pharm Lett. 2011; 3: 51-55.
Obasi Nnamdi L, Egbuonu A, Ukoha P, Ejikeme P. Comparative phytochemical and antimicrobial screening of some solvent extracts of samaneasaman (Fabaceae or mimosaceae) pods. Afr J Pure Appl Chem. 2010; 4: 206-12.
Ordonez AAL, Gomez JD, Vattuone MA, Isla MI. Anti-oxidant activities of sechiumedule (Jacq.) Swartz extracts. Food Chem. 2006; 97: 452-58.
Okten S, Erenler R, Köprülü TK, Tekin ?. In vitro anti-proliferative/cytotoxic activity of 2,3?-biindole against various cancer cell lines. Turk J Biol. 2015; 39: 15-22.
Quezel PS, Santa S. New flora of Algeria and the southern desert regions. Vol 2. Paris, Tela Botanical Association, 1963 (In French).
Re RM. Flore De Lafraque Du Nord.1987; 16.
Saeed N, Khan MR, Shabbir M. Anti-oxidant activity, total phenolic and total flavonoid contents of whole plant extracts Torilisleptophylla L. BMC Complement Altern Med. 2012; 12: 221.
Singleton V, Rossi JA. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am J Enol Viticult. 1965; 16: 144-58.
Takao T, Kitatani F, Watanabe N, Yagi A, Sakata K. A simple screening method for anti-oxidants and isolation of several anti-oxidants produced by marine-bacteria from fish and shellfish. Biosci Biotech Bioch. 1994; 58: 1780-83.
Villano De, Fern´andez-Pach´on MS, Troncoso AM, Garc´?a-Parrilla MC. Comparison of anti-oxidant activity of wine phenolic compounds and metabolites in vitro. Anal Chim Acta. 2005; 538: 391-98.
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