Evaluation of methanolic extract of Phragmites karka on carbon tetrachloride-induced liver fibrosis in rat
Keywords:Anti-fibrotic, Hepatic fibrosis, PCR, Phragmites karka, ?-Smooth muscle actin
Phragmites karka has been reported for its anti-inflammatory and analgesic activities. Here, extracts of leaf and rhizome of the plant were individually investigated in CCl4-induced hepatofibrosis in male Wistar rats by administering CCl4 intraperitoneally biweekly for 6 weeks. Afterwards the animals were investigated for liver fibrosis at biochemical, molecular and histological levels, and it showed a profound increase (p<0.001) in elevation of serum levels of transaminases, ?-glutamyl transpeptidase, mRNA expression of ? smooth muscle actin, collagen and transforming growth factor beta (TGF?), and extracellular matrix deposition and perilobular necrosis. Both extracts markedly (p<0.001) decreased the elevated levels of these markers. Histopathological investigations also substantiated the above results by exhibiting a decreased in extracellular matrix deposition in post-treatment animals. In conclusion, both extracts had substantially modified the biochemical and molecular markers of liver fibrosis, in addition to histological improvement in architecture of liver.
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