Evaluation of methanolic extract of Phragmites karka on carbon tetrachloride-induced liver fibrosis in rat

Keywords: Anti-fibrotic, Hepatic fibrosis, PCR, Phragmites karka, ?-Smooth muscle actin

Abstract

Phragmites karka has been reported for its anti-inflammatory and analgesic activities. Here, extracts of leaf and rhizome of the plant were individually investigated in CCl4-induced hepatofibrosis in male Wistar rats by administering CCl4 intraperitoneally biweekly for 6 weeks.  Afterwards the animals were investigated for liver fibrosis at biochemical, molecular and histological levels, and it showed a profound increase (p<0.001) in elevation of serum levels of transaminases, ?-glutamyl transpeptidase, mRNA expression of ? smooth muscle actin, collagen and transforming growth factor beta (TGF?), and extracellular matrix deposition and perilobular necrosis. Both extracts markedly (p<0.001) decreased the elevated levels of these markers. Histopathological investigations also substantiated the above results by exhibiting a decreased in extracellular matrix deposition in post-treatment animals. In conclusion, both extracts had substantially modified the biochemical and molecular markers of liver fibrosis, in addition to histological improvement in architecture of liver.

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Author Biographies

Atta ur Rehman, Department of Pharmacy, Forman Christian College, Lahore 54600

Assistant professor

 

Manal Liaqat, Faculty of Pharmacy, The University of Lahore, Lahore

MPhil Research Student

Rabia Asghar, Faculty of Pharmacy, The University of Lahore, Lahore

Lecturer

Nawazish-i-Husain Syed, Department of Pharmacology, Punjab University College of Pharmacy, University of the Punjab, Lahore

Associate Professor

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Published
2017-07-30
How to Cite
Rehman, A., M. Liaqat, R. Asghar, and N.- i-H. Syed. “Evaluation of Methanolic Extract of Phragmites Karka on Carbon Tetrachloride-Induced Liver Fibrosis in Rat”. Bangladesh Journal of Pharmacology, Vol. 12, no. 3, July 2017, pp. 276-81, doi:10.3329/bjp.v12i3.32127.
Section
Research Articles