Anti-inflammatory effects of Anredera cordifolia and Piper crocatum extracts on lipopolysaccharide-stimulated macrophage cell line




Anredera cordifolia, Anti-inflammatory, Inflammatory, Macrophage, Mediator, Piper crocatum


In this study, the anti-inflammatory potential of Anredera cordifolia and Piper crocatum extracts on lipopolysaccharide-induced murine macrophage cell line (RAW 264.7) was observed. Cell viability assay was performed with MTS assay. Parameters measured to determine the anti-inflammatory activity were interleukin-1? (IL-1?), tumor necrosis factor (TNF)-?, nitric oxide (NO) and IL-6. Both A. cordifolia and P. crocatum at concentration of 50 µg/mL in cell line resulted significant decrease in TNF-? level (250.3 and 242.5 pg/mL respectively). A. cordifolia showed significant decrease in IL-1? level at 50 µg/mL and IL-6 level at 10 µg/mL, whilst P. crocatum  showed significant decrease IL-1? level in three concentrations with lowest level at 50 µg/mL. A. cordifolia showed lowest decrease in NO level at 50 µg/mL but not comparable with normal cells, whilst P. crocatum showed significant decrease in NO level at 50 µg/mL. This research revealed that A. cardi folia and P. crocatum possess the anti-inflammatory potential indicated by the inhibitory activity of the inflammatory mediators including, TNF-?, IL-1?, IL-6, and NO.

Video Clip of Methodology:

Viability Assay: 4 min 8 sec   Full Screen   Alternate


Download data is not yet available.
1831 Read

Additional Files



How to Cite

Laksmitawati, D. R., A. Widyastuti, N. Karami, E. Afifah, D. D. Rihibiha, H. Nufus, and W. Widowati. “Anti-Inflammatory Effects of Anredera Cordifolia and Piper Crocatum Extracts on Lipopolysaccharide-Stimulated Macrophage Cell Line”. Bangladesh Journal of Pharmacology, vol. 12, no. 1, Mar. 2017, pp. 35-40, doi:10.3329/bjp.v12i1.28714.



Research Articles