Ferric reducing anti-oxidant power assay in plant extract

  • Maruthamuthu Vijayalakshmi Department of Pharmaceutical Technology, Bharathidasan Institute of Technology campus, Anna University, Tiruchirappalli 620024
  • Kandasamy Ruckmani Department of Pharmaceutical Technology, Bharathidasan Institute of Technology campus, Anna University, Tiruchirappalli 620024
Keywords: Ferric reducing anti-oxidant power assay, FRAP, Plant extract

Abstract

The ferric reducing anti-oxidant power (FRAP) assay involved the following steps:  a) preparation of samples, b) reactions and c) finally measuring absorbance of sample and standard at 700 nm using spectrophotometer. The samples were methanolic extract, different fractions (n-hexane, chloroform) and standard ascorbic acid. The solutions prepared were buffer solution (pH 6.6), 1% potassium ferricyanide solution, 10% trichloroacetic acid and 0.1% ferric chloride. 

Video Clip of Methodology:

Ferric reducing anti-oxidant power assay: 21 min 18 sec   Full Screen   Alternate

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References

Oyaizu M. Studies on product of browning reaction prepared from glucose amine. Jpn J Nutr. 1986; 44: 307-15.

Do QD, Angkawijaya AE, Tran-Nguyen PL, Huynh LH, Soetaredjo FE, Ismadji S, Ju YH. Effect of extraction solvent on total phenol content, total flavonoid content, and antioxidant activity of Limnophila aromatica. J Food Drug Anal. 2014; 22: 296-302.

Published
2016-06-01
How to Cite
Vijayalakshmi, M., and K. Ruckmani. “Ferric Reducing Anti-Oxidant Power Assay in Plant Extract”. Bangladesh Journal of Pharmacology, Vol. 11, no. 3, June 2016, pp. 570-2, doi:10.3329/bjp.v11i3.27663.
Section
Visual Experiment