5-Methoxyhydnocarpin shows selective anti-cancer effects and induces apoptosis in THP-1 human leukemia cancer cells via mitochondrial disruption, suppression of cell migration and invasion and cell cycle arrest

Authors

  • Xiao-Yan Xu Department of Hematology, Luoyang Central Hospital Affiliated to Zhengzhou University, Luoyang, Henan 471000
  • Hui-Rui Wang Department of Hematology, Luoyang Central Hospital Affiliated to Zhengzhou University, Luoyang, Henan 471000
  • Shu-Li Guo Department of Hematology, Luoyang Central Hospital Affiliated to Zhengzhou University, Luoyang, Henan 471000
  • Bo Li Department of Hematology, Luoyang Central Hospital Affiliated to Zhengzhou University, Luoyang, Henan 471000
  • Tao Lv Department of Hematology, Luoyang Central Hospital Affiliated to Zhengzhou University, Luoyang, Henan 471000

DOI:

https://doi.org/10.3329/bjp.v11i3.26767

Keywords:

Anti-cancer, Apoptosis, Cell cycle arrest, Cell invasion, Cell migration, 5-Methoxyhydnocarpin, Mitochondria, THP-1 human leukemia cancer cell

Abstract

The primary objective of the current investigation was to study the anti-tumor effects of 5-methoxyhydnocarpin in THP-1 human leukemia cells along with evaluating its effects on apoptotic induction, cancer cell migration and cell cycle phase distribution. MTT cell viability assay revealed the cell cytotoxic effects while as clonogenic assay revealed the effects of the compound on colony formation tendency. Phase contrast microscopy revealed the effects on cell morphology while as its effects on cell cycle and apoptosis induction were evaluated by flow cytometer. Results showed that 5-methoxyhydnocarpin induced both anchorage-dependent and anchorage-independent cytotoxic effects in THP-1 cells. 5-Methoxyhydnocarpin-treated cells exhibited significant changes in cell morphology. Flow cytometry experiment indicated that 5-methoxyhydnocarpin led to sub-G1 cell cycle arrest and also led to early and late apoptosis.

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Published

2016-06-24

How to Cite

Xu, X.-Y., H.-R. Wang, S.-L. Guo, B. Li, and T. Lv. “5-Methoxyhydnocarpin Shows Selective Anti-Cancer Effects and Induces Apoptosis in THP-1 Human Leukemia Cancer Cells via Mitochondrial Disruption, Suppression of Cell Migration and Invasion and Cell Cycle Arrest”. Bangladesh Journal of Pharmacology, vol. 11, no. 3, June 2016, pp. 652-60, doi:10.3329/bjp.v11i3.26767.

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Section

Research Articles