Zerumbone inhibits prostate cancer cell viability and induces cell death by non-apoptotic pathway

Authors

  • Xian-De Cao Department of Urology, The Affiliated Hospital of Qingdao University, Qingdao 266101; Department of Urology, The Affiliated Hospital of Jining Medical University, Jining 272029
  • Hui-Min Zheng Department of Urology, The Affiliated Hospital of Jining Medical University, Jining 272029

DOI:

https://doi.org/10.3329/bjp.v11i4.26728

Keywords:

Apoptosis, Prostate cancer cell, Zerumbone

Abstract

The aim of the present study was to investigate the role of zerumbone on the proliferation, cell cycle arrest and cell death in DU-145 prostate cancer cell lines. The MTT assay revealed that zerumbone (20 µM) reduced proliferation of DU-145 cells to 39.0% at 48 hours. It also increased the proportion of propidium iodide stained cells to 53.4% compared 1.0% in control. However, the population of annexin V-stained cells remained uneffected indicating induction of non-apoptotic cell death by zerumbone. Treatment of DU-145 cells with zerumbone (20 µM) caused 8-fold enhancement in the level of reactive oxygen species (ROS). On the other hand, exposure of the zerumbone treated DU-145 cells to glutathione inhibited the generation of ROS. Fow cytometry using propidium iodide staining revealed that zerumbone treat-ment increased proportion of cells in G1 phase to 71.3% on compared to 34.7% in the control. The results from Western blot analysis revealed a significant increase in the expression of cyclin D1 protein in DU-145 cells on treatment with 20 µM concentration of zerumbone. Thus, zerumbone treatment inhibits prostate cancer cell viability and can be used for its treatment.

Video Clip of Methodology:

Western blot assay: 3 min 37 sec  Full Screen   Alternative

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Published

2016-10-01

How to Cite

Cao, X.-D., and H.-M. Zheng. “Zerumbone Inhibits Prostate Cancer Cell Viability and Induces Cell Death by Non-Apoptotic Pathway”. Bangladesh Journal of Pharmacology, vol. 11, no. 4, Oct. 2016, pp. 771-5, doi:10.3329/bjp.v11i4.26728.

Issue

Section

Research Articles