Bryostatin I inhibits growth of breast cancer cells through the inhibition of synuclein-A expression

Authors

  • Jun-Xia Sun Department of Radiotherapy, The Second People?s Hospital of Huaian, Jiangsu 223002
  • Yan Yan Department of Radiotherapy, The Second People?s Hospital of Huaian, Jiangsu 223002
  • Jian-Hong Xia Department of Radiotherapy, The Second People?s Hospital of Huaian, Jiangsu 223002
  • Li-Qing Zhou Department of Radiotherapy, The Second People?s Hospital of Huaian, Jiangsu 223002 http://orcid.org/0000-0003-1682-5237

DOI:

https://doi.org/10.3329/bjp.v11i3.26620

Keywords:

Breast cancer cell, Bryostatin I, Synuclein-A

Abstract

The present study was aimed to investigate the effect of bryostatin I on the expression of synuclein-A in breast cancer cells. Western blot analysis showed a significant (p<0.005) reduction in the expression of synuclein-A from a concentration of 20 µM in H3922 cells. The inhibitory effect of bryostatin I on synuclein-A expression was further confirmed by the treatment of H3922 cells with known synuclein-A inhibitor, cytokine oncostatin M. Bryostatin I treatment of H3922 cells also significantly increased their sensitivity to the taxol. Incubation of the cells with 25 µM concentration of bryostatin I followed by treatment with 0.5 ?M concentration of taxol induced apoptosis in 89% cells compared to 9% cells in the taxol alone treated cultures. Treatment of the H3922 cells with bryostatin I at 25 µM concentration led to a significant increase in the activation of histone H1 protein. The results from MTT assay showed a significant decrease in the cell viability from 10 µM concentration of bryostatin I. Thus, bryostatin I inhibits the growth of breast cancer cells through inhibition of synuclein-A expression and can be used for breast cancer treatment.

Video Clip of Methodology:

Cell viability assay: 5 min 14 sec   Click to watch 

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Published

2016-06-13

How to Cite

Sun, J.-X., Y. Yan, J.-H. Xia, and L.-Q. Zhou. “Bryostatin I Inhibits Growth of Breast Cancer Cells through the Inhibition of Synuclein-A Expression”. Bangladesh Journal of Pharmacology, vol. 11, no. 3, June 2016, pp. 615-20, doi:10.3329/bjp.v11i3.26620.

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Section

Research Articles