18alpha-Glycyrrhetinic acid inhibits the viability of HR5-CL11 cervical carcinoma cells through induction of apoptosis and DNA damage

Authors

DOI:

https://doi.org/10.3329/bjp.v11i3.26592

Keywords:

18?-Glycyrrhetinic acid, Apoptosis, DNA damage, HR5-CL11 cervical carcinoma cell

Abstract

The present study was aimed to investigate the effect of 18α-glycyrrhetinic acid on induction of apoptosis and DNA-damage in HR5-CL11 cervical carcinoma cells. The results revealed 73.6% reduction in HR5-CL11 cell viability on treatment with 5 µM concentration of 18α-glycyrrhetinic acid for 48 hours. The DNA of 18α-glycyrrhetinic acid-treated cells showed a ladder-like pattern. Fragmentation of DNA in the 18α-glycyrrhetinic acid-treated cells was markedly higher compared to the control cells. Examination of the DNA damage in HR5-CL11 cells after treatment with 18α-glycyrrhetinic acid showed breakage in DNA strands and formation of comet-like structures. The frequency of comet formation in 18α-glycyrrhetinic acid treated cells was found to be 7.8 after 48 hours. The population of cells with more than four γH2AX foci was increased to 38.6% on treatment with 5 µM concentration of 18α-glycyrrhetinic acid. Thus, 18α-glycyrrhetinic acid inhibits the viability of HR5-CL11 cervical cancer cells through induction of apoptosis by DNA damage and can be used for the treatment of cervical cancer.

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MTT assay: 1 min 13 sec   Click to watch

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Published

2016-08-26

How to Cite

Zhuang, H.-M., X. Hu, S.-R. Hong, P.-P. Zhou, Y. Zhang, and L. Li. “18alpha-Glycyrrhetinic Acid Inhibits the Viability of HR5-CL11 Cervical Carcinoma Cells through Induction of Apoptosis and DNA Damage”. Bangladesh Journal of Pharmacology, vol. 11, no. 3, Aug. 2016, pp. 750-5, doi:10.3329/bjp.v11i3.26592.

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Research Articles