Modulation of adipogenesis-related gene expression by ethanol extracts of Detam 1 soybean and Jati belanda leaf in 3T3-L1 cells

Authors

  • Meilinah Hidayat Faculty of Medicine, Maranatha Christian University, Bandung
  • Sijani Prahastuti Faculty of Medicine, Maranatha Christian University, Bandung
  • Nurul Fauziah Biomolecular and Biomedical Research Center, Aretha Medika Utama, Bandung
  • Maesaroh Maesaroh Biomolecular and Biomedical Research Center, Aretha Medika Utama, Bandung
  • Balqis Balqis Biomolecular and Biomedical Research Center, Aretha Medika Utama, Bandung http://orcid.org/0000-0003-0320-5303
  • Wahyu Widowati Faculty of Medicine, Maranatha Christian University, Bandung

DOI:

https://doi.org/10.3329/bjp.v11i3.26471

Keywords:

3T3-L1 cell, Adipogenesis-related gene expression, Detam 1 soybean, Jati belanda

Abstract

In this study, we evaluated the effects of ethanol extracts of Detam 1 soybean, Jati belanda leaf, and the combination toward expression of peroxisome proliferator-activated receptor gamma (PPARγ), CCAAT/enhancer-binding protein alpha (C/EBPα), and stearoyl-CoA desaturase 1 (SCD1) genes in 3T3-L1 cells as anti-adipogenesis and anti-obesity. The differentiation of 3T3-L1 cells into adipocyte was conducted using induction medium consist of Dulbecco's Modified Eagle's Medium, 3-isobutyl-1-methylxanthine, insulin, dexamethasone, and fetal bovine serum. The expression of PPARγ, C/EBPα, and SCD1 gene was measured using real-time quantitative polymerase chain reaction (qPCR). Ethanol extract of Jati belanda at a concentration of 50 μg/mL was most effective to reduce PPARγ, C/EBPα, and SCD1 gene expression in 3T3-L1 cells. Ethanol extract of Detam 1 soybean failed to reduce PPARγ gene expression, whilst in the concentration of 50 μg/mL it was able to significantly reduce the C/EBPα and SCD1 gene expression. Both ethanol extracts of Detam 1 soybean and Jati belanda have potential as anti-adipogenesis and anti-obesity by suppressing adipogenesis-related gene expression, particularly C/EBPα and SCD1.

Video Clip of Methodology:

Maintaining 3T3-L1 cell culture: 5 min 15 sec   Click to watch

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Published

2016-07-21

How to Cite

Hidayat, M., S. Prahastuti, N. Fauziah, M. Maesaroh, B. Balqis, and W. Widowati. “Modulation of Adipogenesis-Related Gene Expression by Ethanol Extracts of Detam 1 Soybean and Jati Belanda Leaf in 3T3-L1 Cells”. Bangladesh Journal of Pharmacology, vol. 11, no. 3, July 2016, pp. 697-02, doi:10.3329/bjp.v11i3.26471.

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Section

Research Articles