Poly-phenolic fraction of Chrysophyllum cainito extract induces cell death in osteosarcoma cells

Authors

  • Liu-Bing Li Department of Orthopedics, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004, China
  • Subin Lin Department of Orthopedics, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004, China
  • Jun Yan Department of Orthopedics, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004, China
  • Qian-Liang Wang Department of Orthopedics, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004, China
  • Zhi-Ying Fan Department of Orthopedics, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004, China
  • Qi-Rong Dong Department of Orthopedics, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004, China
  • Jian-Zhong Qin Department of Orthopedics, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004, China http://orcid.org/0000-0002-5674-8400
  • Zong-Gang Xie Department of Orthopedics, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004, China

DOI:

https://doi.org/10.3329/bjp.v10i4.24576

Keywords:

Cell death, Chrysophyllum cainito, Osteosarcoma cell, Poly-phenolic

Abstract

Osteosarcoma shows resistance to chemotherapy and many side effects. The ethyl acetate fraction of ethanolic extract of Chrysophyllum cainito was taken in the present work. The extract was characterized for its phenolic content, antioxidant capacity (ferric reducing antioxidant power and 2,2-diphenyl-1-picrylhydrazyl assays), ability to form reactive oxygen species and annexin V assay. The extract contained phenolic content of 30.1 ± 0.2 mg GAE/g extract. Ferric reducing antioxidant power assay and DPPH assay showed 213.2 ± 12.4 mM Fe2+ equivalent per gram extract and 85.6 ± 4.9 TE/g respectively. For production of reactive oxygen species in the osteosarcoma cells, extract at 50 µg GAE/mLconcentration was statistically equivalent to 100 µM H2O2. EC50 of extract was calculated to be 133 µg GAE/mL in cell viability studies.

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Published

2015-11-24

How to Cite

Li, L.-B., S. Lin, J. Yan, Q.-L. Wang, Z.-Y. Fan, Q.-R. Dong, J.-Z. Qin, and Z.-G. Xie. “Poly-Phenolic Fraction of Chrysophyllum Cainito Extract Induces Cell Death in Osteosarcoma Cells”. Bangladesh Journal of Pharmacology, vol. 10, no. 4, Nov. 2015, pp. 972-9, doi:10.3329/bjp.v10i4.24576.

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Section

Research Articles