Inhibitory effect of schizophyllan on rat glioma cells

Authors

  • Bin Zhou Department of Neurosurgery, the Second Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan 250001
  • Qiang Fu Department of Neurosurgery, Liaocheng People's Hospital, Liaocheng 252002
  • Sha-Sha Song Department of Pathology, Ji'nan Central Hospital Affiliated to Shandong University, Jinan 250100
  • Hong-Li Zheng Department of Neurosurgery, people's Hospital of Haiyang City, Haiyang 265100
  • Yu-Zhen Wei Department of Neurosurgery, Jining No.1 People?s Hospital, Jining 272011 http://orcid.org/0000-0003-1032-9451

DOI:

https://doi.org/10.3329/bjp.v10i4.23834

Keywords:

Glioma cell, Rat, Schizophyllan

Abstract

The aim of this study was to examine the anticancer effects of schizophyllan (a -D-glucan) against the growth of rat CNS-1 glioma cells and preliminarily assess its effect on inducing apoptosis and blocking cell cycle. In order to evaluate its inhibitory effect, firstly MTT assay was conducted followed by annexin V/propidium iodide double staining or propidium iodide single staining, apoptosis and cell cycle using flow cytometry. All the experiments were carried in a dose- and time-dependent manner. Experimental results showed that treatment of 40 and 60 mg/L schizophyllan significantly increased the apoptotic rate and blocked the cell cycle. In addition, increase in the proportion of cells in G0/G1 phase and decrease in the proportion of S-phase cells were also observed. Overall experimental studies suggest that schizophyllan can significantly inhibit the growth of rat CNS-1 glioma cells, in vitro and induced apoptosis and blocked the cell cycle.

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Published

2015-09-23

How to Cite

Zhou, B., Q. Fu, S.-S. Song, H.-L. Zheng, and Y.-Z. Wei. “Inhibitory Effect of Schizophyllan on Rat Glioma Cells”. Bangladesh Journal of Pharmacology, vol. 10, no. 4, Sept. 2015, pp. 759-64, doi:10.3329/bjp.v10i4.23834.

Issue

Section

Research Articles