Antidiarrheal and antispasmodic activities of Polygonum bistorta rhizomes are mediated predominantly through K+ channels activation

Authors

  • Muhammad Zeeshan Ali Natural Product Research Division, Department of Biological and Biomedical Sciences, The Aga Khan University Medical College, Karachi 74800, Pakistan
  • Khalid Hussain Janbaz Faculty of Pharmacy, Bahauddin Zakariya University, Multan 60800, Pakistan
  • Malik Hassan Mehmood Natural Product Research Division, Department of Biological and Biomedical Sciences, The Aga Khan University Medical College, Karachi 74800, Pakistan
  • Anwar Hassan Gilani Natural Product Research Division, Department of Biological and Biomedical Sciences, The Aga Khan University Medical College, Karachi 74800; Pakistan Council for Science and Technology, G-5/2, Islamabad, Pakistan

DOI:

https://doi.org/10.3329/bjp.v10i3.23714

Keywords:

Antidiarrheal, Antispasmodic, Ca channel antagonist, K channel activation, P. bistorta

Abstract

Polygonum bistorta is a popular medicinal herb used to treat diarrhea. This study provides pharmacological basis to its folk use in diarrhea using in vivo and in vitro assays. Administration of P. bistorta  rhizomes extract to mice offered protection against castor oil-induced diarrhea at 300-1,000 mg/kg and was found safe up to the dose of 5 g/kg. In isolated rabbit jejunum, the extract caused a dose-dependent relaxation of spontaneous and low K+ (25 mM)-induced contractions with weak effect against high K+ (80 mM). In tissues pretreated with glibenclamide or tetraethylammonium chloride (TEA), the relaxant effect of the extract was markedly inhibited by TEA only. While verapamil showed complete relaxation of spontaneous, low K+, low K+ with TEA and high K+-induced contractions. In guinea-pig ileum, mild atropine-sensitive effect was observed. This study indicates that P. bistorta possesses anti-diarrheal and antispasmodic activities mediated predominantly through K+-channels activation along with weak Ca++ antagonist effect.

 

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Author Biographies

Khalid Hussain Janbaz, Faculty of Pharmacy, Bahauddin Zakariya University, Multan 60800, Pakistan

Anwar Hassan Gilani, Natural Product Research Division, Department of Biological and Biomedical Sciences, The Aga Khan University Medical College, Karachi 74800; Pakistan Council for Science and Technology, G-5/2, Islamabad, Pakistan

Professor

Chairman, Pakistan Council for Science and Technology
Google Scholar Citation Index

Published

2015-07-14

How to Cite

Ali, M. Z., K. H. Janbaz, M. H. Mehmood, and A. H. Gilani. “Antidiarrheal and Antispasmodic Activities of Polygonum Bistorta Rhizomes Are Mediated Predominantly through K+ Channels Activation”. Bangladesh Journal of Pharmacology, vol. 10, no. 3, July 2015, pp. 627-34, doi:10.3329/bjp.v10i3.23714.

Issue

Section

Research Articles