Genome size determination of Eclipta alba and Aloe barbadensis

Authors

  • Anggana Roy Department of Molecular Biology and Biotechnology, Tezpur University, Tezpur, Assam 784028, India
  • Yasir Bashir Department of Molecular Biology & Biotechnology, Tezpur University, Tezpur, Assam 784028, India http://orcid.org/0000-0001-7908-6318
  • Irfan Ahmad Rather Microbiome Lab, Department of Applied Microbiology and Biotechnology, School of Biotechnology, Yeungnam University, Gyeongsan si, Gyeongsan 712749, South Korea http://orcid.org/0000-0003-2752-0519
  • Bolin Kumar Konwar Department of Molecular Biology and Biotechnology, Tezpur University, Tezpur, Assam 784028; Nagaland University, HQ-Lumami, Zunheboto, Nagaland 798627, India http://orcid.org/0000-0002-4429-8326

DOI:

https://doi.org/10.3329/bjp.v10i3.23642

Keywords:

Aloe barbadensis, Eclipta alba, Flow cytometry, Genome size

Abstract

There is abundant genetic diversity of pharmacologically important plants around the globe and this pool of genetic variation serves as the base for selection as well as for plant improvement. The major cause of such genetic diversity is the variation in their genetic material, as called genome. In the present study, an attempt was made to determine the genome size of Eclipta alba and Aloe barbadensis by flow cytometry using Pisum sativum as a reference standard. The nuclear DNA was calculated as 8.7 pg for E. alba and 9.0 pg for A. barbadensis. The genome size of E. alba and A. barbadensis was estimated to be 4.27 x 109 bp and 4.42 x 109 bp, respectively. Information on genome size and DNA content of these two pharmacologically important plants would provide a useful tool for future molecular biological investigations.

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Author Biographies

Anggana Roy, Department of Molecular Biology and Biotechnology, Tezpur University, Tezpur, Assam 784028, India

PhD Reseacher

Yasir Bashir, Department of Molecular Biology & Biotechnology, Tezpur University, Tezpur, Assam 784028, India

PhD reseacher

Irfan Ahmad Rather, Microbiome Lab, Department of Applied Microbiology and Biotechnology, School of Biotechnology, Yeungnam University, Gyeongsan si, Gyeongsan 712749, South Korea

Assistant Professor

Google Scholar Citation

 

Bolin Kumar Konwar, Department of Molecular Biology and Biotechnology, Tezpur University, Tezpur, Assam 784028; Nagaland University, HQ-Lumami, Zunheboto, Nagaland 798627, India

Vice Chancellor 

Google Scholar Citation

Published

2015-08-14

How to Cite

Roy, A., Y. Bashir, I. A. Rather, and B. K. Konwar. “Genome Size Determination of Eclipta Alba and Aloe Barbadensis”. Bangladesh Journal of Pharmacology, vol. 10, no. 3, Aug. 2015, pp. 697-02, doi:10.3329/bjp.v10i3.23642.

Issue

Section

Research Articles