Genome size determination of Eclipta alba and Aloe barbadensis

  • Anggana Roy Department of Molecular Biology and Biotechnology, Tezpur University, Tezpur, Assam 784028, India
  • Yasir Bashir Department of Molecular Biology & Biotechnology, Tezpur University, Tezpur, Assam 784028, India http://orcid.org/0000-0001-7908-6318
  • Irfan Ahmad Rather Microbiome Lab, Department of Applied Microbiology and Biotechnology, School of Biotechnology, Yeungnam University, Gyeongsan si, Gyeongsan 712749, South Korea http://orcid.org/0000-0003-2752-0519
  • Bolin Kumar Konwar Department of Molecular Biology and Biotechnology, Tezpur University, Tezpur, Assam 784028; Nagaland University, HQ-Lumami, Zunheboto, Nagaland 798627, India http://orcid.org/0000-0002-4429-8326
Keywords: Aloe barbadensis, Eclipta alba, Flow cytometry, Genome size

Abstract

There is abundant genetic diversity of pharmacologically important plants around the globe and this pool of genetic variation serves as the base for selection as well as for plant improvement. The major cause of such genetic diversity is the variation in their genetic material, as called genome. In the present study, an attempt was made to determine the genome size of Eclipta alba and Aloe barbadensis by flow cytometry using Pisum sativum as a reference standard. The nuclear DNA was calculated as 8.7 pg for E. alba and 9.0 pg for A. barbadensis. The genome size of E. alba and A. barbadensis was estimated to be 4.27 x 109 bp and 4.42 x 109 bp, respectively. Information on genome size and DNA content of these two pharmacologically important plants would provide a useful tool for future molecular biological investigations.

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Author Biographies

Anggana Roy, Department of Molecular Biology and Biotechnology, Tezpur University, Tezpur, Assam 784028, India

PhD Reseacher

Yasir Bashir, Department of Molecular Biology & Biotechnology, Tezpur University, Tezpur, Assam 784028, India

PhD reseacher

Irfan Ahmad Rather, Microbiome Lab, Department of Applied Microbiology and Biotechnology, School of Biotechnology, Yeungnam University, Gyeongsan si, Gyeongsan 712749, South Korea

Assistant Professor

Google Scholar Citation

 

Bolin Kumar Konwar, Department of Molecular Biology and Biotechnology, Tezpur University, Tezpur, Assam 784028; Nagaland University, HQ-Lumami, Zunheboto, Nagaland 798627, India

Vice Chancellor 

Google Scholar Citation

References

Arumugunathan K, Tallury SP, Fraser ML, Bruneau AH, Qu R. Nuclear DNA content of thirteen turfgrass species by flow cytometry. Crop Sci. 1999: 39: 1518-21.

Baranyi M, Greilhuber J. Flow cytometric analysis of genome size variation in cultivated and wild Pisum sativum Fabaceae. Plant Syst Evol. 1995; 194: 23139.

Baranyi M, Greihuber J, Swiecicki WK. Genome size in wild Pisum species. Theor Appl Genet. 1996; 93: 717-21.

Bennett MD, Leitch IJ. Plant genome size research: A field in focus. Ann Bot. 2005a; 95: 1-6.

Bennett MD, Leitch IJ. Nuclear DNA amount in angiosperms: Progress, problems and prospects. Ann Bot. 2005b; 95: 4590.

Datta K, Singh AT, Mukherjee A, Bhat B, Ramesh B, Burman AC. Eclipta alba extract with potential for hair growth promoting activity. J Ethnopharmacol. 2009; 124: 45056.

Dolezel J, Gohde W. Sex determination in dioecious plants Melandrium album and M. rubrum using high-resolution flow cytometry. Cytometry 1998; 19: 10306.

Dolezel J, Greihuber J, Lucretti S, Meister A, Lysak MA, Nardi L, Obermayer R. Plant genome size estimation by flow cytometry: Inter-laboratory comparison. Ann Bot. 1998; 82: 17-26.

Dolezel J, Bartos J. Plant DNA flow cytometry and estimation of nuclear genome size. Ann Bot. 2005; 95: 99-10.

Doleel J, Kubaláková M, Paux E, Barto J, Feuillet C. Chromo-some-based genomics in the cereals. Chrom Res. 2007; 15: 51-66.

Doyle JJ, Doyle JL. A rapid total DNA preparation procedure for fresh plant tissue. Focus. 1990; 12: 13-15.

Gantait S, Debnath S, Ali MN. Genomic profile of the plants with pharmaceutical value. 3 Biotech. 2014; 4: 56378.

Jadhav VM, Thorat RM, Kadam VJ, Salaskar KP. Chemical composition, pharmacological activities of Eclipta alba. J Pharmacol Res. 2009; 28: 112931.

Johnston JS, Bennett MD, Rayburn AL, Galbraith DW, Price HJ. Reference standards for determination of DNA content of plant nuclei. Am J Bot. 1999; 86: 60913.

Kanjilal UN, Bor NL. Flora of Assam. New Delhi, Om Sons Publications, 1939.

Kawata M, Matsumura Y, Oikawa T, Kimizu M, Fukumoto F, Kuroda S. Analysis of DNA extraction buffer components from plant tissue by polymerase chain reaction. Anal Biochem. 2003; 318: 314-17.

Lysak MA, Rostkova JM, Dixon GR, Dolezel J. Limited genome size variation in Sesleria albicans. Ann Bot. 2000; 86: 399-03.

Otto FJ. DAPI staining of fixed cells for high-resolution flow cytometry of nuclear DNA. In: Methods in cell biology. Darzynkiewickz Z, Crissman HA (eds). Vol 33. San Diego, Academic Press, 1990, pp 105-10.

Panda H. Aloe vera: Handbook on cultivation, research finding, products, formulations, extraction and processing. New Delhi, Asia Pacific Business Press Inc., 2003.

Pirttilä AM, Hirsikorpi M, Kämäräinen T, Jaakola L, Hohtola A. DNA isolation methods for medicinal and aromatic plants. Plant Mol Biol Rep. 2001; 19: 73.

Puchooa D. A simple, rapid and efficient method for the extraction of genomic DNA from lychee Litchi chinensis Sonn. Afr J Biotechnol. 2004; 3: 253-55.

Rajeswari R, Umadevi M, Rahale SC, Pushpa R, Selvaven-kadesh S, Sampath Kumar KP, Bhowmik D. Aloe vera: The miracle plant, its medicinal and traditional uses in India. J Pharmacog Phytochem. 2012; 1: 118-24.

Ray A, Bharali P, Konwar BK. Mode of antibacterial activity of eclalbasaponin isolated from Eclipta alba. Appl Biochem Biotechnol. 2013; 171: 200319.

Ray A, Chattopadhyay P, Konwar BK. Anti-alopecic activity of a novel compound from Aloe barbadensis Miller. J Pharmacol Clin Toxicol. 2015; 3: 1048-52.

Richards E, Reichardt M, Rogers S. Preparation of genomic DNA from plant tissue. In: Current protocols in molecular biology. Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, Struhl K (eds). New York, Wiley-InterScience, 1994, pp 2, 3, 5-6.

Roy RK, Thakur M, Dixit VK. Development and evaluation of polyherbal formulation for hair growth-promoting activity. J Cosmet Dermatol. 2007; 6: 108-12.

Kumar KP, Bhowmik D, Chiranjib L, Biswajit M. Aloe vera: A potential herb and its medicinal importance. J Chem Pharm Res. 2010; 2: 21-29.

Towner P. Purification of DNA. In: Essential molecular biology: A practical approach. Brown TA (ed). Vol 1. Oxford, IRL Press, 1991, pp. 4768.

Warude D, Chavan P, Joshi K, Patwardhan B. DNA isolation from fresh and dry plant samples with highly acidic tissue extracts. Plant Mol Biol Rep. 2003; 21: 467.

Published
2015-08-14
How to Cite
Roy, A., Y. Bashir, I. Rather, and B. Konwar. “Genome Size Determination of Eclipta Alba and Aloe Barbadensis”. Bangladesh Journal of Pharmacology, Vol. 10, no. 3, Aug. 2015, pp. 697-02, doi:10.3329/bjp.v10i3.23642.
Section
Research Articles