Nigella sativa improves the carbon tetrachloride-induced lung damage in rats through repression of erk/akt pathway
The objective of this study was to examine whether Nigella sativa plays a protective role against the damage in the lung by administering carbon tetra-chloride (CCl4) to rats. Male Wistar albino (n=28, 8 weeks old) rats were divided into 4 groups: a) negative control: Normal water consuming group to which no CCl4 and N. sativa was administered; b) Positive control: Normal water consuming group to which no CCl4 was administered but N. sativa was administered; c) CCl4 Group: Normal water consuming and group to which CCl4 was administered (1.5 mL/kg, ip); d) N. sativa plus CCl4 group: CCl4 and N. sativa administered group (1.5 mL/kg, ip). Caspase-3, caspase -9, erk, akt protein syntheses were examined via Western blotting. Malondialdehyde determination in lung tissue was made using spectrophotometer. As a results, malondialdehyde amount was decreased in the CCl4 plus N. sativa group in comparison to CCl4 group whereas caspase-3, caspase-9 was increased and erk, akt had decreased. These results show that N. sativa protects the lung against oxidative damage.
Ahmad A, Husain A, Mujeeb M, Khan SA, Najmi AK, Siddique NA, Damanhouri ZA, Anwar F. A review on therapeutic potential of Nigella sativa: A miracle herb. Asian Pac J Trop Biomed. 2013; 3: 337-52.
Al-Dbass AM, Al-Daihan SK, Bhat RS. Agaricus blazei murill as an efficient hepatoprotective and antioxidant agent against CCl4-induced liver injury in rats. Saudi J Biol Sci. 2012; 19: 303-09.
Aqel M, Shaheen R. Effects of the volatile oil of Nigella sativa seeds on the uterine smooth muscle of rat and guinea pig. J Ethnopharmacol. 1996; 52: 23-26.
Aslan A. The effects of different essential fruit juice and their combination on Saccharomyces cerevisiae cell growth. Progr Nutr. 2015; 17: 36-40.
Aslan A, Can MI, Boydak D, Anti-oxidant Effects of pomegranate juice on Saccharomyces cerevisiae Cell Growth. Afr J Tradit Comple-ment Altern Med. 2014; 11: 14-18.
Aslan A, Can MI. Milk thistle impedes the development of carbon tetrachloride-induced liver damage in rats through suppression of bcl-2 and regulating caspase pathway. Life Sci. 2014; 117: 13-18.
Bahcecioglu IH, Koca SS, Poyrazoglu OK, Yaln?z M, Ozercan IH, Ustundag B, Sahin K, Dagl? AF, Is?k A. Hepatoprotective effect of infliximab, and anti-tnf-? agent, on carbon tetrachloride-induced hepatic fibrosis. Inflammation. 2008; 31: 215-21.
Harzallah H J, Kouidhi B, Flamini G, Bakhrouf A, Mahjoub T. Chemical composition, antimicrobial potential against cariogenic bacteria and cytotoxic activity of Tunisian Nigella sativa essential oil and thymoquinone. Food Chem. 2011; 129: 1469-74.
Hsu J, Kan W, Hsieh C, Choudhry MA, Bland KI, Chaudry IH. Role of extracellular signal-regulated protein kinase (erk) in 17b-estradiol mediated attenuation of lung injury after trauma-hemorrhage. Surgery. 2009; 145: 226-34.
Jayaraman G, Priyadharsini JV, Rajiv R, Anandan B, Raghun and Hakumar S, Shabnam J, Sambantham S, Paramasivam A. Anti-cancer effects of thymoquinone in mouse neuro-blastoma (neuro-2a) cells through caspase-3 activation with down-regulation of xiap,. Toxicol Lett. 2012; 213: 151-59.
Kanter M, Co?kun O, Gürel A. Effect of Nigella sativa on cadmium-induced oxidative stress in the blood of rats. Biol Trace Elem Res. 2005; 107: 277-87.
Khalife R, Stephany E, Tarras O, Hodroj MH, Rizk S. Anti proliferative and proapoptotic effects of topotecan in combination with thymoquinone on acute myelogenous leukemia, clinical lymphoma. Myeloma Leuk. 2014; 14: 46-55.
Korsmeyer SJ. Bcl-2 gene family and the regulation of programmed cell death. Cancer Res. 1999; 59: 1693-700.
Krishnan N, Muthukrishnan S. Effect of Nigella sativa seed extract on carbon tetrachloride-induced hepatotoxicity in rats. J Acute Med. 2012; 2: 107-13.
Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970; 227: 680-85.
Li R, Chen B, Wang G, Yu B, Ren G, Ni G. Effects of mechanical strain on oxygen free radical system in bone marrow mesenchymal stem cells from children. Injury 2011; 42: 753-57.
Lu Z, Xu S. ERK1/2 map kinases in cell survival and apop-tosis. IUBMB Life. 2006; 58: 621-31.
Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem. 1979; 95: 351-58.
Sahin A, Yener Z, Dagoglu G, Dede S, Oto G, Alkan M The effect of Nigella sativa and vit. E + selenium in the prevention of liver necrosis experimentally induced with carbon tetrachloride (CCl4) in rats. Turk J Vet Anim Sci. 2003; 27: 141-52.
Sahin K, Tuzcu M, Gencoglu H, Dogukan A, Timurkan M, Sahin N, Aslan A, Kucuk O. Epigallocatechin-3-gallate activates nrf2/ho-1 signaling pathway in cisplatin-induced nephrotoxicity in rats. Life Sci. 2010; 87: 240-45.
Sahin N, Akdemir F, Orhan C, Aslan A, Agca CA, Gencoglu H, Ulas M, Tuzcu M, Viyaja J, Komorowski JR, Sahin K. A novel nutritional supplement containing chromium picolinate, phosphatidylserine, docosahexaenoic acid, and boron activates the antioxidant pathway nrf2/ho-1 and protects the brain against oxidative stress in high-fat-fed rats. Nutr Neurosci. 2012; 15: 42-47.
Shaker E, Mahmoud H, Mnaa S. Silymarin, the antioxidant component, and Silybum marianum extracts prevent liver damage. Food Chem Toxicol. 2010; 48: 803-06.
Tayman C, Cekmez F, Kafa IM, Canpolat FE, Cetinkaya M, Tonbul A, Uysal S, Tunc T, Sar?c? SU. Protective effects of Nigella sativa oil in hyperoxia-induced lung injury. Archivos De Bronconeumologia. 2013; 49: 15-21.
Tuzcu M, Aslan A, Tuzcu Z, Yabas M, Bahcecioglu IH, Ozercan IH, Kucuk O, Sahin K. Tomato powder impedes the development of azoxymethane-induced colorectal cancer in rats through suppression of COX-2 expression via NF-?B and regulating Nrf2/HO-1 pathway. Mol Nutr Food Res. 2012; 56: 1477-81.
Ustundag B, Bahcecioglu I, Sahin K, Gulcu F, Duzgun S, Ozercan IH, Gursu MF. Effects of soy isoflavones on carbon tetrachloride (CCl4)-induced liver damage and on the level of plasma paraoxonase with arylesterase activities. Firat Univ Med J Health. 2005; 19: 263-71.
Vuda M, DSouza R, Upadhya S, Kumar V, Rao N, Kumar V, Boillat C, Mungli P. Hepatoprotective and antioxidant activity of aqueous extract of Hybanthus enneaspermus against CCl4-induced liver injury in rats. Exp Toxicol Pathol. 2012; 64: 855-59.
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