Nigella sativa improves the carbon tetrachloride-induced lung damage in rats through repression of erk/akt pathway

  • Abdullah Aslan Department of Biology, Faculty of Science, Firat University, Elazig, Turkey
  • Didem Boydak Department of Biology, Faculty of Science, Firat University, Elazig, Turkey
  • Muhammed Ismail Can Department of Biotechnology and Molecular Biology, Aksaray University, Aksaray, Turkey
  • Tuncay Kuloglu Department of Histology and Embryology, Faculty of Medicine, Firat University, Elazig, Turkey
Keywords: Nigella sativa, Lung damage, Caspase-3, Caspase-9, erk, akt.


The objective of this study was to examine whether Nigella sativa plays a protective role against the damage in the lung by administering carbon tetra-chloride (CCl4) to rats. Male Wistar albino (n=28, 8 weeks old) rats were divided into 4 groups: a) negative control: Normal water consuming group to which no CCl4 and N. sativa was administered; b) Positive control: Normal water consuming group to which no CCl4 was administered but N. sativa was administered; c) CCl4 Group: Normal water consuming and group to which CCl4 was administered (1.5 mL/kg, ip); d) N. sativa plus CCl4   group: CCl4 and N. sativa administered group (1.5 mL/kg, ip). Caspase-3, caspase -9, erk, akt protein syntheses were examined via Western blotting. Malondialdehyde determination in lung tissue was made using spectrophotometer. As a results, malondialdehyde amount was decreased in the CCl4 plus N. sativa group in comparison to CCl4 group whereas caspase-3, caspase-9 was increased and erk, akt had decreased. These results show that N. sativa protects the lung against oxidative damage.



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How to Cite
Aslan, A., D. Boydak, M. I. Can, and T. Kuloglu. “Nigella Sativa Improves the Carbon Tetrachloride-Induced Lung Damage in Rats through Repression of Erk/Akt Pathway”. Bangladesh Journal of Pharmacology, Vol. 10, no. 3, July 2015, pp. 654-9, doi:10.3329/bjp.v10i3.23486.
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