GC/MS analysis, free radical scavenging, anticancer and beta-glucuronidase inhibitory activities of Trillium govanianum rhizome

Authors

  • Shafiq ur Rahman Department of Pharmacy, University of Peshawar, PeshawarDepartment of Pharmacy, ShaheedBenazir Bhutto University, Sheringal, Dir UpperH.E.J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi, Pakistan
  • Muhammad Ismail Department of Pharmacy, University of Peshawar, Peshawar, Pakistan
  • Muhammad Raza Shah H.E.J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi, Pakistan
  • Marcello Iriti Department of Agricultural and Environmental Sciences, Milan State University, Milan, Italy
  • Muhammad Shahid Department of Pharmacy, University of Peshawar, Peshawar, Pakistan

DOI:

https://doi.org/10.3329/bjp.v10i3.23446

Keywords:

Anticancer, Antioxidant, GC-MS analysis, ?-Glucuronidase, Trillium govanianum

Abstract

The current study aims to investigate the phytochemical constituents, antioxidant, β-glucuronidase inhibitory and anticancer activities of Trillium govanianum rhizome. Phytochemical screening revealed the presence of steroidal glycosides, saponins, sterols, flavonoids and carbohydrates. GC/MS analyses of n-hexane fraction identified 12 compounds, including 70% unsaturated and 30% saturated fatty acids. Higher free radical scavenging capacity was observed in n-hexane and chloroform fraction compared with the other fractions. Based on IC50 (μg/mL) values, antiproliferative activity on HeLa cells was observed for  chloroform (0.8 ± 0.2), ethyl acetate (1.4 ± 0.1) and butanol (1.6 ± 0.3) fractions by comparison to anticancer drug doxorubicin (0.3 ± 0.0). Similarly, all fractions exhibited cytotoxicity on PC-3 cells. Moreover, the methanol extract (IC50: 140.8 ± 3.8) and butanol fraction (196.2 ± 1.9) exhibited a moderate level of β-glucuronidase inhibitory activity. These findings may validate the folkloric uses of T. govanianum rhizome in cancer management, and can be a promising candidate as an anticancer agent.

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Published

2015-07-05

How to Cite

Rahman, S. ur, M. Ismail, M. R. Shah, M. Iriti, and M. Shahid. “GC/MS Analysis, Free Radical Scavenging, Anticancer and Beta-Glucuronidase Inhibitory Activities of Trillium Govanianum Rhizome”. Bangladesh Journal of Pharmacology, vol. 10, no. 3, July 2015, pp. 577-83, doi:10.3329/bjp.v10i3.23446.

Issue

Section

Research Articles