Prevention of secretory diarrhea by ethanol extract of Bistortae rhizoma through inhibition of chloride channel

Authors

  • Bo Yu Jilin Provincial Key Laboratory on Molecular and Chemical Genetics, The Second Hospital of Jilin University, Changchun 130041, China http://orcid.org/0000-0002-5976-6364
  • Yu Jiang School of Life Sciences, Liaoning Provincial Key Laboratory of Biotechnology and Drug Discovery, Liaoning Normal University, Dalian 116029, China
  • Yue Liu School of Life Sciences, Liaoning Provincial Key Laboratory of Biotechnology and Drug Discovery, Liaoning Normal University, Dalian 116029, China
  • Tonghui Ma Jilin Provincial Key Laboratory on Molecular and Chemical Genetics, The Second Hospital of Jilin University, Changchun 130041; College of Basic Medical Sciences, Dalian Medical University, Dalian 116044, China
  • Hong Yang School of Life Sciences, Liaoning Provincial Key Laboratory of Biotechnology and Drug Discovery, Liaoning Normal University, Dalian 116029, China

DOI:

https://doi.org/10.3329/bjp.v10i3.23260

Keywords:

Bistortae Rhizoma, Chloride channel, Diarrhea

Abstract

Inhibition of cystic fibrosis transmembrane conductance regulator (CFTR) and Ca2+-activated Cl- channel (CaCC) represents an attractive approach for the treatment of secretory diarrhea. The aim of the study is to investigate the molecular basis of the anti-diarrheal effect of traditional Chinese herbal anti-diarrheal medicine Bistortae rhizoma. Fluorescence quenching assay indicated that the 40% methanol /water fraction (D5) dose-dependently inhibited both CFTR and CaCC function in transfected Fischer rat thyroid (FRT) cells. Ex vivo studies indicated that D5 inhibited both forskolin (FSK)-activated CFTR current and CCh-induced CaCC current in rat colonic mucosa. In the mouse closed-loop model, intraluminal application of D5 (200 µg/mL) significantly reduced cholera toxin-stimulated fluid secretion. In the intestinal motility model, D5 significantly delayed intestinal peristalsis in mice. Our research suggests that CFTR and CaCC-mediated intestinal epithelial Cl- secretion inhibiting and gastrointestinal motility delaying may account for the anti-diarrheal activity of B. rhizoma.  

 

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Published

2015-07-01

How to Cite

Yu, B., Y. Jiang, Y. Liu, T. Ma, and H. Yang. “Prevention of Secretory Diarrhea by Ethanol Extract of Bistortae Rhizoma through Inhibition of Chloride Channel”. Bangladesh Journal of Pharmacology, vol. 10, no. 3, July 2015, pp. 533-42, doi:10.3329/bjp.v10i3.23260.

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Section

Research Articles