Induction of cell death in prostate cancer cells by escopoletin, a promising treatment strategy


  • Da Chen Department of Urology, Beijing Shijitan Hospital, Capital Medical University. Beijing 100038
  • Xiao-Yi Zhang Department of Urology, The Second Artillery General Hospital PLA, Beijing 100088
  • Fa-Zhu Zheng Department of Urology Handan Peoples? Hospital, Handan 056001
  • Hai-Tao Wang Department of Urology, Beijing Shijitan Hospital, Capital Medical University. Beijing 100038
  • Jian-Liang Cai Department of Urology, Beijing Shijitan Hospital, Capital Medical University. Beijing 100038
  • Ming Xia Department of Urology, Beijing Shijitan Hospital, Capital Medical University. Beijing 100038



Antioxidant, Cancer, Cell viability, Escopoletin, Prostate


Escopoletin, a phenolic compound belonging to anthocyanin family shows promising antioxidant activities. In the present study, anti-cancer effects of escopoletin treatment in DU145 cells were investigated. The sulphorhodamine-B staining and annexin V and propidium iodide were respectively used for the analysis of cell viability and death. The results revealed a significantly higher cytotoxicity by escopoletin that caused cell death in DU145 cells. Escopoletin treatment in DU145 cells markedly inhibited cell growth through non-apoptotic cell death and induced significant reactive oxygen species (ROS) production. It also induced G1 cell cycle arrest and cyclin D1 accumulation through the enhanced expression of p21. However, the effect of escopoletin on DU145 cells was reversed by pretreatment with glutathione antioxidant. This suggests that escopoletin induced generation of ROS is responsible for the increased cytotoxicity in DU145 cells. Thus, escopoletin exhibits potential therapeutic efficacy for the treatment of prostate cancer.



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Abbas T, Dutta A. p21 in cancer: Intricate networks and multiple activities. Nat Rev Cancer. 2009; 9: 400?14.

Bravo L. Polyphenols: Chemistry, dietary sources, metabolism and nutritional significance. Nutr Rev. 1998; 56: 317-33.

Diamandis EP. Prostate-specific antigen: Its usefulness in clinical medicine. Trends Endocrinol Metab. 1998; 9: 310-16.

Di Carlo G, Mascolo N, Izzo AA, Capasso F. Flavonoids: Old and new aspects of a class of natural therapeutic drugs. Life Sci. 1999; 65: 337-53.

Di Stefano A, Frosali S, Leonini A, Ettorre A, Priora R, Di Simplicio FC, Di Simplicio P. GSH depletion, protein S?glutathionylation and mitochondrial transmembrane potential hyperpolarization are early events in initiation of cell death induced by a mixture of isothiazolinones in HL60 cells. Biochim Biophys Acta. 2006; 1763: 214-25.

Gro¨nberg, H. Prostate cancer epidemiology. Lancet 2003; 361: 859-64.

Hood JE, Jenkins JW, Milatovic D, Rongzhu L, Aschner M. Mefloquine induces oxidative stress and neurodegeneration in primary rat cortical neurons. Neurotoxicology 2010; 31: 518-23.

Montoro P, Braca A, Pizza C, De Tommasi N. Structureantioxidant activity relationships of flavonoids isolated from different plant species. Food Chem. 2005; 92: 349-55.

Sardana G, Dowell B, Diamandis EP. Emerging biomarkers for the diagnosis and prognosis of prostate cancer. Clin Chem. 2008; 54: 1951-60.

van Acker SABE, van den Berg DJ, Tromp MNJL, Griffioen DH, van Bennekom WP, van der Vijgh WJF, Bast A. Structural aspects of antioxidant activity of flavonoids. Free Radic Biol Med. 1996; 20: 331-42.

Velioglu YS, Mazza G, Gao L, Oomah BD. Antioxidant activity and total phenolics in selected fruits, vegetables and grain products. J Agric Food Chem. 1998; 46: 4113-17.



How to Cite

Chen, D., X.-Y. Zhang, F.-Z. Zheng, H.-T. Wang, J.-L. Cai, and M. Xia. “Induction of Cell Death in Prostate Cancer Cells by Escopoletin, a Promising Treatment Strategy”. Bangladesh Journal of Pharmacology, vol. 10, no. 3, July 2015, pp. 500-4, doi:10.3329/bjp.v10i3.23095.



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