Induction of cell death in prostate cancer cells by escopoletin, a promising treatment strategy

Authors

  • Da Chen Department of Urology, Beijing Shijitan Hospital, Capital Medical University. Beijing 100038
  • Xiao-Yi Zhang Department of Urology, The Second Artillery General Hospital PLA, Beijing 100088
  • Fa-Zhu Zheng Department of Urology Handan Peoples? Hospital, Handan 056001
  • Hai-Tao Wang Department of Urology, Beijing Shijitan Hospital, Capital Medical University. Beijing 100038
  • Jian-Liang Cai Department of Urology, Beijing Shijitan Hospital, Capital Medical University. Beijing 100038
  • Ming Xia Department of Urology, Beijing Shijitan Hospital, Capital Medical University. Beijing 100038

DOI:

https://doi.org/10.3329/bjp.v10i3.23095

Keywords:

Antioxidant, Cancer, Cell viability, Escopoletin, Prostate

Abstract

Escopoletin, a phenolic compound belonging to anthocyanin family shows promising antioxidant activities. In the present study, anti-cancer effects of escopoletin treatment in DU145 cells were investigated. The sulphorhodamine-B staining and annexin V and propidium iodide were respectively used for the analysis of cell viability and death. The results revealed a significantly higher cytotoxicity by escopoletin that caused cell death in DU145 cells. Escopoletin treatment in DU145 cells markedly inhibited cell growth through non-apoptotic cell death and induced significant reactive oxygen species (ROS) production. It also induced G1 cell cycle arrest and cyclin D1 accumulation through the enhanced expression of p21. However, the effect of escopoletin on DU145 cells was reversed by pretreatment with glutathione antioxidant. This suggests that escopoletin induced generation of ROS is responsible for the increased cytotoxicity in DU145 cells. Thus, escopoletin exhibits potential therapeutic efficacy for the treatment of prostate cancer.

 

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Published

2015-07-01

How to Cite

Chen, D., X.-Y. Zhang, F.-Z. Zheng, H.-T. Wang, J.-L. Cai, and M. Xia. “Induction of Cell Death in Prostate Cancer Cells by Escopoletin, a Promising Treatment Strategy”. Bangladesh Journal of Pharmacology, vol. 10, no. 3, July 2015, pp. 500-4, doi:10.3329/bjp.v10i3.23095.

Issue

Section

Research Articles