Screening of Ranunculus sceleratus for enzyme inhibition, antibacterial and antioxidant activities
Keywords:Cholinesterase, DPPH radical, ?-Glucosidase, Lipoxygenase, MBC, MIC
Enzyme inhibition potential of various fractions of Ranunculus sceleratus was checked against ?-glucosidase, butyrylcholinesterase, acetylcholinesterase and lipoxygenase enzymes. n-Butanol fraction showed very good activity (77.5 ± 1.0% inhibition at 0.1 mg/mL) against ?-glucosidase. Its IC50 value was 35.7 ± 1.0 µg/mL comparable to quercetin (IC50 value 16.5 ± 0.4 µg/mL). Antibacterial activity was checked against five bacterial strains by 96-wells microplate assay using ciprofloxacin, a standard antibiotic. Chloroform, ethyl acetate, n-butanol and aqueous fractions showed excellent activity against Pseudomonas aeruginosa, (MIC at 7.1, 7.8, 5.6 and 5.3 respectively), which is greater than standard antibiotic ciprofloxacin (MIC 10.0). The antioxidant potential of all the fractions was evaluated. Ethyl acetate soluble fraction exhibited highest percent inhibition of DPPH radical as compared to other fractions. It showed 80.9 ± 1.2% inhibition of DPPH radical at a concentration of 30 µg/mL. These results suggest that R. sceleratus is a valuable herb, which inhibits the oxidative stress mechanism that lead to degenerative diseases.
How to Cite
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).