Phytochemical, antimicrobial and cytotoxic evaluation of Indigofera serpentinicola

  • Charmaine Kahiya University of Zimbabwe, Biological Sciences Department, P.O. Box MP 167, Mt Pleasant, Harare
  • Clemence Zimudzi University of Zimbabwe, Biological Sciences Department, P.O. Box MP 167, Mt Pleasant, Harare
Keywords: Antimicrobial, Cytotoxicity, Indigofera serpentinicola, Phytochemical

Abstract

Methanol and aqueous root extracts of Indigofera serpentinicola were investigated for their phytochemical, antimicrobial and cytotoxic properties. Phytochemical analysis showed the presence of alkaloids, saponins, tanins, terpenoids, cardiac glycosides, phenols, reducing sugars oils and fats in both extracts. Flavanoids were only detected in the methanolic extract. Antimicrobial activity was evaluated using the Kirby-Bauer disk diffusion method. The extracts showed low activity against Staphylococus aureus, Cornybacterium diptheriae, Pseudomonas aeruginosa and Salmonella enterica and no activity against Escherichia coli. The diameters zones of inhibition ranged between 3-10 mm. The aqueous extract had higher activity showing zones of inhibition of 10 mm against S. enterica. Brine shrimp lethality test showed LC50 values which ranged from 0.079-0.158 mg/mL, showing that the extracts were highly toxic. 

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Author Biography

Clemence Zimudzi, University of Zimbabwe, Biological Sciences Department, P.O. Box MP 167, Mt Pleasant, Harare

Department of Biological Sciences

Senior Lecturer

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Published
2015-03-02
How to Cite
Kahiya, C., and C. Zimudzi. “Phytochemical, Antimicrobial and Cytotoxic Evaluation of Indigofera Serpentinicola”. Bangladesh Journal of Pharmacology, Vol. 10, no. 1, Mar. 2015, pp. 166-72, doi:10.3329/bjp.v10i1.21467.
Section
Research Articles