Effect of crude saponins from Gaultheria trichophylla extract on growth inhibition in human colorectal cancer cells

Authors

  • Fiaz Alam Department of Pharmacy, COMSATS Institute of Information Technology, Abbottabad
  • Qazi Najumus Saqib Department of Pharmacy, COMSATS Institute of Information Technology, Abbottabad
  • Abdul Waheed Wolfson Cell Science Centre, Faculty of Health & Medical Sciences, University of Surrey, Guildford

DOI:

https://doi.org/10.3329/bjp.v10i1.21462

Keywords:

Apoptosis, G. trichophylla, MTT, Saponins

Abstract

The genus Gaultheria also comprised of species with reported cytotoxic activities. Current research work  was carried out to evaluate G. trichophylla crude extract and respective saponins fraction against human colorectal cancer cell line (Caco-2) based on cell viability assays. Caco-2 cells treated with the crude extract showed significant growth  inhibition (p< 0.001) in a dose dependent manner with apparent IC50 value of 200 ?g/mL and 100 ?g/mL in MTT and NRU assays respectively. The fractioned crude saponins showed an enhanced response and inhibited the growth of Caco-2 by 93.6 and 97.4% in MTT and NRU assays respectively, with compared to actinomycin-D (65%). The DAPI staining of cell treated with crude saponins observed under confocal microscope showed shrunken nuclei with apparent nuclear fragmentation and chromatin condensation indicating apoptosis mode of cell death. The study exhibited that the G. Trichophylla saponins induced apoptosis of Caco-2 cell lines. This study provides new evidences to further explore this plant for the novel targets in anticancer drug development.

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Author Biographies

Fiaz Alam, Department of Pharmacy, COMSATS Institute of Information Technology, Abbottabad

Assistant Professor

Qazi Najumus Saqib, Department of Pharmacy, COMSATS Institute of Information Technology, Abbottabad

Professor

Published

2015-03-01

How to Cite

Alam, F., Q. N. Saqib, and A. Waheed. “Effect of Crude Saponins from Gaultheria Trichophylla Extract on Growth Inhibition in Human Colorectal Cancer Cells”. Bangladesh Journal of Pharmacology, vol. 10, no. 1, Mar. 2015, pp. 160-5, doi:10.3329/bjp.v10i1.21462.

Issue

Section

Research Articles