Extract of Brassica rapa suppresses ovalbumin-induced asthma by down-regulating NF-kappaB and p38 MAPK activity

  • Yun-Lei Dai Department of Pediatrics, The First Affiliated Hospital of Henan University of TCM, Zhengzhou 450000
  • Gui-Lian Shao Department of Pediatrics, Weihai Meternal and Child Health Hospital, Weihai 264200
  • Fang Wang Department of Pediatrics, Zhengzhou Central Hospital Affiliated to Zhengzhou University, Zhengzhou 450007
Keywords: Asthma, Brassica rapa chinensis, NF-?B, p38MAPK, Th2 cytokines

Abstract

Asthma is one of the most common chronic inflammatory diseases characterized by airway infiltration of inflammatory cells inducing hyper-responsiveness. Due to the inefficacy of currently used drugs to definitely cure asthma, there is an insistent need for identification of novel therapies. In the present research, efficiency of Brassica rapa extract in asthma-induced mouse model was investigated. Asthma was induced in mice by ovalbumin induction. The extract (125-500 mg/kg) effectively reduced ovalbumin-specific IgE in serum and levels of interleukins (IL): IL-4, IL-5 and IL-13 in bronchoalveolar lavage fluid (BALF). B. rapa extract inhibited inflammatory cell infiltration in BALF and also improved lung compliance and airway resistance. The extract caused marked reduction in the expression levels of phosphorylated ERK, p38, I?B-? and NF-?B, however dose-dependent activation of JNK was observed. The extract of B. rapa thus was effective in suppressing ovalbumin-induced asthma.

 

Downloads

Download data is not yet available.
Abstract
1191
Download
391 Read
273

References

Akira S, Takeda K, Kaisho T. Toll-like receptors: Critical proteins linking innate and acquired immunity. Nat Immunol. 2001; 2: 675-80.

Alcorn JF, Ckless K, Brown AL, Guala AS, Kolls JK, Poynter ME, Irvin CG, van der Vliet A, Janssen-Heininger YM. Strain dependent activation of NF-kappaB in the airway epithelium and its role in allergic airway inflammation. Am J Physiol Lung Cell Mol Physiol. 2010; 298: L57-66.

Bao Z, Lim S, Liao W, Lin Y, Thiemermann C, Leung BP, Wong WS. Glycogen synthase kinase-3 beta inhibition attenuates asthma in mice. Am J Respir Crit Care Med. 2007; 176: 431-38.

Berend N, Salome CM, King GG. Mechanisms of airway hyperresponsiveness in asthma. Respirology 2008; 13: 624-31.

Bisset LR, Schmid-Grendelmeier P. Chemokines and their receptors in the pathogenesis of allergic asthma: Progress and perspective. Curr Opin Pulm Med. 2005; 11: 35-42.

Boulanger D, Bureau F, Melotte D, Mainil J, Lekeux P. Increased nuclear factor kappaB activity in milk cells of mastitis-affected cows. J Dairy Sci. 2003; 86: 1259-67.

Busse WW, Lemanske Jr RF. Asthma. N Engl J Med. 2001; 344: 350-62.

Busse WW, Coffman RL, Gelfand EW, Kay AB, Rosenwasser LJ. Mechanisms of persistent airway inflammation in asthma: A role for T cells and T-cell products. Am J Respir Crit Care Med. 1995; 152: 388-93.

Chang L, Karin M. Mammalian MAP kinase signaling cascades. Nature 2001; 410: 37.

Chio YH, Park HY. Anti-inflammatory effects of spermidine in lipopolysaccharide stimulated BV2 microglial cells. J Biomed Sci. 2012; 19: 31.

Choi IW, Kim DK, Ko HM, Lee HK. Administration of antisense phosphorothioate oligonucleotide to the p65 subunit of NF-?B inhibits established asthmatic reaction in mice. Int Immunopharmacol. 2004; 4: 1817-28.

Cohn L, Tepper JS, Bottomly K. IL-4-independent induction of airway hyperresponsiveness by Th2, but not Th1, cells. J Immunol. 1998; 161: 3813-16.

Das J, Chen CH, Yang L, Cohn L, Ray P, Ray A. A critical role for NF-?B in GATA3 expression and Th2 differentiation in allergic airway inflammation. Nat Immunol. 2001; 2: 45-50.

Desai D, Brightling C. Cytokines and cytokine-specific therapy in asthma. Adv Clin Chem. 2012; 57: 57-97.

Duan W, Chan JH, McKay K, Crosby JR, Choo HH, Leung BP, Karras JG, Wong WS. Inhaled p38 alpha mitogen-activated protein kinase antisense oligonucleotide attenuates asthma in mice. Am J Respir Crit Care Med. 2005; 171: 571-78.

Elias JA, Lee CG, Zheng T, Ma B, Homer RJ, Zhu Z. New insights into the pathogenesis of asthma. J Clin Invest. 2003; 111: 291-97.

Fish SC, Donaldson DD, Goldman SJ, Williams CM, Kasaian MT. IgE generation and mast cell effector function in mice deficient in IL-4 and IL-13. J Immunol 2005; 174: 7716-7724.

Gleich GJ. Mechanisms of eosinophil-associated inflammation. J Allergy Clin Immunol. 2000; 105: 651-63.

Griswold DE, Young PR. Pharmacology of cytokine suppressive anti-inflammatory drug binding protein (CSBP), a novel stress-induced kinase. Pharmacol Commun. 1996; 7: 323-29.

Grunig G, Warnock M, Wakil AE, Venkayya R, Brombacher F, Rennick DM, Sheppard D, Mohrs M, Donaldson DD, Locksley RM, Corry DB. Requirement for IL-13 indepen-dently of IL-4 in experimental asthma. Science 1998; 282: 2261-63.

Hendeles L, Soreness CA. Anti-immunoglobulin E therapy with omalizumab for asthma. Ann Pharmacother. 2007; 41: 1397-410.

Herrick CA, Bottomly K. To respond or not to respond: T cells in allergic asthma. Nat Rev Immunol. 2003; 3: 1.

Hocaoglu AB, Karaman O, Erge DO, Erbil G, Yilmaz O, Bagriyanik A, Uzuner N. Glycyrrhizin and long-term histo-pathologic changes in a murine model of asthma. Curr Thera Res. 2011; 72: 250-61.

Jabara HH, Geha RS. Jun N-terminal kinase is essential for CD40-mediated IgE class switching in B cells. J Allergy Clin Immunol. 2005; 115: 856-63.

Janson C. The importance of airway remodeling in the natural course of asthma. Clin Respir J. 2010; 4: 28-34.

Juneja L, Parmar HS. Ovalbumin induced allergic rhinitis and development of prediabetes to rats: possible role of Th2 cytokines. Inflamm Allergy Drug Targets. 2013; 12: 199-205.

Kang NI, Yoon HY, Lee YR, Won M, Chung MJ, Park JW, Hur GM, Lee HK, Park BH. A20 attenuates allergic airway inflammation in mice. J Immunol. 2009; 183: 1488-95.

Kips JC, Tournoy KG, Pauwels RA. New antiasthma therapies: Suppression of the effect of interleukin (IL)-4 and IL-5. Eur Respir J. 2001; 17: 499-506.

Kumar A, Lnu S, Malya R, Barron D, Moore J, Corry DB, Boriek AM. Mechanical stretch activates nuclear factor-kappaB, activator protein-1, and mitogen-activated protein kinases in lung parenchyma: implications in asthma. FASEB J. 2003; 17: 1800.

Larche M, Robinson DS, Kay AB. The role of T lymphocytes in the pathogenesis of asthma. J Allergy Clin Immunol. 2002; 111: 450-63.

Leigh R, Ellis R, Wattie JN, Hirota JA, Matthaei KI, Foster PS, O'Byrne PM, Inman MD. Type 2 cytokines in the pathogenesis of sustained airway dysfunction and airway remodeling in mice. Am J Respir Crit Care Med. 2004; 169: 860-67.

Li XM, Brown L. Efficacy and mechanisms of action of Traditional Chinese Medicines for treating asthma and allergy. J Allergy Clin Immunol. 2009; 123: 297-308.

Li XM. Complementary and alternative medicine in pediatric allergic disorders. Curr Opin Allergy Clin Immunol. 2009; 9: 161-67.

Li X M. Treatment of asthma and food allergy with herbal interventions from Traditional Chinese Medicine. Mt Sinai J Med. 2011; 78: 697-716.

Ngoc PL, Gold DR, Tzianabos AO, Weiss ST, Celedon JC. Cytokines, allergy, and asthma. Curr Opin Allergy Clin Immunol. 2005; 5: 161-66.

Oh SW, Pae CI, Lee DK, Jones F, Chiang GK, Kim HO, Moon SH, Cao B, Ogbu C, Jeong KW, Kozu G, Nakanishi H, Kahn M, Chi EY, Henderson Jr WR. Tryptase inhibition blocks airway inflammation in a mouse asthma model. J Immunol. 2002; 168: 1992-2000.

Podsedek A. Natural antioxidants and antioxidant capacity of Brassica vegetables: A review. LWT-Food Sci Technol. 2007; 40: 1-11.

Powers SK, DeRuisseau KC, Quindry J, Hamilton KL. Dietary antioxidants and exercice. J Sports Sci. 2004; 22: 81-94.

Poynter ME, Irvin CG, Janssen-Heininger YM. Rapid activation of nuclear factor-kappaB in airway epithelium in a murine model of allergic airway inflammation. Am J Pathol. 2002; 160: 1325-34.

Shahzad M, Yang X, Raza Asim MB, Sun Q, Han Y, Zhang F, Cao Y, Lu S. Black seed oil ameliorates allergic airway inflammation by inhibiting T-cell proliferation in rats. Pulmonary Pharmacol Ther. 2009; 22: 37-43.

Siebenlist U, Brown K, Claudio E. Control of lymphocyte development by NFkB. Nat Rev Immunol. 2005; 5: 435-45.

Teran LM, Mochizuki M, Bartels J, Valencia EL, Nakajima T, Hirai K, Schroder JM. Th1- and Th2-type cytokines regulate the expression and production of eotaxin and RANTES by human lung fibroblasts. Am J Respir Cell Mol Biol. 1999; 20: 1044-49.

Underwood DC, Osborn RR, Kotzer CJ, Adams JL, Lee JC, Webb EF, Carpenter DC, Bochnowicz S, Thomas HC, Hay DW, Griswold DE. SB 239063, a potent p38 MAP kinase inhibitor, reduces inflammatory cytokine production, airways eosinophil infiltration, and persistence. J Pharmacol Exp Ther. 2000; 293: 281-88.

Walsh GM. Novel therapies for asthma: Advances and problems. Curr Pharmaceut Design. 2005; 11: 3027-38.

Wills-Karp M. Immunologic basis of antigen-induced airway hyperresponsiveness. Annu Rev Immunol. 1999; 17: 255-81.

Wu X, Beecher GR, Holden J, Haytowitz DB, Gebhardt SE, Prior RL. Lipophylic and hidrophylic antioxidant capacities of common foods in United States. J Agric Food Chem. 2004, 52: 4026-37.

Published
2015-02-14
How to Cite
Dai, Y.-L., G.-L. Shao, and F. Wang. “Extract of Brassica Rapa Suppresses Ovalbumin-Induced Asthma by Down-Regulating NF-KappaB and P38 MAPK Activity”. Bangladesh Journal of Pharmacology, Vol. 10, no. 1, Feb. 2015, pp. 106-14, doi:10.3329/bjp.v10i1.21214.
Section
Research Articles