Scopoletin potentiates the anti-cancer effects of cisplatin against cholangiocarcinoma cell lines

  • Md. Ali Asgar Program in Biological Science, Khon Kaen University, Khon Kaen 40002
  • Gulsiri Senawong Department of Biochemistry, Faculty of Science, Khon Kaen University, Khon Kaen 40002
  • Banchob Sripa Department of Pathology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002
  • Thanaset Senawong Program in Biological Science, Khon Kaen University, Khon Kaen 40002
Keywords: Cholangiocarcinoma, Cisplatin, Drug combination, Scopoletin

Abstract

Chemotherapy with cisplatin in cholangiocarcinoma produces adverse effects and leads to resistance development by tumors. We aimed to evaluate anti-cancer effects by co-administration of cisplatin and scopoletin in cholangiocarcinoma cells. MTT assay, median effect principle, cell cycle arrest and apoptosis assay were conducted to determine anti-cancer effects. Results revealed that treatment with cisplatin and scopoletin resulted in dose-dependent reduction of cell viability for cholangiocarcinoma cells. Combination of these agents inhibited proliferation of cells significantly more than single agent either. Combination indices reflect additive cytotoxic effect, leading to >2 times dose reduction for each agent. Both the cell cycle arrest (G0/G1) and apoptosis induction underling the enhanced cytotoxicity for the combination. Besides, single agent conferred cell cycle arresting and apoptotic effects in cholangiocarcinoma cells. By contrast, non-cancer cells were less affected with combination. Our observations suggest that cisplatin and scopoletin combination may bring positive significance in cholangiocarcinoma treatment.

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Published
2015-01-21
How to Cite
Asgar, M., G. Senawong, B. Sripa, and T. Senawong. “Scopoletin Potentiates the Anti-Cancer Effects of Cisplatin Against Cholangiocarcinoma Cell Lines”. Bangladesh Journal of Pharmacology, Vol. 10, no. 1, Jan. 2015, pp. 69-77, doi:10.3329/bjp.v10i1.21202.
Section
Research Articles