Nobiletin acts as a potential anticancer agent against osteosarcoma by regulating ERK and AKT signaling pathways

  • Fu-Wen Niu Department of Orthopedics, Jining No.1 People’s Hospital in Shandong Province
  • Yong-Jie Zhang Department of Orthopedics, JinXiang People’s Hospital in Shandong Province
  • Kun Li Department of Gynecology and Obstetrics, Affiliated Hospital of Shandong Academy of Medical Sciences
  • Mao-Shu Zhang Department of Orthopedics, Jining No.1 People’s Hospital in Shandong Province
Keywords: Antiproliferative, Nobiletin, Osteosarcoma, Signaling pathway


The current study was aimed to evaluate the anti-proliferative activity of nobiletin, a flavonoid, on osteosarcoma cell line, SaOS-2. Furthermore, we attempted to investigate the signaling pathways involved in the cell growth inhibition mechanism. Upon exposure, nobiletin showed significant cell inhibitory effect on SaOS-2 cells in a dose dependent manner as evidenced by MTT assay and LDH assay. Moreover, flow cytometric analysis demonstrated that upon treatment with nobiletin, SaOS-2 cells underwent apoptosis and showed increased number of cells arrested in G2/M phase. Activation of caspase-9 and -3 indicate the activation of apoptotic signaling pathway in SaOS-2 cells. Additionally, it was observed that the cell inhibitory effect was regulated by the activation of ERK signaling pathway while AKT signaling pathway was down regulated in SaOS-2 cells after 24 h of Nobiletin treatment.


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How to Cite
Niu, F.-W., Y.-J. Zhang, K. Li, and M.-S. Zhang. “Nobiletin Acts As a Potential Anticancer Agent Against Osteosarcoma by Regulating ERK and AKT Signaling Pathways”. Bangladesh Journal of Pharmacology, Vol. 9, no. 3, Sept. 2014, pp. 406-12, doi:10.3329/bjp.v9i3.19567.
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