Hepatoprotective activity of methanolic extract of Malva parviflora against paracetamol-induced hepatotoxicity in mice

  • Tauqeer Hussain Mallhi School of Pharmaceutical Sciences, University Sains Malaysia, Palau Penang
  • Khizar Abbas College of Pharmacy, Government College University Faisalabad
  • Muhammad Ali Institute of Molecular Biology & Biotechnology, Bahauddin Zakariya University, Multan
  • Muhammad Imran Qadir Institute of Molecular Biology & Biotechnology, Bahauddin Zakariya University, Multan
  • Mohammad Saleem College of Pharmacy, Government College University Faisalabad
  • Yusra Habib Khan School of Pharmaceutical Sciences, University Sains Malaysia, Palau Penang
Keywords: Hepatoprotective, Malva parviflora, Paracetamol

Abstract

Malva parviflora (cheeseweed) is traditionally used as hepatoprotective. The current study was conducted to determine its hepatoprotective activity of aqueous methanolic extract of whole plant. Two doses of plant (250 mg/kg and 500 mg/kg) were administered in paracetamol intoxicated mice and results were compared with silymarin. Observational parameters were ALT, AST, ALP and total bilirubin. The results showed that the extract of M. parviflora produced significant (p<0.001) reduction in liver enzymes and total bilirubin. Results were supported by histopathological investigation, phytochemical screening and detection of hepatoprotective constituents (kaempferol and apigenin) by HPLC. So, the current study showed that aqueous methanolic extract of M. parviflora possesses hepatoprotective activity.

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Author Biography

Muhammad Imran Qadir, Institute of Molecular Biology & Biotechnology, Bahauddin Zakariya University, Multan
Assistant Professor

References

Afolayan AJ, Aboyade OM, Adedapo AA, Sofidiya MO. Anti-inflammatory and analgesic activity of the methanol extract of Malva parviflora Linn (Malvaceae) in rats. Afr J Biotech. 2010; 9: 1225-29.

Ahmad M, Mahmood Q, Gulzar K, Akhtar MS, Saleem M, Qadir MI. Antihyperlipidaemic and hepatoprotective activity of Dodonaea viscosa leaves extracts in alloxan-induced diabetic rabbits (Oryctolagus cuniculus). Paki Vet J. 2012; 32: 50-54.

Akhtar MS, Asjad HMM, Bashir S, Malik A, Khalid R, Gulzar F, Irshad N. Evaluation of antioxidant and Hepatoprotective effects of Khamira Gaozaban Ambri Jadwar Ood Saleeb Wala (KGA). Bangladesh J Pharmacol. 2013; 8: 44-48.

Ali M, Qadir MI, Saleem M, Janbaz KH, Gul H, Hussain L, Ahmed B. Hepatoprotective potential of Convolvulus arvensis against paracetamol-induced hepatotoxicity. Bangladesh J Pharmacol. 2013; 8: 300-04.

Amin N, Qadir MI, Khan TJ, Abbas G, Ahmad B, Janbaz KH, Ali M. Antibacterial activity of vacuum liquid chromatography (VLC) isolated fractions of chloroform extracts of seeds of Achyranthes aspera. J Chem Soc Pak. 2012; 34: 589-92.

Amiri MS, Joharchi MR, TaghavizadehYazdi ME. Ethno-medicinal plants used to cure jaundice by traditional healers of Mashhad, Iran. Iranian J Pharm Res. 2014; (Ahead of print)

Arora DS, Kaur GJ. Antibacterial activity of some Indian medicinal plants. Natl Med J. 2007; 61: 313-17.

Asif MA, Qadir MI (2011) Molecular approaches for development of malarial vaccines. Rev Pharmacol. 2011; 4: 276-78.

Bag AK, Mumtaz SMF. Hepatoprotective and nephroprotec-tive activity of hydroalcoholic extract OfIpomoea staphylina leaves. Bangladesh J Pharmacol. 2013; 8: 263-68.

Balamurugan G, Muthusamy P. Observation of the Hepatoprotective and antioxidant activities of Trianthema decandra Linn. (Vallai sharunnai) roots on carbon tetrachloridetreated rats. Bangladesh J Pharmacol. 2008; 3: 83-89.

Chattopadhyay RR. Possible mechanism of hepatoprotective activity of Azadirachta indica leaf extract. J Ethnopharmacol. 2003; 89: 217-19.

Ehsan O, Qadir MI, Malik SA, Abbassi WS, Ahmad B. Efficacy of nanogold-insulin as a hypoglycemic agent. J Chem Soc Pak. 2012; 34: 365-70.

Farhan H, Rammal H, Hijazi A, Badran B. Preliminary phytochemical screening and extraction of polyphenol from stems and leaves of a Lebanese plant Malva parviflora L. Int J Curr Pharma Res. 2012; 4: 55-59.

Gutierrez RMP. Evaluation of hypoglycemic activity of the leaves of Malva parviflora in streptozotocin-induced diabetic rats. Food Func. 2012; 3: 420-27.

Hegde K, Joshi AB. Hepatoprotective and antioxidant effect of Carissa spinarum root extract against CCl4 and paracetamol-induced hepatic damage in rats. Bangladesh J Pharmacol. 2010; 5: 73-76.

Hussain A, Khalid SH, Qadir MI, Massud A, Ali M, Khan IU, Saleem M, Iqbal MS, Asghar S, Gul H. Water Uptake and Drug Release Behaviour of Methyl Methacrylate-co-itaconic acid [P(MMA/IA)] Hydrogels Cross-linked with Methylene Bis-acrylamide. J Drug Deliv Sci Tech. 2011; 21: 249-55.

Janbaz KH, Nisar U, Ashraf M, Qadir MI. Spasmolytic, bronchodilator and antioxidant activities of Erythrina superosa Roxb. Acta Pol Pharm. 2012; 69: 1111-17.

Janbaz KH, Saeed SA, Gilani, AH. Studies on the protective effects of caffeic acid and quercetin on chemical-induced hepatotoxicity in rodents. Phytomedicine 2004; 11: 42430.

Javed F, Qadir MI, Janbaz KH, Ali M. Novel drugs from marine microorganisms. Cr Rev Microbiol. 2011; 37: 245-49.

Khalid SH, Qadir MI, Massud A, Ali M, Rasool MH. Effect of degree of cross-linking on swelling and drug release behaviour of poly (methyl methacrylate-co-itaconic acid) [P(MMA/IA)] hydrogels for site specific drug delivery. J Drug Deliv Sci Tech. 2009; 19: 413-18.

Khan AZ, Mohammad A, Iqbal Z, Anis I, Shah MR, Nadeem S, Rabnawaz M, Shahidullah A, Khan H, Khan I. Molecular docking of viscosine as a new lipoxygenase inhibitor isolated from Dodonaea viscose. Bangladesh J Pharmacol. 2013; 8: 36-39.

Khattak SG, Gilani SN, Ikram M. Antipyretic studies on some indigenous Pakistani medicinal plants. J Ethnopharmacol. 1985; 14: 45-51.

Koochek M, Pipelzadeh M, Mardani H. The effectiveness of Viola odorata in the prevention and treatment of formalin induced lung damage in the rat. J Herbs Spices Med Plants. 2003; 10: 95-103.

Masood MI, Qadir MI, Shirazi JH, Khan IU. Beneficial effects of lactic acid bacteria on human beings. Crit Rev Microbiol. 2011; 37: 9198.

Naz S, Qadir MI, Ali M, Janbaz KH. Nanotechnology for imaging and drug delivery in cancer. J Chem Soc Pak. 2011; 34: 107-111.

Oh H, Kim DH, Cho JH, Kim YC. Hepatoprotective and free radical scavenging activities of phenolic petrosins and flavonoids isolated from Equisetum arvense. J Ethnopharmacol. 2004; 95: 421-24.

Qadir MI, Ali M, Ali M, Saleem M, Hanif M. Hepatoprotective activity of aqueous methanolic extract of Viola odorata against paracetamol-induced liver injury in mice. Bangladesh J Pharmacol. 2014b; 9: 198-202.

Qadir MI, Malik SA. Anti-diabetic activity of inorganic metals Eugenia jambolana Lam. (Myrtaceae) flowers. Pharmacologyonline 2010; 2: 979-85.

Qadir MI, Malik SA. Effect of Eugenia jambolana leaves extracts on blood glucose levels of experimental diabetic rabbits. Pharmacologyonline 2009; 3: 829-35.

Qadir MI, Murad MSA, Ali M, Saleem M, Farooqi AA. Hepatoprotective effect of leaves of aqueous ethanol extract of Cestrum nocturnum against paracetamol-induced hepatotoxicity. Bangladesh J Pharmacol. 2014a; 9: 167-70.

Qadir MI. Medicinal and cosmetological importance of Aloe vera. Int J Nat Ther. 2009; 2: 21-26.

Qadir MI. Medicinal values of ginger. Int J Nat Ther. 2009; 3: 19-22.

Qadir MI. Qadirvirtide. Pak J Pharm Sci. 2011; 24: 593-95.

Saleem M, Ahmed B, Qadir MI, Mahrukh, Rafiq M, Ahmad M, Ahmad B. Hepatoprotective effect of Chenopodium murale in mice. Bangladesh J Pharmacol. 2014; 9: 124-28.

Shale TL, Stirk WA, Van Staden J. Variation in antibacterial and anti-inflammatory activity of different growth forms of Malva parviflora and evidence for synergism of the anti-inflammatory compounds. J Ethnopharmacol. 2005; 96: 325-30.

Song EK, Kim JH, Kim JS, Cho H, Nan JX, Sohn DH, Ko GI, Oh H, Kim YC. Hepatoprotective phenolic constituents of Rhodiola sachalinensis on tacrine?induced cytotoxicity in Hep G2 cells. Phytother Res. 2003; 17: 563-65.

Wang X, Bunkers GJ, Walters MR, Thoma RS. Purification and characterization of three antifungal proteins from cheeseweed (Malva parviflora). Biochem Biophys Res Commun. 2001; 282: 1224-28.

Published
2014-08-02
How to Cite
Mallhi, T., K. Abbas, M. Ali, M. Qadir, M. Saleem, and Y. Khan. “Hepatoprotective Activity of Methanolic Extract of Malva Parviflora Against Paracetamol-Induced Hepatotoxicity in Mice”. Bangladesh Journal of Pharmacology, Vol. 9, no. 3, Aug. 2014, pp. 342-6, doi:10.3329/bjp.v9i3.19105.
Section
Research Articles