Hepatoprotective effect of aqeous methanolic extract of Rumex dentatus in paracetamol induced hepatotoxicity in mice

  • Mohammad Saleem College of Pharmacy, GC University Faisalabad, Pakistan
  • Bilal Ahmed College of Pharmacy, GC university Faisalabad
  • Mahrukh Karim College of Pharmacy, GC University Faisalabad
  • Shahzad Ahmed Draxel University College of Medicines, Philadelphia, USA
  • Mobasher Ahmad University College of Pharmacy, University of The Punjab Lahore
  • M. Imran Qadir Institute of Molecular Biology & Biotechnology, Bahauddin Zakariya University, Multan
  • Nawazish-i-Husain Syed University College of Pharmacy, University of The Punjab Lahore, Pakistan
Keywords: Hepatoprotective, HPLC, Paracetamol, Rumex dentatus


Rumex dentatus is well known plant of Polygonaceae family which is widely used in traditional medicine to treat gastrointestinal tract. R. dentatus at doses 250 and 500 mg/kg significance decreased (p<0.001) the elevated level of ALT, AST, ALP and bilirubin induced by paracetamol and results are comparable (p<0.001) with silymarin. The results were supported by histopathological investigations, phytochemical screening and detection of hepatoprotective active constituents e.g quercetin, kaempferol, myricetin by HPLC. So, it is concluded that R. dentatus has hepatoprotective effect against paracetamol liver damage in mice.


Download data is not yet available.
1100 Read


Ali M, Qadir MI, Saleem M, Janbaz KH, Gul H, Hussain L, Ahmad B. Hepatoprotective potential of Convolvulus arvensis against paracetamol-induced hepatotoxicity. Bangladesh J Pharmacol. 2013; 8: 300-04.

Akhtar MS, Asjad HMM, Bashir S, Malik A, Khalid R, Gulzar F, Irshad N. Evaluation of anti-oxidant and hepatoprotective effects of Khamira Gaozaban Ambri Jadwar Ood Saleeb Wala (KGA). Bangladesh J Pharmacol. 2013; 8: 44-48.

Arosio B, Gagliano N, Fusaro LMP, Parmeggiani L, Tagliabue J, Galetti P, Annoni G. Aloe‐emodin quinone pretreatment reduces acute liver injury induced by carbon tetrachloride. Pharmacol Toxicol. 2008; 87: 229-33.

Bag AK, Mumtaz SMF. Hepatoprotective and nephroprotective activity of hydroalcoholic extract OfIpomoea staphylina leaves. Bangladesh J Pharmacol. 2013; 8: 263-68.

Bate‐Smith E. The phenolic constituents of plants and their taxonomic significance. I. Dicotyledons. J Linn Soc Bot. 1962; 58: 95-173.

Elzaawely AA, Tawata S. Anti-oxidant capacity and phenolic content of Rumex dentatus L. Grown in Egypt. J Crop Sci Biotech. 2012; 15: 59-64.

Fatima N, Zia M, Rehman R, Rizvi ZF, Ahmad S, Mirza B, Chaudhary MF. Biological activities of Rumex dentatus L: Evaluation of methanol and hexane extracts. Afr J Biotechnol. 2009; 8: 6945-51.

Gnanadesigan M, Ravikumar S, Inbaneson SJ. Hepatoprotective and anti-oxidant properties of marine halophyte Luminetzera racemosa bark extract in CCL4-induced hepatotoxicity. Asian Pac J Trop Med. 2011; 4: 462-65

Girish C, Koner BC, Jayanthi S, Ramachandra RK, Rajesh B, Pradhan SC. Hepatoprotective activity of picroliv, curcumin and ellagic acid compared to silymarin on paracetamol induced liver toxicity in mice. Fundam Clin Pharmacol. 2009; 23: 735-45.

Gujrati V, Patel N, Rao VN, Nandakumar K, Gouda T. Hepatoprotective activity of alcoholic and aqueous extracts of leaves of Tylophora indica (Linn.) in rats. Indian J Pharmacol. 2007; 39: 43.

Hameed I, Dastagir G. Nutritional analyses of Rumex hastatus D. Don, Rumex dentatus Linn and Rumex nepalensis Spreng. Afr J Biotechnol. 2009; 8: 4131-33.

Heibatollah S, Mohammad Reza N, Izadpanah G and Sohailla S. Hepatoprotective effect of Cichorium intybus on CCl4-induced liver damage in rats. Afr J Biochem Res. 2008; 2: 141-44

Hussain F, Ahmad B, Hameed I, Dastagir G, Sanaullah P, Azam S. Antibacterial, antifungal and insecticidal activities of some selected medicinal plants of polygonaceae. Afr J Biotechnol. 2010; 9: 5032-36.

Itoh A, Isoda K, Kondoh M, Kawase M, Kobayashi M, Tamesada M, Yagi K. Hepatoprotective effect of syringic acid and vanillic acid on concanavalin A-induced liver injury. Biol Pharm Bull. 2009; 32: 1215-19.

Janbaz K, Saeed S, Gilani A. Studies on the protective effects of caffeic acid and quercetin on chemical-induced hepatotoxicity in rodents. Phytomedicine. 2004; 11: 424-30.

Kumar B, Lakshman K, Swamy V, Kumar P, Shekar D, Manoj B, Vishwantha G. Hepatoprotective and anti-oxidant activities of Amaranthus viridis Linn. Macedonian J Med Sci. 2011; 4: 125-30.

Litvinenko YA, Muzychkina R. Phytochemical investigation of biologically active substances in certain Kazakhstan Rumex species. Chem Nat Compd. 2003; 39: 446-49.

Makni M, Chtourou Y, Fetoui H, Garoui EM, Boudawara T, Zeghal N. Evaluation of the anti-oxidant, anti-inflammatory and hepatoprotective properties of vanillin in carbon tetrachloride-treated rats. Eur J Pharmacol. 2011; 668: 133-39.

Matić S, Stanić S, Bogojević D, Vidaković M, Grdović N, Dinić S, Mihailović M. Methanol extract from the stem of Cotinus coggygria Scop., and its major bioactive phytochemical constituent myricetin modulate pyrogallol-induced DNA damage and liver injury. Mutat Res-Gen Tox En. 2013; 755: 81-89.

Manandhar N. Plants and people of Nepal timber press. Oregon 2002; 192: 527-26.

Mojab F, Kamalinejad M, Ghaderi N, Vahidipour HR. Phytochemical screening of some species of Iranian plants. IJPR. 2003; 4: 77-82.

Raju K, Anbuganapathi G, Gokulakrishnan V, Rajkapoor B, Jayakar B, and Manian M. Effect of dried fruits of Solanum nigrum Linn against CCl4-induced hepatic damage in rats. Biol Pharm Bull. 2003; 26: 1618-19.

Setty SR, Quereshi AA, Swamy AHMV, Patil T, Prakash T, Prabhu K, Gouda AV. Hepatoprotective activity of Calotropis procera flowers against paracetamol-induced hepatic injury in rats. Fitoterapia 2007; 78: 451-54.

Rashed KN, Chang CW, Wu LY, Peng WH. Hepatoprotective activity of Diospyros lotus fruits on acute liver injury induced by carbon tetrachloride and phytochemical analysis. Topcls J Herb Med. 2013; 2: 75-83.

Ravikumar S, Gnanadesigan M. Hepatoprotective and anti-oxidant Properties of Rhizophora mucronata plant in CCl4 Intoxicated Rats. J Exp Clin Med. 2012; 4: 66-72.

Rehman JU, Saqib NU, Akhtar N, Jamshaid M, Asif HM, Sultana S, Rehman RU. Hepatoprotective activity of aqueous-methanolic extract of Suaeda fruticosa in paracetamol-induced hepatotoxicity in rabbits. Bangladesh J Pharmacol. 2013; 8: 378-81.

Sabir S, Rocha J. Water-extractable phytochemicals from Phyllanthus niruri exhibit distinct in vitro anti-oxidant and in vivo hepatoprotective activity against paracetamol-induced liver damage in mice. Food Chem. 2008; 111: 845-51.

Saboo SS, Tapadiya G, Farooqui IA, Khadabadi SS. Free radical scavenging, in vivo anti-oxidant and hepatoprotective activity of folk medicine Trichodesma sedgwickianum. Bangladesh J Pharmacol. 2013; 8: 58-64.

Sahreen S, Khan MR, Khan RA. Hepatoprotective effects of methanol extract of Carissa opaca leaves on CCl4-induced damage in rat. BMC Complem Altern Med. 2011; 11: 48.

Saleem M, Ahmed B, Qadir MI, Karim M, Rafiq M, Ahmad M, Ahmad B. Hepatoprotective effect of Chenopodium murale in mice. Bangladesh J Pharmacol. 2014; 9: 124-28.

Sathaye S, Bagul Y, Gupta S, Kaur H, Redkar R. Hepatoprotective effects of aqueous leaf extract and crude isolates of Murraya koenigii against in vitro ethanol-induced hepatotoxicity model. Exp Toxicol Pathol. 2011; 63: 587-91.

Samuel AJSJ, Mohan S, Chellappan DK, Kalusalingam A, Ariamuthu S. Hibiscus vitifolius (Linn.) root extracts shows potent protective action against anti-tubercular drug induced hepatotoxicity. J Ethnopharmacol. 2012; 141: 396-402.

Sharma PP, Roy RK, Anurag GD. Pentacyclic triterpinoids from Betula utilis and Hyptis suaveolens. IJPTR. 2010; 1558-32.

Simonyan A. Activity of cinnamic acid derivatives and new methods for their synthesis. Pharm Chem J. 1993; 27: 92-100.

Sofowora. Medicinal plants and traditional medicine in Africa. New York, John Wiley and Sons Ltd, 1993.

Song LR, Hu L. Rumex dentatus. Traditional Chinese herb. 1st ed. Shanghai, Science and Technology Publisher, 1999, p 727.

Song EK, Kim JH, Kim JS, Cho H, Nan JX, Sohn DH, Ko Gl, Oh H, Kim YC. Hepatoprotective phenolic constituents of Rhodiola sachalinensis on tacrine‐induced cytotoxicity in Hep G2 cells. Phytother Res. 2003; 17: 563-65.

Srinivasan M, Sudheer AR, Menon VP. Ferulic acid: Therapeutic potential through its anti-oxidant property. J Clin Biochem Nutr. 2007; 40: 92.

Sultana B, Anwar F, Rafique AM, Chatha SAH. Anti-oxidant potential of extracts from different agro wastes: Stabilization of corn oil. Grasasy Aceites, 2008; 59: 205-17.

Süleyman H, Demirezer L Ö, Kuruüzüm A, Banoğlu Z, Göçer F, Özbakir G, Gepdiremen A. Anti-inflammatory effect of the aqueous extract from Rumex patientia L roots. J Ethnopharmacol. 1999; 65: 141-48.

Umer A, Yousaf Z, Khan F, Hussain U, Anjum A, Nayyab Q, Younas A. Evaluation of allelopathic potential of some selected medicinal species. Afr J Biotechnol. 2010; 9: 6194-206.

Vijay N, Padmaa M. Hepatoprotective activity of Chenopodium album Linn. against paracetamol induced liver damage. Pharmacologyonline 2011; 3: 312-28.

Vijayan P, Prashanth H, Vijayaraj P, Dhanaraj S, Badami S, Suresh, B. Hepatoprotective effect of the total alkaloid fraction of Solanum pseudocapsicum leaves. Pharma Biol. 2003; 41: 443-48.

Zhang H, Guo Z, Wu N, Xu W, Han L, Li N, Han Y. Two novel naphthalene glucosides and an anthraquinone isolated from Rumex dentatus and yheir antiproliferation activities in four cell lines. Molecules 2012; 17: 843-50.

How to Cite
Saleem, M., B. Ahmed, M. Karim, S. Ahmed, M. Ahmad, M. I. Qadir, and N.- i-H. Syed. “Hepatoprotective Effect of Aqeous Methanolic Extract of Rumex Dentatus in Paracetamol Induced Hepatotoxicity in Mice”. Bangladesh Journal of Pharmacology, Vol. 9, no. 3, July 2014, pp. 284-9, doi:10.3329/bjp.v9i3.18874.
Research Articles