2,4,3',4'-tetramethoxy-biphenyl induces apoptosis in MGC-803 cells through a mitochondrial/caspase pathway

Authors

  • Xin Sui School of Chemistry and Materials Science, Heilongjiang University, Harbin 150080
  • Zhizhong Sun School of Chemistry and Materials Science, Heilongjiang University, Harbin 150080
  • Weiwei An Institute of Tumor Research, Harbin Medical University, Harbin 150081
  • Kai Cheng School of Chemistry and Materials Science, Heilongjiang University, Harbin 150080
  • Shougang Jiang Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040
  • Gong Xianfeng School of Chemistry and Materials Science, Heilongjiang University, Harbin 150080

DOI:

https://doi.org/10.3329/bjp.v9i2.18430

Keywords:

Apoptosis, Caspases, MGC-803 cells, Mitochondria, Tetramethoxybiphenyl

Abstract

Anti-proliferative and apoptosis-inducing effects of 2,4,3?,4?-tetramethoxy-biphenyl (TMBP) on human gastric cancer MGC-803 cells were investigated. The molecular mechanisms of TMBP-mediated tumor cell death were detected by clonogenic assay, staining with Hoechst 33258, DNA fragmentation assay, Western blot analysis and flow cytometry assay. Studies on MGC-803 cells treated with TMBP showed that TMBP inhibited the proliferation of MGC-803 cells in a time- and dose-dependent manner. The induction of apoptosis by TMBP was accompanied by the loss of mitochondrial membrane potential (ΔΨm), cytochrome C release and activation of caspase cascade, resulting in the cleavage of some specific substrates for caspase-3 such as poly (ADP-ribose) polymerase (PARP). In conclusion, these findings showed that TMBP may induce the apoptosis of MGC-803 through a mitochondrial/caspase pathway, suggesting its possible use for treating human cancers.

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Published

2014-06-02

How to Cite

Sui, X., Z. Sun, W. An, K. Cheng, S. Jiang, and G. Xianfeng. “2,4,3’,4’-Tetramethoxy-Biphenyl Induces Apoptosis in MGC-803 Cells through a mitochondrial/Caspase Pathway”. Bangladesh Journal of Pharmacology, vol. 9, no. 2, June 2014, pp. 235-43, doi:10.3329/bjp.v9i2.18430.

Issue

Section

Research Articles