In vitro antimicrobial screening of methanolic extracts of Cleome chelidonii and Cleome gynandra

Authors

  • Nimmakayala Sridhar Department of Pharmacology, Pydah College of Pharmacy, Kakinada, A.P
  • Bondada V.V.S. Surya Kiran Department of Pharmaceutical technology, Koringa College of Pharmacy, Korangi, A.P
  • Donthamsetti Tharaka Sasidhar Department of Pharmacology, Pydah College of Pharmacy, Kakinada, A.P
  • Lakshmi Kanta Kanthal Department of Pharmacology, Koringa College of Pharmacy, Korangi, A.P

DOI:

https://doi.org/10.3329/bjp.v9i2.17759

Keywords:

Cleome chelidonii, Cleome gynandra, Minimum inhibitory concentration

Abstract

Antimicrobial activity of methanolic extracts of plants was screened by disc diffusion assay against four bacteria and four fungal cultures. Streptomycin (10 ?g/disc) and nystatin (10 ?g/disc) are used as standards for bacteria and fungi respectively. Minimum inhibitory concentration (MIC) of the extracts was evaluated through micro broth dilution method. The antimicrobial potency of plant extracts was assessed by their zone of inhibition and activity index values. Total activity of extracts was evaluated to quantitatively compare the activity of two plants. Methanolic extract of Cleome gynandra showed maximum antibacterial activity against Staphylococcus aureus (IZ- 22 ± 0.22 mm, AI-0.917, MIC- 0.039 mg/mL, MBC- 0.039 mg/mL). Maximum antifungal potential was shown by C. chelidonii against Candida albicans (IZ- 25 ± 0.92 mm, AI-1.000, MIC- 0.039 mg/mL, MFC- 0.039 mg/mL). Both the extracts exhibited good antimicrobial activity with low range of MIC.

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Additional Files

Published

2014-03-30

How to Cite

Sridhar, N., B. V. Surya Kiran, D. T. Sasidhar, and L. K. Kanthal. “In Vitro Antimicrobial Screening of Methanolic Extracts of Cleome Chelidonii and Cleome Gynandra”. Bangladesh Journal of Pharmacology, vol. 9, no. 2, Mar. 2014, pp. 161-6, doi:10.3329/bjp.v9i2.17759.

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Research Articles