In vitro antimicrobial screening of methanolic extracts of Cleome chelidonii and Cleome gynandra


  • Nimmakayala Sridhar Department of Pharmacology, Pydah College of Pharmacy, Kakinada, A.P
  • Bondada V.V.S. Surya Kiran Department of Pharmaceutical technology, Koringa College of Pharmacy, Korangi, A.P
  • Donthamsetti Tharaka Sasidhar Department of Pharmacology, Pydah College of Pharmacy, Kakinada, A.P
  • Lakshmi Kanta Kanthal Department of Pharmacology, Koringa College of Pharmacy, Korangi, A.P



Cleome chelidonii, Cleome gynandra, Minimum inhibitory concentration


Antimicrobial activity of methanolic extracts of plants was screened by disc diffusion assay against four bacteria and four fungal cultures. Streptomycin (10 ?g/disc) and nystatin (10 ?g/disc) are used as standards for bacteria and fungi respectively. Minimum inhibitory concentration (MIC) of the extracts was evaluated through micro broth dilution method. The antimicrobial potency of plant extracts was assessed by their zone of inhibition and activity index values. Total activity of extracts was evaluated to quantitatively compare the activity of two plants. Methanolic extract of Cleome gynandra showed maximum antibacterial activity against Staphylococcus aureus (IZ- 22 ± 0.22 mm, AI-0.917, MIC- 0.039 mg/mL, MBC- 0.039 mg/mL). Maximum antifungal potential was shown by C. chelidonii against Candida albicans (IZ- 25 ± 0.92 mm, AI-1.000, MIC- 0.039 mg/mL, MFC- 0.039 mg/mL). Both the extracts exhibited good antimicrobial activity with low range of MIC.


Download data is not yet available.
1734 Read


Alamzeb M, Khan MR, Ali S, Shah SQ, Rashid MU. Antimicrobial properties of extracts and compounds isolated from Berberis jaeschkeana. Bangladesh J Pharmacol. 2013; 8: 107-09.

Andrews JM, BSAC Working party on susceptibility testing ft. BASC standardized disc susceptibility testing method. J Antimicrob Chemother. 2001; 4: 43-57.

Baker CN, Thornsberg CH. Inoculum standardization in antimicrobial susceptibility tests: Evaluation of overnight age culture. J Chin Microbiol. 1983; 17:140-57.

Bakar RA, Ahmad I, Sulaiman SF. Effect of Pithecellobium jiringa as antimicrobial agent. Bangladesh J Pharmacol. 2012; 7: 131-34.

Barchiesi F, Spreghini E, Tomassetti S, Della AV, Arzeni D, Manso E, Scalise G. Effects of Caspo fungin against Candida guilliermondii and Candida parapsilosis. Antimicrob Agents Chemother. 2006; 50: 2719-27.

Barsi DF, Fan SH. The potential of aqueous and acetone extracts of galls of Quercus infectoria as antibacterial agents. Indian J Pharmacol. 2005; 37: 26-29.

Bouguerra R, Essaïs O, Sebaï N, Ben Salem L, Amari H, Kammoun MR, Chaker E, Zidi B, Ben Slama C. 2004. Prévalence et aspects cliniques des mycoses superficielles chez le diabétique tunisien en milieu hospitalier. Flavour Fragrance J. 2004; 18: 210-15.

Das PC, Patra A, Mandal S, Mallick B, Das A, Chatterjee A. Cleogynol, a novel dammarane triterpenoid from Cleome gynandra. J Nat Prod. 1999; 62: 616-18.

Das SN, Patro VJ, Dinda SC. Antimicrobial activity of Leucas clarkei. Bangladesh J Pharmacol. 2012; 7: 135-39.

Eloff JN. Quantifying the bioactivity of the plant extracts during screening and bioassay guided fractionation. Phytomedicine 2004; 11: 370-71.

Ethadi S, Pragada R, Battu G. Evolution of anti-inflammatory and hepatoprotective activities of different extracts of Cleome chelidonii root in albino rats. Int J Pharma Biosci. 2013; 4: 111-19.

Fawzy GA, Gamal AAE, Ati HYA. Antimicrobial and cytotoxic potentials of Buddleja polystachya extracts. Bangladesh J Pharmacol. 2013; 8: 136-141.

Gupta RK, Chandra S, Mahadevan V. Chemical examination of the seed of Gynandropsis petaphylla. Indian J Pharm. 1968; 30: 127-28.

Iqbal SM, Mushtaq A, Jabeen Q. Antimicrobial, antioxidant and calcium channel blocking activities of Amberboa divaricata. Bangladesh J Pharmacol. 2014; 9: 29-36.

Jain AC, Gupta SM. Minor phenolic components of the seeds of Gynandropsis gynandra. J Nat Prod. 1985; 48: 332-33.

Kirtikar KR, Basu BD. In: Indian medicinal plants, Vol. 1. 2nd ed. Delhi, Periodical Experts Book Agency, 1991.

Mazumder AH, Das J, Gogoi HK, Chattopadhyay P, Paul SB. Antimicrobial activity of methanol extract and fractions from Sarcochlamys pulcherrima. Bangladesh J Pharmacol. 2014; 9: 4-9.

Mule SN, Patil SB, Naikwade NS, Magdum CS. Evaluation of antinociceptive and anti-inflammatory activity of stems of Gynandropsis pentaphylla Linn. Int J Green Pharm. 2008; 2: 87-90.

Murray PR, Baron EJ, Pfaller MA, Tenover FC, Yolke RH. Manual of clinical microbiology. 6th ed. London, Mosby Year Book, 1995.

Nisar M, Ali S, Qaisar M, Gilani SN, Shah MR, Khan I, Ali G. Antifungal activity of bioactive constituents and bark extracts of Rhododendron arboretum. Bangladesh J Pharmacol. 2013; 8: 218-22.

Ntsoelinyane PMAH, Mashele S. Phytochemical screening, antibacterial and antioxidant activities of Asparagus laricinus leaf and stem extracts. Bangladesh J Pharmacol. 2014; 9: 10-14.

Parimalakrishnan S, Dey A, Smith A, Manavalan R. Evaluation of anti-inflammatory, antinociceptive and antipyretic effects of methanol extract of Cleome chelidonii. Int J Biol Chem Sci. 2007; 1: 223-28.

Rastogi B, Tiwari U, Dubey A, Bawra B, Nandini D, Chauhan NS, Saraf DK. Immunomodulating activity of Cleome gynandra. Pharmacologyonline 2009; 2: 151-69.

Shah MA, Abdullah SM, Khan MA, Nasar G, Saba I. Antibacterial activity of chemical constituents isolated from Asparagus racemosus. Bangladesh J Pharmacol. 2014; 9: 1-3.

Shaik K, Shaik A, Kumar D, Kadirvel D. Evaluation of preliminary phytochemical properties and hypoglycemic activities of Cleome gynandra L. Int J Pharm Pharmaceut Sci. 2013; 5: 824-28.

Shen X, Zheng D, Gao J, Hou C. Isolation and evaluation of endophytic fungi with antimicrobial ability from Phyllostachys edulis. Bangladesh J Pharmacol. 2012; 7: 249-57.

Singh G, Kumar P. Phytochemical study and screening for antimicrobial activity of flavonoids of Euphorbia hirta. Int J Appl Basic Med Res. 2013; 3: 111-16.

Songsak T, Lockwood GB. Production of two volatile glucose-nolate hydrolysis compounds in Nasturtium montanum and Cleome chelidonii plant cell cultures. Fitoterapia 204; 75: 296-301.

The Ayurvedic Pharmacopoeia of India. Part 1. Vol 1. 2007, p 13.

Sridhar N, Kumar NVSP, Sasidhar D, Venkatesh AK, Kanthal LK. Antibactirial and phytochemical evaluation of Oldenlandia biflora L. and Pergularia daemia. Int J Drug Dev Res. 2012; 4: 148-52.

Zhang H, Bai X, Wu B. Evaluation of antimicrobial activities of extracts of endophytic fungi from Artemisia annua. Bangladesh J Pharmacol. 2012; 7: 120-23.

Additional Files



How to Cite

Sridhar, N., B. V. Surya Kiran, D. T. Sasidhar, and L. K. Kanthal. “In Vitro Antimicrobial Screening of Methanolic Extracts of Cleome Chelidonii and Cleome Gynandra”. Bangladesh Journal of Pharmacology, vol. 9, no. 2, Mar. 2014, pp. 161-6, doi:10.3329/bjp.v9i2.17759.



Research Articles