In vitro anti-venom potential of various Jatropha extracts on neutralizing cytotoxic effect induced by phospholipase A2 of crude venom from Indian cobra (Naja naja)

  • K. V. N. Rathnakar Reddi Departmant of Biotechnology, Acharya Nagarjuna University, Guntur 522 510, AP
  • Sivarathri Siva Rajesh Structural Biology Laboratory, Department of Bioinformatics, School of Chemical and Biotechnology, SASTRA University, Thanjavur 613 401, TN
  • Kumara Narendra Departmant of Biotechnology, Acharya Nagarjuna University, Guntur 522 510, AP
  • Swathi Jangala Departmant of Biotechnology, Acharya Nagarjuna University, Guntur 522 510, AP
  • Puli Chandra Obul Reddy Plant Molecular Biology Laboratory, Department of Botany, School of Life Sciences, Yogi Vemana University, Kadapa 516 003, AP
  • Alapati Krishna Satya Departmant of Biotechnology, Acharya Nagarjuna University, Guntur 522 510, AP
  • Thirunavukkarasu Sivaraman Structural Biology Laboratory, Department of Bioinformatics, School of Chemical and Biotechnology, SASTRA University, Thanjavur 613 401, TN
  • Akila Chandra Sekhar Molecular Genetics and Functional Genomics Laboratory, Department of Biotechnology, School of Life Sciences, Yogi Vemana University, Kadapa 516 003, AP
Keywords: Antidote, Cytotoxicity, Jatropha, PLA2, Snake venom

Abstract

In present study various species of Jatropha were evaluated for antidote nature induced by phospholipase A2 (PLA2) cobra venom. Although qualitative phytochemical analysis exhibited less variation across Jatropha species studied, substantial variability in terms of PLA2 inhibition by various solvent extracts across the species and between different parts of same plant was observed. Among all samples methanolic extracts of J. gossypifolia leaf  showed highest inhibition of PLA2 toxicity while some aqueous extracts of J. foetida and all aqueous extracts of J. curcas, enhanced PLA2 activity. Present results highlight Jatropha not only as rich source of secondary compounds with antidote property for snake bite but also potent toxic agents as revealed with increased hemolysis by some aqueous extracts of J. curcas and J. foetida. Our findings suggest that methanolic leaf extract of J. gossypifolia contain potent small molecular antagonist(s) to the snake venom PLA2 which will be very useful to design adjuvant therapies in treatments of snake bites. 

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Author Biographies

K. V. N. Rathnakar Reddi, Departmant of Biotechnology, Acharya Nagarjuna University, Guntur 522 510, AP
Research scholar
Sivarathri Siva Rajesh, Structural Biology Laboratory, Department of Bioinformatics, School of Chemical and Biotechnology, SASTRA University, Thanjavur 613 401, TN
Research scholar
Kumara Narendra, Departmant of Biotechnology, Acharya Nagarjuna University, Guntur 522 510, AP
Research scholar
Swathi Jangala, Departmant of Biotechnology, Acharya Nagarjuna University, Guntur 522 510, AP
Research scholar
Puli Chandra Obul Reddy, Plant Molecular Biology Laboratory, Department of Botany, School of Life Sciences, Yogi Vemana University, Kadapa 516 003, AP
Assistant Professor
Alapati Krishna Satya, Departmant of Biotechnology, Acharya Nagarjuna University, Guntur 522 510, AP
Assistant Proffessor
Thirunavukkarasu Sivaraman, Structural Biology Laboratory, Department of Bioinformatics, School of Chemical and Biotechnology, SASTRA University, Thanjavur 613 401, TN
Associate Professor

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Published
2014-01-20
How to Cite
Reddi, K. V. N. R., S. Rajesh, K. Narendra, S. Jangala, P. Reddy, A. Satya, T. Sivaraman, and A. Sekhar. “In Vitro Anti-Venom Potential of Various Jatropha Extracts on Neutralizing Cytotoxic Effect Induced by Phospholipase A2 of Crude Venom from Indian Cobra (Naja Naja)”. Bangladesh Journal of Pharmacology, Vol. 9, no. 1, Jan. 2014, pp. 22-28, doi:10.3329/bjp.v9i1.17410.
Section
Research Articles