Antimicrobial activity of methanol extract and fractions from Sarcochlamys pulcherrima

  • Afjal Hussain Mazumder Department of Chemistry, Assam University, Silchar, Assam
  • Jayshree Das Defence Research Laboratory, Tezpur
  • Hemanta Kumar Gogoi Defence Research Laboratory, Tezpur
  • Pronobesh Chattopadhyay Defence Research Laboratory, Tezpur
  • Satya Bhusan Paul Department of Chemistry, Assam University, Silchar, Assam
Keywords: Antimicrobial, n-Butanol fraction, Sarcochlamys pulcherrima

Abstract

Antimicrobial evaluation of methanol extract of Sarcochlamys pulcherrima leaf and its hexane, ethyl acetate, n-butanol and water fractions against 31 strains of microorganisms, using agar well, agar disc diffusion, and broth microdilution methods, revealed the activity of methanol extract against Trichophyton mentagrophytes, Candida albicans, Staphylococcus aureus and Escheracia coli (zone of inhibition: 21-40 mm, 200 mg/mL, and MIC: 6.25-50 mg/mL). All fractions also displayed antimicrobial activity (5-20 mg/mL), indeed ethyl acetate and n-butanol fractions showed better activity (MIC: 0.156 to 2.5 mg/mL). C. albicans was most sensitive to n-butanol fraction (15 mm, 2.5 mg/mL). Ethyl acetate and n-butanol fractions were more active against T. mentagrophytes (12 mm at 1.25 mg/mL) and S. aureus (ethyl acetate-16 mm, n-butanol-14 mm at 0.625 mg/mL). E. coli was inhibited by n-butanol fraction (13 mm at 2.5 mg/mL). Further, n-butanol fraction (400 µg/disc) exhibited promising activity against 14 bacteria, 2 dermatophytes and 2 yeasts strains.

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Published
2014-01-06
How to Cite
Mazumder, A., J. Das, H. Gogoi, P. Chattopadhyay, and S. Paul. “Antimicrobial Activity of Methanol Extract and Fractions from Sarcochlamys Pulcherrima”. Bangladesh Journal of Pharmacology, Vol. 9, no. 1, Jan. 2014, pp. 4-9, doi:10.3329/bjp.v9i1.16760.
Section
Research Articles