Dracorhodin perchlorate induces apoptosis in bladder cancer cells through Bcl-2, Bcl-XL, survivin down-regulation and caspase-3 activation

  • Wei Wei Dental Hospital, Jilin University, Changchun
  • Jun Di Pathology, Jilin Province Hospital, Changchun,
  • Azhar Rasul The Key Laboratory of Molecular Epigenetics of MOE, Institute of Genetics and Cytology, School of Life Sciences, Northeast Normal University, Changchun
  • Chaoyue Zhao Dental Hospital, Jilin University, Changchun
  • Faya Martin Millimouno The Key Laboratory of Molecular Epigenetics of MOE, Institute of Genetics and Cytology, School of Life Sciences, Northeast Normal University, Changchun
  • Ichiro Tsuji Department of Public Health, Tohoku University, Sendai
  • Furhan Iqal Institute of Pure and Applied Biology, Bahauddin Zakariya University, Multan
  • Mahadev Malhi The Key Laboratory of Molecular Epigenetics of MOE, Institute of Genetics and Cytology, School of Life Sciences, Northeast Normal University, Changchun
  • Xiaomeng Li The Key Laboratory of Molecular Epigenetics of MOE, Institute of Genetics and Cytology, School of Life Sciences, Northeast Normal University, Changchun
  • Jiang Li Dental Hospital, Jilin University, Changchun
Keywords: Apoptosis, Bcl-2, Caspase-3, Dracorhodin Perchlorate, T24 cells

Abstract

Urinary bladder cancer is one of the most commonly diagnosed urological malignancies worldwide. Dracorhodin perchlorate, anthocyanin red pigment, has been recently shown to induce apoptotic cell death in several types of cancer cells. However, there is no report elucidating its effect on bladder cancer T24 cells. In this study, for the first time, we investigated the effects of dracorhodin perchlorate on the cell viability and apoptosis in human bladder cancer T24 cells. DNA flow cytometric analysis demonstrated that dracorhodin perchlorate markedly rendered apoptosis of T24 cells in a time-dependent manner. Dracorhodin perchlorate significantly induced the dissipation of mitochondrial membrane potential in T24 cells. Furthermore, dracor-hodin perchlorate-induced apoptosis was regulated by activation of caspase-3 and down-regulation of antiapoptotic proteins, Bcl-2, Bcl-XL, and survivin in T24 cells. These in vitro results suggested that dracorhodin perchlorate should be further examined for in vivo activity and molecular mechanism in human bladder cancer.

Downloads

Download data is not yet available.
Abstract
2405
Download
342 Read
120

Author Biography

Azhar Rasul, The Key Laboratory of Molecular Epigenetics of MOE, Institute of Genetics and Cytology, School of Life Sciences, Northeast Normal University, Changchun

Postdoctoral Research Fellow

References

Adams JM. Ways of dying: Multiple pathways to apoptosis. Genes Dev. 2003; 17: 2481-95.

Adams JM, Cory S. The Bcl-2 apoptotic switch in cancer development and therapy. Oncogene 2007; 26: 1324-37.

Amin AR, Kucuk O, Khuri FR, Shin DM. Perspectives for cancer prevention with natural compounds. J Clin Oncol. 2009; 27: 2712-25.

Burlacu A. Regulation of apoptosis by Bcl-2 family proteins. J Cell Mol Med. 2003; 7: 249-57.

Buytaert E, Dewaele M, Agostinis P. Molecular effectors of multiple cell death pathways initiated by photodynamic therapy. Biochim Biophys Acta. 2007; 1776: 86-107.

Cohen GM. Caspases: The executioners of apoptosis. Biochem J. 1997; 326 ( Pt 1): 1-16.

Cragg GM, Newman DJ. Plants as a source of anti-cancer agents. J Ethnopharmacol. 2005; 100: 72-79.

Danial NN. BCL-2 family proteins: Critical checkpoints of apoptotic cell death. Clin Cancer Res. 2007; 13: 7254-63.

Elmore S. Apoptosis: A review of programmed cell death. Toxicol Pathol. 2007; 35: 495-516.

Fulda S. Evasion of apoptosis as a cellular stress response in cancer. Int J Cell Biol. 2010; 2010: 370835.

He Y, Ju W, Hao H, Liu Q, Lv L, Zeng F. Dracorhodin perchlorate suppresses proliferation and induces apoptosis in human prostate cancer cell line PC-3. J. Huazhong Univ Sci Technolog Med Sci. 2011; 31: 215-19.

Hengartner MO. The biochemistry of apoptosis. Nature 2000; 407: 770-76.

Lawen A. Apoptosis-an introduction. Bioessays 2003; 25: 888-96.

Leist M, Jaattela M. Four deaths and a funeral: From caspases to alternative mechanisms. Nat Rev Mol Cell Biol. 2001; 2: 589-98.

Mallat Z, Tedgui A. Apoptosis in the vasculature: Mechanisms and functional importance. Br J Pharmacol. 2000; 130: 947-62.

Mashima T, Tsuruo T. Defects of the apoptotic pathway as therapeutic target against cancer. Drug Resist Updat. 2005; 8: 339-43.

Newman DJ, Cragg GM. Natural products as sources of new drugs over the last 25 years. J Nat Prod. 2007; 70: 461-77.

Ploeg M, Aben KK, Kiemeney LA. The present and future burden of urinary bladder cancer in the world. World J Urol. 2009; 27: 289-93.

Pommier Y, Sordet O, Antony S, Hayward RL, Kohn KW. Apoptosis defects and chemotherapy resistance: Molecular interaction maps and networks. Oncogene 2004; 23: 2934-49.

Porter AG, Janicke RU. Emerging roles of caspase-3 in apoptosis. Cell Death Differ. 1999; 6: 99-104.

Rao GS, Gerhart MA, Lee RT, Mitscher LA, Drake S. Antimicrobial agents from higher plants. Dragon's blood resin. J Nat Prod. 1982; 45: 646-48.

Rasul A, Bao R, Malhi M, Zhao B, Tsuji I, Li J, Li X. Induction of apoptosis by costunolide in bladder cancer cells is mediated through ROS generation and mitochondrial dysfunction. Molecules 2013; 18: 1418-33.

Rasul A, Ding C, Li X, Khan M, Yi F, Ali M, Ma T. Dracorhodin perchlorate inhibits PI3K/Akt and NF-kappaB activation, up-regulates the expression of p53, and enhances apoptosis. Apoptosis 2012a; 17: 1104-19.

Rasul A, Khan M, Yu B, Ma T, Yang H. Xanthoxyletin, a coumarin induces s phase arrest and apoptosis in human gastric adenocarcinoma SGC-7901 Cells. Asian Pac J Cancer Prev. 2011a; 12: 1219-23.

Rasul A, Yu B, Khan M, Zhang K, Iqbal F, Ma T, Yang H. Magnolol, a natural compound, induces apoptosis of SGC-7901 human gastric adenocarcinoma cells via the mitochondrial and PI3K/Akt signaling pathways. Int J Oncol. 2012b; 40: 1153-61.

Rasul A, Yu B, Yang LF, Ali M, Khan M, Ma T, Yang H. Induction of mitochondria-mediated apoptosis in human gastric adenocarcinoma SGC-7901 cells by kuraridin and Nor-kurarinone isolated from Sophora flavescens. Asian Pac J Cancer Prev. 2011d; 12: 2499-2504.

Rasul A, Yu B, Zhong L, Khan M, Yang H, Ma T. Cytotoxic effect of evodiamine in SGC-7901 human gastric adenocarcinoma cells via simultaneous induction of apoptosis and autophagy. Oncol Rep. 2012c; 27: 1481-87.

Reed JC. Apoptosis-based therapies. Nat Rev Drug Discov. 2002; 1: 111-21.

Siegel R, Naishadham D, Jemal A. Cancer statistics, 2012. CA Cancer J Clin. 2012; 62: 10-29.

Wang X. The expanding role of mitochondria in apoptosis. Genes Dev. 2001; 15: 2922-33.

Xia M, Wang M, Tashiro S, Onodera S, Minami M, Ikejima T. Dracorhodin perchlorate induces A375-S2 cell apoptosis via accumulation of p53 and activation of caspases. Biol Pharm Bull. 2005; 28: 226-32.

Xia MY, Wang MW, Cui Z, Tashiro SI, Onodera S, Minami M, Ikejima T. Dracorhodin perchlorate induces apoptosis in HL-60 cells. J Asian Nat Prod Res. 2006; 8: 335-43.

Published
2013-06-14
How to Cite
Wei, W., J. Di, A. Rasul, C. Zhao, F. Millimouno, I. Tsuji, F. Iqal, M. Malhi, X. Li, and J. Li. “Dracorhodin Perchlorate Induces Apoptosis in Bladder Cancer Cells through Bcl-2, Bcl-XL, Survivin Down-Regulation and Caspase-3 Activation”. Bangladesh Journal of Pharmacology, Vol. 8, no. 3, June 2013, pp. 276-82, doi:10.3329/bjp.v8i3.15025.
Section
Research Articles