Vitamin E levels in buccal cells of arsenicosis patients following vitamin E supplementation

  • Mir Misbahuddin Division of Arsenic Research, Department of Pharmacology, Faculty of Basic Medical Sciences and Paraclinical Sciences, Bangabandhu Sheikh Mujib Medical University, Shahbag, Dhaka http://orcid.org/0000-0003-4667-1005
  • Nadia Farha Division of Arsenic Research, Department of Pharmacology, Faculty of Basic Medical Sciences and Paraclinical Sciences, Bangabandhu Sheikh Mujib Medical University, Shahbag, Dhaka
Keywords: Arsenicosis, Buccal cell, Vitamin E

Abstract

To understand the role of vitamin E in the treatment of arsenical melanosis and keratosis, the buccal cells of 19 patients, 14 arsenic exposed controls and 13 healthy volunteers were collected for the estimation of vitamin E both before and after supplementation with vitamin E (200 IU, caplet) daily orally for 20 weeks. The vitamin E levels in the buccal cells of patients were significantly low in comparison to healthy volunteers (healthy vs patients: 17.2  ±  4.4 vs 12.3 ± 6.1 mg/mg of protein; p=0.012). These low level of vitamin E in patients returned toward normal levels following supplementation with vitamin E for 20 weeks (p=0.044). The vitamin E levels in serum of patients were also low (healthy vs patients: 18.9 ± 4.4 vs 10.2 ± 2.6  mg/mL; p=0.000). Supplementation with vitamin E overcomed the low levels of vitamin E in serum. The cholesterol levels in buccal cells and serum of patients were significantly low in comparison to healthy volunteers (buccal cells of healthy vs patients: 24.5 ± 14.1 vs 10.3 ± 9.8 mg/mg of protein; p=0.005; serum of healthy vs patients: 153.5 ± 22.8 vs 125.3 ± 37.0 mg/dL; p=0.012). After supplementation of vitamin E, there was no significant change in cholesterol levels in both buccal cells and serum of patients.

Downloads

Download data is not yet available.
Abstract
631
Download
441 Read
85

References

Ahmad SA, Faruquee MH, Sayed MHSU, Khan MH, Hadi SA, Khan AW. Chronic arsenic poisoning management by vitamin A, E, C regimen. J Pre Social Med (JOPSOM). 1998; 17: 19-26.

AOAC (Association of Official Analytical Chemists 25. 006) 12th ed. 1975, p 428.

Baker H, Frank O. Determination in serum tocopherol. In: Practical clinical biochemistry. Gowenlock AH.(ed). 6th ed. London, Heinemann Medica books, 1968, p 902.

Bouayed J, Bohn T. Exogenous antioxidants: Double-edged swords in cellular redox state. Oxid Med Cell Longev. 2010: 4: 228-37.

Caussy D. A field guide for detection, management and surveillance of arsenicosis cases. New Delhi, WHO Regional Office for South-East Asia, 2005.

Choudhry ZK, Misbahuddin M, Hosain AKMM, Saleh AA. Inhibitory effect of arsenic on aerobic gut flora in rat. Bangladesh Med Res Counc Bull. 2009; 35: 79-83.

Chowdhury NJA, Mir Misbahuddin M, Rahman MS. Corn extracts lower tissue arsenic level in rat. Bangladesh Med Res Counc Bull. 2009; 35: 21-25.

Chung CJ, Pu YS, Chen YT, Su CT, Wu CC, Shiue HS, Huang CY, Hsueh YM. Protective effects of plasma alpha-tocopherols on the risk of inorganic arsenic-related urothelial carcinoma. Sci Total Environ. 2011; 409: 1039-45.

de Wiele TV, Gallawa CM, Kubachk KM, Creed JT, Basta N, Dayton EA, Whitacre S, Laing GD, Bradham K. Arsenic metabolism by human gut microbiota upon in vitro digestion of contaminated soils. Environ Health Perspect. 2010; 118: 1004-09.

Faizur Rahman MF, Misbahuddin M. Effect of folic acid and tetrahydrofolate on tissue arsenic level in rat. Bangladesh J Pharmacol. 2010; 5: 25-29.

Fariduddin AKM, Misbahuddin M, Manun MIR, Nahar N. Alcohol extract and residue of spirulina in the prevention of accumulation of arsenic in rats. Bangladesh J Physiol Pharmacol. 2001; 17: 15-17.

Hall AF. Arsenical keratosis disappearing with vitamin A therapy. Arch Derm Syph. 1946; 53:154.

Kadirvel R, Sundaram, Mami S, Samuel S, Elango N, Parnneerselvam C. Supplementation of ascorbic acid and alpha-tocopherol prevents arsenic-induced protein oxidation and DNA damage induced by arsenic in rats. Human Exp Toxicol. 2007; 26: 939-46.

Kamaluddin M, Misbahuddin M. Zinc supplement reduces tissue arsenic concentration in rats. Bang Med Res Counc Bull. 2006; 32: 87-91.

Khan MAR, Misbahuddin MS, Khandker S, Ifthaker-Al-Mahmud. Arsenic estimation in foodstuffs of arsenic exposed areas in Bangladesh. In: Applied research on arsenic in Bangladesh. Misbahuddin M (ed). World Health Organization, Dhaka, 2007, pp 31-42.

Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951; 193: 265-75.

Misbahuddin M, Bashar T, Hossain MA. Effectiveness of garlic oil in the treatment of arsenical palmer keratosis. Bangladesh J Pharmacol. 2013: 8: 22-27.

Misbahuddin M, Islam AZ, Khandker S, Ifthaker-Al-Mahmud, Islam N, Anjumanara. Efficacy of spirulina extract plus zinc in patients of chronic arsenicosis poisoning: a randomized placebo-controlled study. Clin Toxicol (Phila). 2006; 44: 135-41.

Misbahuddin M, Kamaluddin M. Simultaneous administration of zinc and arsenic enhances accumulation in tissues. BMJ (rapid response) 14 November 2002.

Misbahuddin M, Khandker S, Jakariya M. Arsenic contamination of drinking water and foodstuffs. In: Drinking water contaminants in Bangladesh: Focuses on arsenic, fluoride, pesticides, manganese and cyanobacteria. Misbahuddin M, Khandker S (eds). Lambert Academic Publishing, Germany, 2011, pp 12-57.

Nabi AHMN, Rahman MM, Islam LN. Evaluation of biochemical changes in chronic arsenic poisoning among Bangladeshi patients. Int J Environ Res Public Health. 2005; 2: 385-93.

Nasir M, Misbahuddin M, Ali SMK. Selenium intervention in reducing arsenic levels in different tissues. In: Bangladesh Environment 2002, Proceedings of the 2nd International Conference on Bangladesh Environment. Ahmed MF, Tanveer SA, Badruzzaman ABM (eds). ICBEN-2002, Dhaka, Bangladesh, 2002, pp 343-52.

Saha B. Effect of ascorbic acid on reduced glutathione level in arsenic-loaded isolated liver tissues of rat. Bangladesh J Pharmacol. 2006; 1: 68-71.

Sinha SK, Misbahuddin M, Ahmed ANN. Factors involved in the development of chronic arsenic poisoning in Bangladesh. Arch Environ Health 2003; 58: 699-700.

Tabassum NE. Effect of alpha-lipoic acid on the removal of arsenic from arsenic-loaded isolated liver tissues of rat. Bangladesh J Pharmacol 2006; 1: 27-32.

Umar BU. Effect of hexane extract of spinach in the removal of arsenic from rat. Bangladesh J Pharmacol. 2007; 2: 27-34.

Wu M, Chiou HY, Wang TW, Hsuch YM, Wang IH, Chen CJ, Lee TC. Association of blood arsenic levels with increased reactive oxidants and decreased antioxidant capacity in a human population of northeastern Taiwan. Environ Health Perspect. 2001; 109: 1011-17.

Yafang S, Gan Z, Jinsong B. Total phenolic content and antioxidant capacity of rice grains with extremely small size. Afr J Agric Res. 2011; 6: 2289-93.

Yousuf AKM, Misbahuddin M, Rahman MS. Secretion of arsenic, cholesterol, vitamin E, and zinc from the site of arsenical melanosis and leucomelanosis in skin. 2011; 49: 374-78.

Published
2013-05-14
How to Cite
Misbahuddin, M., and N. Farha. “Vitamin E Levels in Buccal Cells of Arsenicosis Patients Following Vitamin E Supplementation”. Bangladesh Journal of Pharmacology, Vol. 8, no. 3, May 2013, pp. 236-41, doi:10.3329/bjp.v8i3.14954.
Section
Clinical Trial