Antimicrobial and cytotoxic potentials of Buddleja polystachya extracts


  • Ghada Ahmed Fawzy Department of Pharmacognosy, College of Pharmacy, King Saud University. P.O. Box 22452, Riyadh 11495
  • Ali Ali El Gamal Department of Pharmacognosy, College of Pharmacy, King Saud University. P.O. Box 22452, Riyadh 11495
  • Hanan Yehya Al Ati Department of Pharmacognosy, College of Pharmacy, King Saud University. P.O. Box 22452, Riyadh 11495



Antimicrobial, Buddleja polystachya, Cytotoxicity, Crystal violet assay


Most of the species of Buddleja have found applications in folk medicine. This study aimed to evaluate the in vitro antimicrobial and cytotoxic potentials of B. polystachya extracts. Four extracts were prepared A-D (dichloromethane, ethyl acetate, n-butanol, and aqueous extracts, respectively). The antimicrobial activity was evaluated using the broth micro-dilution assay for minimum inhibitory concentrations (MIC). The crystal violet staining method (CVS) was used for the evaluation of the cytotoxic activity on HepG-2, MCF-7 and HCT-116 human cell lines. Results showed that  the highest antimicrobial activity was given by the ethyl acetate extract followed by the dichloromethane extract, while the n-butanol revealed moderate activity against gram positive bacteria only with no activity against the rest of tested microorganisms. The aqueous extract was totally ineffective against all tested microorganisms at 20 mg/ml. Among the four extracts tested, dichloromethane and ethyl acetate extracts showed the highest cytotoxic activity on all three human cell lines.


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Author Biography

Ghada Ahmed Fawzy, Department of Pharmacognosy, College of Pharmacy, King Saud University. P.O. Box 22452, Riyadh 11495

Assistant Professor


Arif T, Bhosale JD, Kumar N, Mandal TK, Bendre RS, Lavekar GS, Dabur R. Natural products-antifungal agents derived from plants. J Asian Nat Prod Res. 2009; 11: 621-38.

Bakri IM, Douglas CW. Inhibitory effect of garlic extract on oral bacteria. Arch Oral Biol. 2005; 50: 645-51.

Emam AM, Diaz-Lanza AM, Matellano-Fernandez L, Faure R, Moussa AM, Balansard G. Biological activities of Buddelasaponin isolated from Buddleja madagascarensis and Scrophularia scorodonia. Pharmazie 1997; 52: 76-77.

Fridkin SK, Jarvis WR. Epidemiology of nosocomial fungal infection. Clin Microbiol Rev. 1996; 9: 499-511.

Gupta AK, Cooper EA. Update in antifungal therapy of dermatophytosis. Mycopathologia 2008; 166: 353-67.

Gupta P, Murali P, Murali MV, Faridi MMA, Kaul PB, Rama VC, Talwar V. Clinical profile of Klebsiella septicaemia in neonates. Indian J Paediatr. 1993; 60: 565-72.

Houghton PJ. Ethnopharmacology of Some Buddleja Species. J Ethnopharmacol. 1984; 11: 293-308.

Itagaki H, Hagino S, Kato S, Kobayashi T, Umeda M. An in vitro alternative to the draize eye-irritation test: Evaluation of the crystal violet staining method. Toxicol In Vitro. 1991; 5: 139-43.

Jiaoshe L, Yuying Z, Bin W, Jingrong C. Phenylethanoid glucosides from flos Buddlejae. China journal of Chinese Materia Medica. 1997; 22: 613-15.

Mensah AY, Houghton PJ, Bloomfield S, Vlietinck A, Vanden-Berghe D. Known and novel terpenes from Buddleja globosa displaying selective anti-fungal activity against dermatophytes. J Nat Prod. 2000; 63: 121013.

NCCLS (National Committee for Clinical Laboratory Standards). Reference method for broth dilution antifungal susceptibility testing of yeasts. Approved standard M27-A2. Wayne, PA, NCCLS, 2002.

NCCLS (National Committee for Clinical Laboratory Standards). Performance standards for antimicrobial susceptibility testing, 16th informational supplement, M100-S14. Wayne, PA, NCCLS, 2004.

Pardo F, Perich F, Villaroel L, Torres R. Isolation of verbascoside an antimicrobial constituent of Buddleja globosa leaves. J Ethnopharmacol. 1993; 39: 221-22.

Saotome K, Morita H, Umeda M. Cytotoxicity test with simplified crystal violet staining method using microtitre plates and its application to injection drugs. Toxicol In Vitro. 1989; 3: 317-21.

Sarkar R, Mandal N. In vitro cytotoxic effect of hydroalcoholic extracts of medicinal plants on Ehrlichs Ascites Carcinoma (EAC). IJOP. 2011; 3: 370-80.

Zhu L, Tian YJ, Yang L, Jiang JG. Chemical composition and antimicrobial activities of essential oil of Blumea megacephala, EXCLI J. 2011; 10: 62-68.

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How to Cite

Fawzy, G. A., A. A. E. Gamal, and H. Y. Al Ati. “Antimicrobial and Cytotoxic Potentials of Buddleja Polystachya Extracts”. Bangladesh Journal of Pharmacology, vol. 8, no. 2, Mar. 2013, pp. 136-41, doi:10.3329/bjp.v8i2.13844.



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