Evodiamine induces apoptosis in pancreatic carcinoma PANC-1 cells via NF-κB inhibition

Authors

  • Muhammad Khan Central Research Laboratory, Jilin University Bethune Second Hospital, Changchun 130041, P. R. China Department of Zoology, University of the Punjab, Quaid-e-Azam Campus, Lahore 54590, Pakistan
  • Javed Iqbal Qazi Department of Zoology, University of the Punjab, Quaid-e-Azam Campus, Lahore
  • Azhar Rasul Central Research Laboratory, Jilin University Bethune Second Hospital, Changchun
  • Yongchen Zheng Central Research Laboratory, Jilin University Bethune Second Hospital, Changchun
  • Tonghui Ma Central Research Laboratory, Jilin University Bethune Second Hospital, Changchun

DOI:

https://doi.org/10.3329/bjp.v8i1.12802

Keywords:

Apoptosis, Caspase-3, Evodiamine, NF-?B, PANC-1

Abstract

NF-?B constitutively expresses in human pancreatic cancer cells and has been implicated in pancreatic tumorigenesis. Evodiamine, a bioactive alkaloid has been shown to possess antioxidant, anti-inflammatory and antitumor properties. In the present study, the anti-pancreatic cancer potential of evodiamine have been evaluated. Evodiamine effectively inhibited growth and induced apoptosis in PANC-1 cells in a dose-dependent manner. SN50 (NF-?Bp65 inhibitor) significantly increased the evodiamine-induced apoptotic cell death in PANC-1 cells, indicating that NF-?B is involved in evodiamine-induced apoptotic cell death. Furthermore, Western blot analysis showed that evodiamine inhibited the constitutive activation of NF-?Bp65, decreased the expression of anti-apoptotic protein Bcl-2, increased the expression of pro-apoptotic protein Bax and cleaved caspase-3 in PANC-1 cells in a dose-dependent manner. The present study suggests that evodiamine induces apoptosis in PANC-1 cells via NF-?Bp65 inhibition and apoptosis regulators modulation.

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Published

2013-01-01

How to Cite

Khan, M., J. I. Qazi, A. Rasul, Y. Zheng, and T. Ma. “Evodiamine Induces Apoptosis in Pancreatic Carcinoma PANC-1 Cells via NF-κB Inhibition”. Bangladesh Journal of Pharmacology, vol. 8, no. 1, Jan. 2013, pp. 8-14, doi:10.3329/bjp.v8i1.12802.

Issue

Section

Research Articles