Isolation and evaluation of endophytic fungi with antimicrobial ability from Phyllostachys edulis


  • Xiaoye Shen College of Life Science, Capital Normal University, Beijing
  • Dongqi Zheng College of Life Science, Capital Normal University, Beijing
  • Jian Gao SFA Key Laboratory on Bamboo and Rattan Science and Technology, International Center for Bamboo and Rattan, Beijing
  • Chenglin Hou College of Life Science, Capital Normal University, Beijing



antimicrobial activity, Phyllostachys edulis, endophytic fungi, fermentation broth, pathogenic microorganisms


Endophytic fungi (30) isolates from bamboo branches were categorized into 12 genera, based on the blast analyses of ITS nrDNA sequence in GenBank and microscopic examination. The aim of this work was to investigate the antibacterial and antifungal activities of endophytic fungi. Inhibitory effects against clinical pathogens and phytopathogens have been screened for all the isolates preliminarily and strains tentatively identified as Cladosporium sphaerospermum (PE106), Simplicillium lanosoniveum (PE120), Curvularia sp. (PE127), Didymella sp. (PE128) and Penicillium cf. raistrickii (PE130) presented bioactivity against at least four tested pathogens using the agar diffusion method. Crude extracts of PE106, PE120, PE127 and PE130 displayed broad-spectrum activity against plant pathogenic fungi by mycelial radial growth test. All of the four isolates were found to have high bioactivity against the frequent plant pathogenic fungus Botryotinia fuckeliana, and two of the isolates (PE120 and PE130) also inhibited the growth of phytopathogen Thanatephorus cucumeris noteworthily. This study is the first report on the antimicrobial activity of endophytic fungi associated with branches of Ph. edulis.


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Ali SM, Olivo M. Efficacy of hypocrellin pharmacokinetics in phototherapy. Int J Oncol. 2002; 21: 1229-37.

Azevedo JL, Maccheroni Jr W, Pereira JO, De Arajo WL. Endophytic microorganisms: a review on insect control and recent advances on tropical plants. Electron J Biotechn. 2000; 3: 15-16.

Banerjee U. Production of β-glucosidase (cellobiase) by Curvularia sp. Lett Appl Microbiol. 1990; 10: 197-99.

Banerjee U, Vohra R. Production of laccase by Curvularia sp. Folia Microbiol. 1991; 36: 343-46.

Boonphong S, Kittakoop P, Isaka M, Pittayakhajonwut D, Tanticharoen M, Thebtaranonth Y. Multiplolides A and B, new antifungal 10-membered lactones from Xylaria multiplex. J Nat Prod. 2001; 64: 965-67.

Braun U, Crous PW, Dugan F, Groenewald JZE, Sybren De Hoog G. Phylogeny and taxonomy of Cladosporium-like hyphomycetes, including Davidiella gen. nov., the teleomorph of Cladosporium s. str. Mycol Prog. 2003; 2: 3-18.

Cai L, Zhang K, McKenzie EHC, Hyde KD. Freshwater fungi from bamboo and wood submerged in the Liput River in the Philippines. Fungal Divers. 2003; 13: 1-12.

Carroll G. Fungal endophytes in stems and leaves: From latent pathogen to mutualistic symbiont. Ecology 1988; 2-9.

Carroll G. Forest endophytes: Pattern and process. Can J Bot. 1995; 73: 1316-24.

Chin W, Lau W, Cheng C, Olivo M. Evaluation of Hypocrellin B in a human bladder tumor model in experimental photodynamic therapy: biodistribution, light dose and drug-light interval effects. Int J Oncol. 2004; 25: 623-29.

de Medeiros LS, Murgu M, de Souza AQL, Rodrigues-Fo E. Antimicrobial depsides produced by Cladosporium uredinicola, an endophytic fungus isolated from Psidium guajava fruits. Helv Chim Acta. 2011; 94: 1077-84.

de Siqueira VM, Conti R, de Araujo JM, Souza-Motta CM. Endophytic fungi from the medicinal plant Lippia sidoides Cham. and their antimicrobial activity. Symbiosis 2011; 53: 89-95.

Deininger MH, Weinschenk T, Morgalla MH, Meyermann R, Schluesener HJ. Release of regulators of angiogenesis following Hypocrellin-A and -B photodynamic therapy of human brain tumor cells. Biochem Biophys Res Commun. 2002; 298: 520-30.

Faeth SH, Fagan WF. Fungal endophytes: Common host plant symbionts but uncommon mutualists. Integr Comp Biol. 2002; 42: 360-68.

Gardes M, Bruns TD. ITS primers with enhanced specificity for basidiomycetes: Application to the identification of mycorrhizae and rusts. Mol Ecol. 1993; 2: 113-18.

Halvorson JJ, Cassida KA, Turner KE, Belesky DP. Nutritive value of bamboo as browse for livestock. Renew Agric Food Syst. 2011; 26: 161-70.

Hamayun M, Khan S, Ahmad N, Tang DS, Kang SM, Na CI, Sohn EY, Hwang YH, Shin DH, Lee BH, Kim JG, Lee IJ. Cladosporium sphaerospermum as a new plant growth-promoting endophyte from the roots of Glycine max (L.) Merr. World J Microb Biot. 2009; 25: 627-32.

Honda Y, Ueki M, Okada G, Onose R, Usami R, Horikoshi K, Osada H. Isolation, and biological properties of a new cell cycle inhibitor, curvularol, isolated from Curvularia sp. RK97-F166. J Antibiot. 2001; 54: 10-10.

Hormazabal E, Piontelli E. Endophytic fungi from Chilean native gymnosperms: Antimicrobial activity against human and phytopathogenic fungi. World J Microb Biot. 2009; 25: 813-19.

Hyde K, Ho W, McKenzie E, Dalisay T. Saprobic fungi on bamboo culms. Fungal Divers. 2001; 7: 35-48.

Keski-Saari S, Ossipov V, Julkunen-Tiitto R, Jia JB, Danell K, Veteli T, Zhang GQ, Xiong YW, Niemela P. Phenolics from the culms of five bamboo species in the Tangjiahe and Wolong Giant Panda Reserves, Sichuan, China. Biochem Syst and Ecol. 2008; 36: 758-65.

Kocisova E, Jancura D, Sanchez-Cortes S, Miskovsky P, Chinsky L, Garcia-Ramos JV. Interaction of antiviral and antitumor photoactive drug hypocrellin A with human serum albumin. J Biomol Struct Dyn. 1999; 17: 111-20.

Koide CL, Collier AC, Berry MJ, Panee J. The effect of bamboo extract on hepatic biotransforming enzymes: Findings from an obese-diabetic mouse model. J Ethnopharmacol. 2011; 133: 37-45.

Li XM, Gao J, Yue YD, Hou CL. Studies on systematics, biology and bioactive substance of Shiraia bambusicola. Forest Res. 2009; 22: 279-84.

Liu B. In: Chinese Medical Fungi. Liu B. Taiyuan, China, Shanxi People Press, 2001.

Lu B, Wu X, Tie X, Zhang Y. Toxicology and safety of anti-oxidant of bamboo leaves. Part 1: Acute and subchronic toxicity studies on anti-oxidant of bamboo leaves. Food Chem Toxicol. 2005; 43: 783-92.

Lu B, Wu X, Shi J, Dong Y, Zhang Y. Toxicology and safety of antioxidant of bamboo leaves. Part 2: Developmental toxicity test in rats with antioxidant of bamboo leaves. Food Chem Toxicol. 2006; 44: 1739-43.

Ma G, Khan SI, Jacob MR, Tekwani BL, Li Z, Pasco DS, Walker LA, Khan IA. Antimicrobial and antileishmanial activities of hypocrellins A and B. Antimicrob Agents Chemother. 2004; 48: 4450-52.

Ma Y, Chang Z, Zhao J, Zhou M. Antifungal activity of Penicillium striatisporum Pst10 and its biocontrol effect on Phytophthora root rot of chilli pepper. Biol Control. 2008; 44: 24-31.

Mazzini S, Merlini L, Mondelli R, Scaglioni L. Conformation and tautomerism of hypocrellins: Revised structure of shiraia chrome A. J Chem Soc Perkin Trans. 2001; 2: 409-16.

Mejia AI, Gallardo C, Vallejo JJ, Ramirez G, Arboleda C, Durango ES, Jaramillo FA, Cadavid E. Plants of the genus bambusa: Importance and application in the pharmaceutical, cosmetic and food indusry. Vitae-Columbia. 2009; 16: 396-405.

Mirossay A, Mojzis J, Tothova J, Hajikova M, Lackova A, Mirossay L. Hypocrellin and hypericin-induced phototoxicity of HL-60 cells: Apoptosis or necrosis? Phytomedicine 2000; 7: 471-76.

Mohanan C. In: Diseases of of bamboos in Asia: an illustrated manual. Mohanan C. Kerala, India, Brill Academic Publishers, 2002.

Morakotkarn D, Kawasaki H, Seki T. Molecular diversity of bamboo associated fungi isolated from Japan. Fems Microbiol Lett. 2007; 266: 10-19.

Morakotkarn D, Kawasaki H, Tanaka K, Okane I, Seki T. Taxonomic characterization of Shiraia-like fungi isolated from bamboos in Japan. Mycoscience 2008; 49: 258-65.

Nicoletti R, Lopez-Gresa MP, Manzo E, Carella A, Ciavatta ML. Production and fungitoxic activity of Sch 642305, a secondary metabolite of Penicillium canescens. Mycopathologia 2007; 163: 295-301.

Panee J, Liu W, Lin Y, Gilman C, Berry MJ. A novel function of bamboo extract in relieving lipotoxicity. Phytother Res. 2008; 22: 675-80.

Park YS, Kim KC, Lee JH, Cho SM, Choi YS, Kim YC. Cladosporium sp. is the major causal agent in the microbial complex associated with the skin sooty dapple disease of the asian pear in Korea. Plant Pathol J. 2008; 24: 118-24.

Saikkonen K, Faeth S, Helander M, Sullivan T. Fungal endophytes: A continuum of interactions with host plants. Annu Rev Ecol Syst. 1998; 319-43.

Seto Y, Kogami Y, Shimanuki T, Takahashi K, Matsuura H, Yoshihara T. Production of phleichrome by Cladosporium phlei as stimulated by diketopiperadines of Epichloe typhina. Biosci Biotech Bioch. 2005; 69: 1515-19.

Stierle A, Strobel G, Stierle D. Taxol and taxane production by Taxomyces andreanae, an endophytic fungus of Pacific yew. Science 1993; 260: 214-16.

Stierle A, Strobel G, Stierle D, Grothaus P, Bignami G. The search a taxol-producing microorganism among the endophytic fungi of the pacitic yew, taxus-brevifolia. J Nat Prod. 1995; 58: 1315-24.

Su YJ, Yin XY, Rao SQ, Cai YJ, Reuhs B, Yang YJ. Natural colourant from Shiraia bambusicola: Stability and antimicrobial activity of hypocrellin extract. Int J Food Sci Tech. 2009; 44: 2531-37.

Teles HL, Silva GH, Castro-Gamboa I, Bolzani VS, Pereira JO, Costa-Neto CM, Haddad R, Eberlin MN, Young MCM, Araújo ÂR. Benzopyrans from Curvularia sp., an endophytic fungus associated with Ocotea corymbosa (Lauraceae). Phytochemistry. 2005; 66: 2363-67.

Trisuwan K, Rukachaisirikul V, Phongpaichit S, Preedanon S, Sakayaroj J. Modiolide and pyrone derivatives from the sea fan-derived fungus Curvularia sp PSU-F22. Arch Pharm Res. 2011; 34: 709-14.

Umali T, Quimio T and Hyde KD. Endophytic fungi in leaves of Bambusa tuldoides. Fungal Sci. 1999; 14: 11-18.

Varma GB, Fatope MO, Marwah RG, Deadman ME, Al-Rawahi FK. Production of phenylacetic acid derivatives and 4-epiradicinol in culture by Curvularia lunata. Phytochemistry. 2006; 67: 1925-30.

Wan X, Chen Y. Hypocrellin A, a new drug for photochemotherapy. Kexue Tongbao. 1981; 26: 1040-42.

Wang FW, Jiao RH, Cheng AB, Tan SH, Song YC. Antimicrobial potentials of endophytic fungi residing in Quercus variabilis and brefeldin A obtained from Cladosporium sp. World J Microb Biot. 2007; 23: 79-83.

Wang ZJ, He YY, Huang CG, Huang JS, Huang YC, An JY, Gu Y, Jiang LJ. Pharmacokinetics, tissue distribution and photodynamic therapy efficacy of liposomal-delivered hypocrellin A, a potential photosensitizer for tumor therapy. Photochem Photobiol. 1999; 70: 773-80.

White TJ, Bruns T, Lee S, Taylor J. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: PCR protocols: A guide to methods and applications. Innis MA, Gelfand DH, Sninsky JJ, White TJ, 1st ed. San Diego, USA, Academic Press, 1990, pp 315-22.

Wilson D. Ecology of woody plant endophytes. In: Microbial endophytes. Bacon CW, White JF Jr, eds., 1st ed. New York, USA, CRC Press, 2001, pp 389-420.

Xu S, Chen S, Zhang M, Shen T, Liu Z, Zhao Y, Wu Y. Cyclohexylamino-demethoxy-hypocrellin B and photodynamic therapy decreases human cancer in vitro. Anticancer Drug Des. 2001; 16: 271-77.

Yang H, Wu T, Zhang M, Zhang Z. A novel photosensitizer, 2-butylamino-2-demethoxy-hypocrellin B (2-BA-2-DMHB) - its photodynamic effects on HeLa cells: Efficacy and apoptosis. Biochim Biophys Acta. 2001; 1540: 22-31.

Yu H, Zhang L, Li L, Zheng C, Guo L, Li W, Sun P, Qin L. Recent developments and future prospects of antimicrobial metabolites produced by endophytes. Microbiol Res. 2010; 165: 437-49.

Zhang P, Zhou PP, Yu LJ. An endophytic taxol-producing fungus from taxus media, Cladosporium cladosporioides MD2. Curr Microbiol. 2009; 59: 227-32.

Zhou Z, Yang H, Zhang Z. Role of calcium in phototoxicity of 2-butylamino-2-demethoxy-hypocrellin A to human gastric cancer MGC-803 cells. Biochim Biophys Acta. 2003; 1593: 191-200.

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How to Cite

Shen, X., D. Zheng, J. Gao, and C. Hou. “Isolation and Evaluation of Endophytic Fungi With Antimicrobial Ability from Phyllostachys Edulis”. Bangladesh Journal of Pharmacology, vol. 7, no. 4, Oct. 2012, pp. 249-57, doi:10.3329/bjp.v7i4.12068.



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