Isolation and evaluation of endophytic fungi with antimicrobial ability from Phyllostachys edulis

  • Xiaoye Shen College of Life Science, Capital Normal University, Beijing
  • Dongqi Zheng College of Life Science, Capital Normal University, Beijing
  • Jian Gao SFA Key Laboratory on Bamboo and Rattan Science and Technology, International Center for Bamboo and Rattan, Beijing
  • Chenglin Hou College of Life Science, Capital Normal University, Beijing
Keywords: antimicrobial activity, Phyllostachys edulis, endophytic fungi, fermentation broth, pathogenic microorganisms

Abstract

Endophytic fungi (30) isolates from bamboo branches were categorized into 12 genera, based on the blast analyses of ITS nrDNA sequence in GenBank and microscopic examination. The aim of this work was to investigate the antibacterial and antifungal activities of endophytic fungi. Inhibitory effects against clinical pathogens and phytopathogens have been screened for all the isolates preliminarily and strains tentatively identified as Cladosporium sphaerospermum (PE106), Simplicillium lanosoniveum (PE120), Curvularia sp. (PE127), Didymella sp. (PE128) and Penicillium cf. raistrickii (PE130) presented bioactivity against at least four tested pathogens using the agar diffusion method. Crude extracts of PE106, PE120, PE127 and PE130 displayed broad-spectrum activity against plant pathogenic fungi by mycelial radial growth test. All of the four isolates were found to have high bioactivity against the frequent plant pathogenic fungus Botryotinia fuckeliana, and two of the isolates (PE120 and PE130) also inhibited the growth of phytopathogen Thanatephorus cucumeris noteworthily. This study is the first report on the antimicrobial activity of endophytic fungi associated with branches of Ph. edulis.

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Published
2012-10-28
How to Cite
Shen, X., D. Zheng, J. Gao, and C. Hou. “Isolation and Evaluation of Endophytic Fungi With Antimicrobial Ability from Phyllostachys Edulis”. Bangladesh Journal of Pharmacology, Vol. 7, no. 4, Oct. 2012, pp. 249-57, doi:10.3329/bjp.v7i4.12068.
Section
Research Articles