Alpha-glucosidase inhibitory effect and enzyme kinetics of coastal medicinal plants

  • Selvaraj Gurudeeban Marine Medicinal Plant Biotechnology Division, Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai
  • Kaliyamurthi Satyavani Marine Medicinal Plant Biotechnology Division, Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai
  • Thirugnanasambandam Ramanathan Marine Medicinal Plant Biotechnology Division, Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai
Keywords: Aegle marmelos, alpha-Glucosidase inhibitor, Citrullus colocynthis, Diabetes type 2, Postprandial hyperglycemia

Abstract

Numerous metabolic disorders and diseases associated with the glucosidase enzyme activity. Therefore, in the present study we evaluated alpha glucosidase inhibitory effect of coastal medicinal plants on Saccharomyces cerevisiae alpha-glucosidase to control postprandial hyperglycemia. The n-hexane extracts of study plants screened for alpha-glucosidase inhibitory activity using yeast glucosidase. The enzyme kinetics studied Line weaver burk plot method. Among the ten plants Citrullus colocynthis, Aegle marmelos and Ipomoea pescaprae exhibited potent alpha-glucosidase inhibitory activity, 85.9 ±0.10, 72.23 ± 0.30 and 67.9 ± 0.11 respectively. From the enzyme kinetics assay potent plants contain uncompetitive inhibitors of alpha glucosidase. C. colocynthis, A. marmelos and Ipomoea pescaprae have potential alpha-glucosidase inhibitors that can exploited for its used to treat diabetes.

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Published
2012-09-23
How to Cite
Gurudeeban, S., K. Satyavani, and T. Ramanathan. “Alpha-Glucosidase Inhibitory Effect and Enzyme Kinetics of Coastal Medicinal Plants”. Bangladesh Journal of Pharmacology, Vol. 7, no. 3, Sept. 2012, pp. 186-91, doi:10.3329/bjp.v7i3.11499.
Section
Research Articles