Alpha-glucosidase inhibitory effect and enzyme kinetics of coastal medicinal plants
Numerous metabolic disorders and diseases associated with the glucosidase enzyme activity. Therefore, in the present study we evaluated alpha glucosidase inhibitory effect of coastal medicinal plants on Saccharomyces cerevisiae alpha-glucosidase to control postprandial hyperglycemia. The n-hexane extracts of study plants screened for alpha-glucosidase inhibitory activity using yeast glucosidase. The enzyme kinetics studied Line weaver burk plot method. Among the ten plants Citrullus colocynthis, Aegle marmelos and Ipomoea pescaprae exhibited potent alpha-glucosidase inhibitory activity, 85.9 ±0.10, 72.23 ± 0.30 and 67.9 ± 0.11 respectively. From the enzyme kinetics assay potent plants contain uncompetitive inhibitors of alpha glucosidase. C. colocynthis, A. marmelos and Ipomoea pescaprae have potential alpha-glucosidase inhibitors that can exploited for its used to treat diabetes.
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Copyright (c) 2012 Selvaraj Gurudeeban, Kaliyamurthi Satyavani, Thirugnanasambandam Ramanathan
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