In vitro anthelmintic effects of Artemisia afra and Mentha longifolia against parasitic gastro-intestinal nematodes of livestock


  • Nthatisi Innocentia Molefe Parasitology Research Program, Department of Zoology and Entomology, University of the Free State - Qwaqwa Campus, Phuthaditjhaba
  • Ana Mbokeleng Tsotetsi Parasitology Research Program, Department of Zoology and Entomology, University of the Free State - Qwaqwa Campus, Phuthaditjhaba and Parasites, Vectors and Vector-borne diseases, ARC-Onderstepoort Veterinary Institute, Onderstepoort
  • Anofi Omotayo Tom Ashafa Department of Plant Sciences, University of the Free State ? Qwaqwa Campus, Phuthaditjhaba
  • Oriel Matlhahane Molefi Thekisoe Parasitology Research Program, Department of Zoology and Entomology, University of the Free State - Qwaqwa Campus, Phuthaditjhaba



Artemisia afra, Mentha longifolia, gastrointestinal nematodes, anthelmintic activity, egg hatch, larval development, larval mortality.


Gastrointestinal nematodes are the most widespread and economically important class of the helminths. The major threat posed by nematode infections is the productivity loss due to their ability to disrupt nutrient utilization of the livestock resulting in hunger and malnutrition. Acetone and aqueous extracts were prepared from Mentha longifolia and Artemisia afra and tested on egg hatch, larval development and larval mortality assays at three concentrations 2.5, 5.0 and 7.5 mg/mL. Certain percentages of egg hatch and larval mortality were inhibited by both plants; however, larval development was totally inhibited at all concentrations as compared to the negative control. Generally the acetone extracts from the two plants were most effective against parasitic gastrointestinal nematodes of livestock. This report therefore validates the folkloric use of these species in the management of internal parasites by the Basotho of the eastern Free State Province of South Africa.


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How to Cite

Molefe, N. I., A. M. Tsotetsi, A. O. T. Ashafa, and O. M. M. Thekisoe. “In Vitro Anthelmintic Effects of Artemisia Afra and Mentha Longifolia Against Parasitic Gastro-Intestinal Nematodes of Livestock”. Bangladesh Journal of Pharmacology, vol. 7, no. 3, Aug. 2012, pp. 157-63, doi:10.3329/bjp.v7i3.11437.



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