Evaluation of in vitro anthelmintic activities of leaf and stem extracts of Justicia gendarussa

  • Monika Rani Saha Department of Pharmacy, Noakhali Science and Technology University, Noakhali
  • Pankaj Chandra Debnath Department of Pharmacy, Noakhali Science and Technology University, Noakhali
  • Md. Ajijur Rahman Department of Pharmacy, Rajshahi University, Rajshahi
  • Md. Anwar Ul Islam Department of Pharmacy, Rajshahi University, Rajshahi
Keywords: Acanthaceae, Anthelmintic activity, Justicia gendarussa

Abstract

This present study explored the anthelmintic activities of crude methanolic extract of dried leaves and stems of Justicia gendarussa, an evergreen plant belonging to the family Acanthaceae. This plant is commonly known as Bishjarul in Bangladesh. Five concentrations (10, 20, 30, 40 and 50 mg/mL) of each extract were studied in the bioassay to determine the paralysis time and death time of the worm, Pheretima posthuma. Methanolic extract of leaves (50 mg/mL) caused paralysis of the worms  at 35.33 min and death at 70.67 min while methanolic extract of stem of J. gendarussa caused paralysis  at 41.33 min and death at 89.33 min. Albendazole (positive control) paralyzed  and killed the worms  at 17 min and 48 min, respectively. The study confirms the significant anthelmintic activities of leaves and stems extract of J. gendarussa and therefore demands the isolation of active principles thorough bioassay. This is the first report of anthelmintic activities of J. gendarussa.

Downloads

Download data is not yet available.
Abstract
1772
Download
806 Read
303

References

Agarwal R, Kharya MD, Srivastava R. Antimicrobial and anthelmintic activities of the essential oil of Nigella sativa Linn. Indian J Expt Biol. 1979; 17: 1264.

Ajaiyeoba EO, Onocha PA, Olarenwaju OT. In vitro anthelmintic properties of Buchholzia coriaceae and Gynandropsis gynandries extract. Pharm Biol. 2001; 39: 217-20.

Akhtar MS, Iqbal Z, Khan MN, Lateef M. Anthelmintic activity of medicinal plants with particular reference to their use in animals in Indo-Pakistan subcontinent. Small Ruminants Res. 2000; 38: 99107.

Ali SM, Mehta RK. Preliminary pharmacological and anthelmintic studies of the essential oil of Piper betle L. Indian J Pharm. 1970; 32: 13233.

Banerjee A, Nigam SS. In vitro anthelmintic activity of the essential oils derived from the various species of the genus Curcuma. Sci. Cult. 1978; 44: 50304.

Chakravarty AJ, Dastidar PPG, Pakrashi SC. Simple Aromatic Amines from Justicia Gendarussa: 13C NMR Spectra of the bases and their analogues. Tetrahedron. 1982; 38: 1797-802.

Chatterjee KD. Parasitology, protozoology and helminthology. 6th ed. Calcutta: In Guha Ray Sree Saraswaty Press Ltd; 1967.

Chin YW, Balunas MJ, Chai HB, Kinghorn AD. Drug discovery from natural sources. AAPS J. 2006; 8: E239-53.

Dash GK, Suresh P, Kar DM, Ganpaty S, Panda SB. Evaluation of Evolvulus alsinoids Linnb for anthelmintic and antimicrobial activities. J Nat Rem. 2002; 2: 182-85.

Dengre SL. Chemical and physiological examination of essential oils from Indian sources. Ph.D. Thesis. Dr. Hari Singh Gour Vishwavidyalaya, Sagar, India, 1982, pp 171-79.

Dixit VK and Varma KC , Anthelmintic properties of essential oils from rhizomes of Hedychium coronarium Koenig and Hedychium spicatum Koenig. Indian J Pharm. 1975; 37: 143-44.

Dubey NK, Kumar R, Tripathi P. Global promotion of herbal medicine: India's opportunity. Curr Sci. 2004; 86: 37-41.

Gaind KN, Budhiraja RD. Antibacterial and anthelmintic activity of Withania coagulans Dunal. Indian J Pharm. 1967; 29: 185-86.

Garg SC, Kasera HL. Anthelmintic activity of Callistemon viminalis. Fitoterapia. 1982; 53: 179-81.

Garg SC, Nakhare S. Studies on the essential oils from the flowers of Eupatorium triplinerve. Indian Perfumer. 1993; 37: 318-23.

Garg SC, Siddiqui N. Anthelmintic activity of Vernonia teres L. and Clerodendrum phlomidis. J Res Edu Indian Med. 1992; 11: 13.

Garg SC, Kasera HL. In vitro anthelmintic activity of the essential oil of Anacardium occidentale. Indian Perfumer. 1982; 26: 239-40.

Geert S, Dorny P. Anthelmintic resistance in helminthes of animals of man in the tropics. Bulletin-des-Seances, Academic- Royale-des-Sciencesd. 1995; 3: 401-23.

Girgune JB, Jain NK, Garg BD. Antimicrobial and anthelmintic activity of essential oil from Gardenia lucida Roxb. Indian Perfumer. 1979; 23: 213-15.

Girgune JB, Jain NK, Garg B. Anthelmintic activity of some essential oils. Indian Perfumer. 1978; 22: 296-97.

Jain ML, Jain SR. Therapeutic Utility of Ocimum basilicum var. album. Planta Medica. 1972; 22: 66-70.

Kokate CK, Varma KC. Anthelmintic activity of some essential oils. Indian J Hosp Pharm. 1971; 8: 150-51.

Kumar A, Lakshman K, Jayaveera KN, Nandeesh R, Manoj B, Ranganayakulu D. Comparative in vitro anthelmintic activity of three plants from the Amaranthaceae family, Archives Biol Sci. 2010; 62: 185-89.

Kumar BSA, Lakshman K, Jayaveera KN, Velmurugan C, Manoj B, Sridhar SM. Anthelmintic activity of methanol extract of Amaranthus caudatus Linn. Internet J Food Safety. 2010; 12: 127-29.

Lewis WH, Lewis. Medicinal botany plants affecting mans health. New York, John Wiley & Sons, 1977.

Marley CL. The effect of dietary forage on the development and survival of helminth parasites in ovine faeces. Vet Parasitol. 2003; 118: 93-107.

Mehta, MB, Kharya MD, Srivastava R, Varma KC. Antimicrobial and anthelmintic activities of the essential oil of Zanthoxylurn alatum Roxb. Indian Perfumer. 1981; 25: 1-3.

Mishra SH, Gaud RS, Sharma RA, Chaturvedi SC. Anthelmintic activity of some essential oils. Indian Perfumer. 1979; 23: 208-09.

Mrunthunjaya K, Hukkeri VI. Antioxidant and free radical scavenging potential of J. gendarussa Burm, leaves in vitro. Nat. Prod. Sci. 2007; 3: 199-206.

Nadkarni AK. Indian Materia Medica. 3rd ed. Bombay, Popular Prakashan, 1954.

Nanda A, Iyengar MA, Narayan CS, Kalkarni DR. Investigations on the root bark of Aglaia odoratissima. Fitoterapia. 1987; 58: 189-91.

Niezen JH, WaghornTS, Charleston WAG, Waghorn GC. Growth and gastrointestinal nematode parasitism in lambs grazing either lucerne (Medicago sativa) or sulla (Hedysarum coronarium) which contain condensed tannins. J Agric Sci. 1995; 125: 281-89.

Rantnasooriya WD, Derianyagala SA, Dehigas DC. Antinociceptive activity and toxicological study of aqueous leaves extract of J gendarussa. Phcog Mag. 2007; 3: 145-55.

Reddy LJ, Jose B, Anjana JC, Ruveena TN. Evaluation of antibacterial activity of Trichosanthes Cucumerina L. and Cassia didymobotrya Fresh leaves. Int J Pharm Pharm Sci. 2010; 2: 153-55.

Said M. Hamdard Pharmacopea of Eastern Medicine. Karachi, Hamdard National Foundation, 1969.

Satyavati GV, Raina MK, Sharma M. Medicinal plants of India. New Delhi, Indian Council of Medical Research, 1976, pp 20106.

Siddiqui N, Garg S. In vitro anthelmintic activity of some essential oils. Pakistan J Sci Indust Res. 1990; 33: 53637.

Sollman T. Anthelmintic: Their efficacy as tested on earthworms. J Pharmacol Exp Ther. 1918; 112: 129-70.

Szewezuk VD, Mongelli ER, Pomilio AB. Antiparasitic activity of Melia azadirach growing in Argentina. Molecular Med Chem. 2003;1: 54-57.

Thorn GW, Adams RD, Braunwald E, Isselbacher KJ, Petersdorf RG. Harrison's Principles of internal medicine. New York, McGraw Hill Co., 1977.

Uddin MR, Sinha S, Hossain MA, Kaisar MA, Hossain MK, Rashid MA. Chemical and biological investigations of Justicia gendarussa (Burm. f). Dhaka Univ J Pharm Sci. 2011; 10: 53-57.

Vidyarthi RD. A textbook of Zoology. 14th ed. New Delhi, S. Chand and Co, 1967.

Published
2012-05-12
How to Cite
Saha, M., P. Debnath, M. Rahman, and M. Islam. “Evaluation of in Vitro Anthelmintic Activities of Leaf and Stem Extracts of Justicia Gendarussa”. Bangladesh Journal of Pharmacology, Vol. 7, no. 1, May 2012, pp. 50-53, doi:10.3329/bjp.v7i1.10558.
Section
Research Articles