Hydroxysafflor yellow A suppresses oxidized low density lipoprotein induced proliferation of vascular smooth muscle cells

  • Lin Sheng Department of Cardiology, the 2nd Hospital of Shandong University, Jinan, Shandong 250033
  • Shaojie Bi Department of Cardiology, the 2nd Hospital of Shandong University, Jinan, Shandong 250033
  • Chao Cheng Department of Cardiology, the 2nd Hospital of Shandong University, Jinan, Shandong 250033
  • Jingbo Zhang Department of Cardiology, the 2nd Hospital of Shandong University, Jinan, Shandong 250033
Keywords: Hydroxysafflor yellow A, Oxidized low density lipoprotein, Mitogen activated protein kinase phospholipase 1, Smooth muscle cell

Abstract

To investigate the relationship between the suppression of Hydroxysafflor yellow A (HSYA) on the oxidized low density lipoprotein (ox-LDL) induced proliferation of vascular smooth muscle cells (VSMCs) and the mRNA and protein expression of extracellular signal-regulated protein kinase 1/2 (ERK1/2) and mitogen activated protein kinase phospholipase-1 (MAKP-1), VSMCs were treated with HSYA at 10 ?mol/L and/or ox-LDL at 35 mg/L for 48 h. MTT assay was done to measure cell survival rate, flow cytometry to detect cell cycle, reverse transcription PCR and Western blot to detect the expression of ERK1/2 and MAKP-1. When compared to cells treated with ox-LDL alone, the survival rate of cells treated with two reagents was reduced and the proportion of cells in G0/G1 phase significantly increased, with increased MKP-1 expression. The study suggests HSYA can inhibit VSMC proliferation via increasing MKP-1 expression, reducing p-ERK1/2 activity and suppressing cell cycle.

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References

Brunt KR, Fenrich KK, Kiani G, Tse MY, Pang SC, Ward CA, Melo LG. Protection of human vascular smooth muscle cells from H2O2-induced apoptosis through functional codependence between HO-1 and AKT. Arterioscler Thromb Vasc Biol. 2006; 26: 2027-34.

Chahine MN, Blackwood DP, Dibrov E, Richard MN, Pierce GN. Oxidized LDL affects smooth muscle cell growth through MAPK-mediated actions on nuclear protein import. J Mol Cell Cardiol. 2009; 46: 431-41.

Curcio A, Torella D, Indolfi C. Mechanisms of smooth muscle cell proliferation and endothelial regeneration after vascular injury and stenting. Circ J. 2011; 75: 1287-96.

Imazu M, Ono K, Tadehara F, Kajiwara K, Yamamoto H, Sumii K, Tasaki N, Oiwa J, Shimohara Y, Gomyo Y, Itabe H. Plasma levels of oxidized low density lipoprotein are associated with stable angina pectoris and modalities of acute coronary syndrome. Int Heart J. 2008; 49: 515-24.

Li Y, Lévesque LO, Anand-Srivastava MB. Epidermal growth factor receptor transactivation by endogenous vasoactive peptides contributes to hyperproliferation of vascular smooth muscle cells of SHR. Am J Physiol Heart Circ Physiol. 2010; 299: H1959-67.

Naruko T, Ueda M, Ehara S, Itoh A, Haze K, Shirai N, Ikura Y, Ohsawa M, Itabe H, Kobayashi Y, Yamagishi H, Yoshiyama M, Yoshikawa J, Becker AE. Persistent high levels of plasma oxidized low-density lipoprotein after acute myocardial infarction predict stent restenosis. Arterioscler Thromb Vasc Biol. 2006; 26: 877-83.

Perez J, Torres RA, Rocic P, Cismowski MJ, Weber DS, Darley-Usmar VM, Lucchesi PA. PYK2 signaling is required for PDGF-dependent vascular smooth muscle cell proliferation. Am J Physiol Cell Physiol. 2011; 301: C242-51.

Published
2012-06-04
How to Cite
Sheng, L., S. Bi, C. Cheng, and J. Zhang. “Hydroxysafflor Yellow A Suppresses Oxidized Low Density Lipoprotein Induced Proliferation of Vascular Smooth Muscle Cells”. Bangladesh Journal of Pharmacology, Vol. 7, no. 2, June 2012, pp. 87-93, doi:10.3329/bjp.v7i2.10499.
Section
Research Articles