In vitro cytotoxic activity of Cymbopogon citratus L. and Cymbopogon nardus L. essential oils from Togo

Authors

  • Koffi Koba Unité de Recherche sur les Agroressources et la Santé Environnementale, Ecole Supérieure d?Agronomie, Université de Lomé, BP. 20131
  • Komla Sanda Unité de Recherche sur les Agroressources et la Santé Environnementale, Ecole Supérieure d?Agronomie, Université de Lomé, BP. 20131
  • Catherine Guyon Equipe des Sciences Séparatives, Biologiques et Pharmaceutiques, IFR 133, Faculté de Médecine et de Pharmacie, Université de Franche-Comté, Place Saint-Jacques, 25030, Besançon
  • Christine Raynaud Laboratoire de Chimie Agro-Industrielle, Arômes et Métrologie Sensorielle, UMR 1010, INP-ENSIACET, 118, route de Narbonne, 31077 Toulouse cedex
  • Jean-Pierre Chaumont Equipe des Sciences Séparatives, Biologiques et Pharmaceutiques, IFR 133, Faculté de Médecine et de Pharmacie, Université de Franche-Comté, Place Saint-Jacques, 25030, Besançon
  • Laurence Nicod Equipe des Sciences Séparatives, Biologiques et Pharmaceutiques, IFR 133, Faculté de Médecine et de Pharmacie, Université de Franche-Comté, Place Saint-Jacques, 25030, Besançon

DOI:

https://doi.org/10.3329/bjp.v4i1.1040

Keywords:

Cymbopogon citratus, Cymbopogon nardus, Cytotoxicity, Essential oil, HaCaT cell line

Abstract

The leaf essential oils of Cymbopogon citratus L. and Cymbopogon nardus L. (Poaceae) from Togo were steam-distilled, analyzed for percentage composition and investigated in vitro for their potential cytotoxic activity on human epidermic cell line HaCat. The percentage composition showed that the main constituents of essential oils samples were respectively geranial (45.2%), neral (32.4%) and myrc¨ne (10.2%) for C. citratus essential oil and citronellal (35.5%), geraniol (27.9%) and citronellol (10.7%) for that of C. nardus. The in vitro cytotoxicity bioassays on human epidermic cell line HaCaT revealed that the toxicity of the essential oil from C.citratus (IC50: 150 µL.mL-1) was higher than that of the essential oil from C.nardus (IC50: 450 µL.mL-1). Pure commercial neral, geranial, and citronellal standards showed respectively the following IC50 values: 100, 250 and 300 µL.mL-1). Conversely, pure citronellol standard appeared almost non-toxic (IC50>1000 µL.mL-1), proving the major role played in synergy by neral and geranial in the overall toxicity showed by the citratus oil sample tested in this work.

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Additional Files

Published

2008-08-16

How to Cite

Koba, K., K. Sanda, C. Guyon, C. Raynaud, J.-P. Chaumont, and L. Nicod. “In Vitro Cytotoxic Activity of Cymbopogon Citratus L. and Cymbopogon Nardus L. Essential Oils from Togo”. Bangladesh Journal of Pharmacology, vol. 4, no. 1, Aug. 2008, pp. 29-34, doi:10.3329/bjp.v4i1.1040.

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Research Articles