Induction of Cellulase Biosynthesis by Cellobiose Octaacetate in <i>Aspergillus humicola</i>

Authors

  • Meher Nigad Nipa Department of Microbiology, University of Chittagong, Chittagong 4331, Bangladesh
  • Sharmin Sultana Department of Microbiology, University of Chittagong, Chittagong 4331, Bangladesh
  • M Abdul Hakim Department of Microbiology, University of Chittagong, Chittagong 4331, Bangladesh

DOI:

https://doi.org/10.3329/bjm.v23i2.889

Keywords:

Aspergillus humicola, Carboxymethylcellulase (CMCase), Carboxymethylcellulose (CMC), Cellobiose octaacetate (COA)

Abstract

Aspergillus humicola, one of the major cellulase-producing fungi, was used in this study for carboxymethylcellulase (CMCase) production using Winstead's basal broth supplanted with cellobiose octaacetate (COA), a synthetic carbon source. Under all conditions, the enzyme biosynthesis was remarkably increased when the inducer COA was added to the production medium containing carboxymethylcellulose (CMC). Maximum enzyme production (1.62 U/ml) was achieved in COA-containing at 37°C. The enzyme production was highest at initial pH 5.5 and after 7 days incubation. The enzyme exhibited maximum activity at 40°C with a reaction pH 5.5. CMCase activity was inhibited by its own substrate CMC at concentration higher than 1.0%. The study clearly demonstrated that COA is a good inducer for extracellular CMCase production by the fungus.

Keywords: Aspergillus humicola, Carboxymethylcellulase (CMCase), Carboxymethylcellulose (CMC), Cellobiose octaacetate (COA)

DOI: http://dx.doi.org/10.3329/bjm.v23i2.889

Bangladesh J Microbiol, Volume 23, Number 2, December 2006, pp 174-176

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How to Cite

Nipa, M. N., Sultana, S., & Hakim, M. A. (2008). Induction of Cellulase Biosynthesis by Cellobiose Octaacetate in <i>Aspergillus humicola</i>. Bangladesh Journal of Microbiology, 23(2), 174–176. https://doi.org/10.3329/bjm.v23i2.889

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Short Communications