Effect of Culture Conditions on Production of Cytotoxic Enterotoxin of <i>Aeromonas sobria</i>
Keywords:Aeromonas sobria, cytotoxic enterotoxin (Act), Vero cell-assay
Cytotoxic enterotoxin (Act) production of a cytotoxic enterotoxin (act) gene-positive isolate of Aeromonas sobria was studied in different bacteriological culture media, such as brain heart infusion (BHIB), trypticase soy broth with yeast extract (TSB), casamino acid yeast extract and Richardson's medium. Cytotoxin production was evaluated in Vero cell-line. Of the various media tested, higher titre of Act was produced in BHIB. Production of Act was enhanced when calcium (1 mM), Mg (10 mM), iron (200 ìM) and sodium chloride (0.5%) was supplemented in BHIB.
Keywords: Aeromonas sobria, cytotoxic enterotoxin (Act), Vero cell-assay
Bangladesh J Microbiol, Volume 23, Number 2, December 2006, pp 171-173