Effect of Culture Conditions on Production of Cytotoxic Enterotoxin of <i>Aeromonas sobria</i>

Authors

  • Zeaur Rahim Centre for Health & Population Research, International Centre for Diarrhoeal Disease Research, Bangladesh (ICDDR,B), GPO Box 128, Mohakhali, Dhaka 1212, Bangladesh
  • Sirajul Islam Khan Department of Microbiology, University of Dhaka, Dhaka 1000, Bangladesh
  • Qazi Shafi Ahmed Centre for Health & Population Research, International Centre for Diarrhoeal Disease Research, Bangladesh (ICDDR,B), GPO Box 128, Mohakhali, Dhaka 1212, Bangladesh
  • Khan M Nasirul Islam Centre for Health & Population Research, International Centre for Diarrhoeal Disease Research, Bangladesh (ICDDR,B), GPO Box 128, Mohakhali, Dhaka 1212, Bangladesh

DOI:

https://doi.org/10.3329/bjm.v23i2.888

Keywords:

Aeromonas sobria, cytotoxic enterotoxin (Act), Vero cell-assay

Abstract

Cytotoxic enterotoxin (Act) production of a cytotoxic enterotoxin (act) gene-positive isolate of Aeromonas sobria was studied in different bacteriological culture media, such as brain heart infusion (BHIB), trypticase soy broth with yeast extract (TSB), casamino acid yeast extract and Richardson's medium. Cytotoxin production was evaluated in Vero cell-line. Of the various media tested, higher titre of Act was produced in BHIB. Production of Act was enhanced when calcium (1 mM), Mg (10 mM), iron (200 ìM) and sodium chloride (0.5%) was supplemented in BHIB.

Keywords: Aeromonas sobria, cytotoxic enterotoxin (Act), Vero cell-assay  

DOI: http://dx.doi.org/10.3329/bjm.v23i2.888

Bangladesh J Microbiol, Volume 23, Number 2, December 2006, pp 171-173

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How to Cite

Rahim, Z., Khan, S. I., Ahmed, Q. S., & Islam, K. M. N. (2008). Effect of Culture Conditions on Production of Cytotoxic Enterotoxin of <i>Aeromonas sobria</i>. Bangladesh Journal of Microbiology, 23(2), 171–173. https://doi.org/10.3329/bjm.v23i2.888

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Section

Short Communications